MHC class I protein
The Major Histocompatibility Complex Class I Genes of Zebrafish.
... the MHC class I protein from the cell membrane but weak enough as to maintain any interactions with ...β2M. I have employed NP-40 and Triton-X based lysis buffers and different ...
156
Mouse Norovirus Infection Reduces the Surface Expression of Major Histocompatibility Complex Class I Proteins and Inhibits CD8+ T Cell Recognition and Activation
... in MHC class I surface expression in cells that were infected with MNV (NS5 positive) from that in mock- infected cells ...of MHC class I expression was evaluated by measuring ...
18
Altered Expression of TAP-1 and Major Histocompatibility Complex Class I in Laryngeal Papillomatosis: Correlation of TAP-1 with Disease
... (MHC) class I protein expression in laryngeal papilloma tissue biopsies and cell culture of primary ...lating class I MHC cell surface expression via decreased TAP-1 ...
7
A Novel Bat Herpesvirus Encodes Homologues of Major Histocompatibility Complex Classes I and II, C-Type Lectin, and a Unique Family of Immune-Related Genes
... (iv) MHC-related proteins. Some betaherpesviruses encode multiple MHC class I homologues, such as UL18 and UL142 of HCMV (24) and CCMV (21), m144 of MCMV (64), R144 of RCMV (89), and gp147, ...
17
Revised 43_Lecture_Presentation_PC.ppt
... • Cytotoxic T cells recognize fragments of foreign proteins produced by infected cells and possess an accessory protein that binds to class I MHC molecules. • The activated cytotoxic [r] ...
62
Modulation of cellular macromolecular synthesis by coronavirus: implication for pathogenesis.
... In contrast to decreased MHC class I expression, the expression of the JHMV S protein on the cell surface of infected J774.1 cells was clearly detectable at 8 h p.i.. and increased with [r] ...
5
Effect of the Attenuating Deletion and of Sequence Alterations Evolving In Vivo on Simian Immunodeficiency Virus C8-Nef Function
... Nef protein could be ...function. Protein stability and the ability of Nef to downregulate CD4 and MHC class I surface expres- sion and to enhance virion infectivity and viral ...
8
Posttranscriptional Inhibition of Class I Major Histocompatibility Complex Presentation on Hepatocytes and Lymphoid Cells in Chronic Woodchuck Hepatitis Virus Infection
... the class I MHC gene transcription in the liver and the spleen could be due to an innate difference in the levels of the gene expression between these two ...that class I heavy-chain ...
12
Transcriptional Changes Induced by Bovine Papillomavirus Type 1 in Equine Fibroblasts
... complex class I (MHC-I) protein, was confirmed using other BPV-1-transformed cell ...and MHC I showed robust down-regulation in all ...the protein level for MMP-1 ...
11
Association of CD99 short and long forms with MHC class I, MHC class II and tetraspanin CD81 and recruitment into immunological synapses
... anti-MHC class I mAb HC10 (IgG1), anti-MHC class II mAb MEM-136 (IgG1), anti-CD81 mAb M38 (IgG1), anti-NTAL mAb NAP-07 (IgG1), anti-Lck mAb Lck-01 (IgG1) and anti-CD45 mAb MEM-55 (IgG1) ...
13
MHC class I multimers
... fusion protein containing the extracellular domains of a MHC class I heavy chain together with a 13-amino acid C- terminal tag (that allows site-specific biotinylation), is expressed in ...
5
Approaches to Improve Chemically Defined Synthetic Peptide Vaccines
... However, these approaches also have their constraints. For reporter proteins, the main constraint is that degradation is the hallmark of antigen (cross-)presentation: during antigen presentation any protein must ...
8
DNMT1 regulates expression of MHC class I in post-mitotic neurons
... B27, Glutamax and HEPES containing 10 μM Ara-C and 1 μM siRNA, but excluding BSA. Transfection efficiency was assessed using a Cy3-labelled non-targeting siRNA (Additional file 1: Figure S1). For studies of the effect of ...
16
Characterization of a Murine Cytomegalovirus Class I Major Histocompatibility Complex (MHC) Homolog: Comparison to MHC Molecules and to the Human Cytomegalovirus MHC Homolog
... the protein product with the antibody ...of protein from positive clones revealed two bands, one having an apparent molecular mass of 45 kDa (consistent with its identity as truncated m144) and the other ...
7
Gag-specific cytotoxic T lymphocytes from human immunodeficiency virus type 1-infected individuals: Gag epitopes are clustered in three regions of the p24gag protein.
... In this report we investigate the number of T-cell epitopes in the Gag protein that are recognized by CTL from a sample of HIV-1-infected individuals with MHC class I polymorphisms that [r] ...
9
Viral gene inhibition of class I major histocompatibility antigen expression: not a general mechanism governing the tumorigenicity of adenovirus type 2-, adenovirus type 12-, and simian virus 40-transformed Syrian hamster cells.
... In these studies, there was no association between the low levels of class I MHC protein expressed by three of these Adl2transformed cell lines and their tumorigenicity in newborn The El[r] ...
7
The newly arisen Devil facial tumour disease 2 (DFT2) reveals a mechanism for the emergence of a contagious cancer
... MHC class I transcripts were aligned and translated into protein sequences using CLC ...expressed MHC class I by western blot as described ...
18
Structural and Functional Dissection of Human Cytomegalovirus US3 in Binding Major Histocompatibility Complex Class I Molecules
... type I transmembrane glycoproteins featuring a short cytoplasmic tail and a bulky luminal ...the MHC class I binding function and the ER retention function of US3 are assigned to different ...
8
Herpes B Virus, Macacine Herpesvirus 1, Breaks Simplex Virus Tradition via Major Histocompatibility Complex Class I Expression in Cells from Human and Macaque Hosts
... (MHC) class I in order to evade CD8 ⴙ T-cell activation is characteristic of most ...total protein expression of MHC class I molecules in B virus-infected human foreskin ...
9
Expression of m157, a Murine Cytomegalovirus-Encoded Putative Major Histocompatibility Class I (MHC-I)-Like Protein, Is Independent of Viral Regulation of Host MHC-I
... cells were infected as described for Fig. 1, with MCMV at MOI of 5. Total RNA was isolated at the times indicated after infection, using TRIZOL reagent (Life Technologies) according to the manufacturer’s protocol. The ...
6