Mass cytometry
Human bone marrow assessment by single-cell RNA sequencing, mass cytometry, and flow cytometry
... Additional aliquots of bone marrow aspirate from this cohort together with paired blood samples, that were not yet analyzed, have been stored. Should transformative technologies emerge over the next few years, we would ...
10
Mass Cytometry of the Human Mucosal Immune System Identifies Tissue- and Disease-Associated Immune Subsets
... Procedures for mass cytometry antibody staining and data acquisition were carried out as previously described (Bendall et al., 2011). Briefly, directly after biopsy processing, we resuspended cells in cell ...
14
Mass cytometry as a platform for the discovery of cellular biomarkers to guide effective rheumatic disease therapy
... typical mass cytometry experiment contains up to 40 measured parameters per cell, yielding a high-dimensional and quantitative analysis of complex cellular networks, and may span multiple patient groups, ...
9
Mass cytometry reveals innate lymphoid cell differentiation pathways in the human fetal intestine
... High-dimensional mass cytometry provides an opportunity to analyze the heterogeneity and potential differentiation pathways of human ILCs in an unbiased and data-driven fashion based on the simultaneous ...
14
Multipanel mass cytometry reveals anti–PD-1 therapy–mediated B and T cell compartment remodeling in tumor-draining lymph nodes
... multipanel mass cytometry to obtain a high-parameter proteomic (39 total unique markers) immune profile of the TDLNs in a well-studied PD-1–responsive, immunocompetent mouse ...
16
Mass cytometry identifies distinct CD4+ T cell clusters distinguishing HIV-1–infected patients according to antiretroviral therapy initiation
... Recent guidelines recommend antiretroviral therapy (ART) to be administered as early as possible during HIV-1 infection. Few studies addressed the immunological benefit of commencing ART during the acute phase of ...
15
Lymphocyte mass cytometry identifies a CD3–CD4+ cell subset with a potential role in psoriasis
... Mass cytometry offers the opportunity to simultaneously analyze dozens of single-cell markers in complex cellular samples, resulting in highly complex data sets ...flow cytometry data analysis are ...
18
A comparison framework and guideline of clustering methods for mass cytometry data
... evaluation, “ coherence ” as internal evaluation, and stability, of nine methods based on six independent benchmark datasets. Seven unsupervised methods (Accense, Xshift, PhenoGraph, FlowSOM, flowMeans, DEPECHE, and ...
18
Development of a 2-dimensional atlas of the human kidney with imaging mass cytometry
... Imaging mass cytometry (IMC) is a technology that supports simultaneous detection of more than 40 protein markers on a single section of formalin-fixed, paraffin-embedded tissue, with the resultant ...
21
Identification of enhanced IFN-γ signaling in polyarticular juvenile idiopathic arthritis with mass cytometry
... percentages. Mass cytometry was used to assess the distribution of distinct groups of immune cells in the peripheral blood of treatment-naive polyarticular JIA patients and healthy controls as well as a ...
15
Signaling monitoring and flow cytometry
... Monitoring of cell signaling pathways by mass cytometry. …[r] ...
30
Strategies for improving the reporting of human immunophenotypes by flow cytometry
... Flow cytometry has become a foundational tool to analyze the immune system. The emergence of multiparametric analyses using novel fluorochromes has greatly expanded our ability to dynamically characterize a broad ...
5
Flow: Statistics, visualization and informatics for flow cytometry
... Flow cytometry is a high throughput method extensively used in both experimental and clinical settings for charac- terizing cell phenotypes, especially in the disciplines of hematology, immunology and infectious ...
12
EdU Flow Cytometry Kit. User Manual
... Samples of HeLa cells treated without (1A) or with EdU (1B) were incubated with 10 µM EdU for 2 hours. The click reaction using 6-FAM Azide was performed according to the recommended staining protocol. Fluorescence ...
12
A measure of endosomal pH by flow cytometry in Dictyostelium
... 0.1% sodium azide (NaN 3 ), as indicated. FITC fluorescence was then measured in a Fluorescence Activated Cell Sorter (FACS- Calibur). Only sodium azide and ammonium choride increased lysosomal pH, as revealed by an ...
8
Using Flow Cytometry to Analyze Cryptococcus Infection of Macrophages.
... Flow cytometry is a powerful analytical technique, which is increasingly being used to study the interaction between host cells and intracellular ...Flow cytometry is capable of measuring a greater number ...
13
Dual parameter flow cytometry studies in human lymphomas
... flow cytometry studies using Coulter volume and cell DNA content were carried out in monodisperse cell suspensions of 64 samples of human lymphoma, chronic lymphocytic leukemia, hairy cell leukemia, and benign ...
15
Rapid susceptibility testing of Candida albicans by flow cytometry
... Determination of the susceptibility of C. albicans to antifun- gal agents by flow cytometry. Figure 4 indicates the percentage of AO-labeled C. albicans organisms detected in regions R2 and R3 with or without ...
6
A novel statistical analysis and interpretation of flow cytometry data
... In this document, we have described and analyzed a recent class of mathematical models which combines the cyton model of population generation structure with a mass-conservation model of label dynamics. Unlike ...
39
Quantification of cellular viability by automated microscopy and flow cytometry
... flow cytometry- or epifluorescence microscopy- based assays, ...flow cytometry, culture supernatants were discarded and cells were detached with 30 μL TrypLE™ Express per well, then resuspended in 30 μL of ...
9