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Microscopy, Fluorescence

Superresolution fluorescence microscopy for 3D reconstruction of thick samples

Superresolution fluorescence microscopy for 3D reconstruction of thick samples

... superresolution fluorescence microscopy based on single-molecule localization has been just a few micrometers, this is a significant improvement which is expected to open a way to wide application of ...

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SPOT DETECTION METHODS IN FLUORESCENCE MICROSCOPY IMAGING: A REVIEW

SPOT DETECTION METHODS IN FLUORESCENCE MICROSCOPY IMAGING: A REVIEW

... Fluorescence microscopy imaging has become one of the essential tools used by biologists to visualize and study intracellular particles within a ...of microscopy image analysis, consisting of ...

18

Fluorescence Microscopy of Nicotinic Acetylcholine Receptors

Fluorescence Microscopy of Nicotinic Acetylcholine Receptors

... confocal microscopy led to challenges to data interpretation that were similar to those seen with other methods using confocal microscopy: FRET signals were homogenous for all experimental conditions and it ...

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Fluorescence Optofluidic Microscopy and Fluorescence Microscopy Based on the Talbot Effect

Fluorescence Optofluidic Microscopy and Fluorescence Microscopy Based on the Talbot Effect

... chip fluorescence microscope system, termed fluorescence optofluidic microscope ...of fluorescence microscopy imaging of samples in fluid ...the fluorescence emissions are collected by ...

122

Three photon light sheet fluorescence microscopy

Three photon light sheet fluorescence microscopy

... transform along the axial direction (Fig. 3(b)). The MTF con- cisely represents information of both resolution and contrast. We set a practical noise-floor at 5% contrast to determine the maximum axial resolution, which ...

6

Measuring DNA content in live cells by fluorescence microscopy

Measuring DNA content in live cells by fluorescence microscopy

... data collected from flow cytometry. Therefore, we per- formed a side-by-side experiment comparing asynchro- nous cell cycle distributions acquired in live cells by fluorescence microscopy to that of fixed ...

10

Mobile Digital Fluorescence Microscopy for Diagnosis of Tuberculosis

Mobile Digital Fluorescence Microscopy for Diagnosis of Tuberculosis

... smear microscopy in high-tuberculosis (TB)-burden regions is limited by a scarcity of microscopes and experi- enced ...digital fluorescence microscope that may expand access to ...smear microscopy ...

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Direct Interaction of Baculovirus Capsid Proteins VP39 and EXON0 with Kinesin-1 in Insect Cells Determined by Fluorescence Resonance Energy Transfer-Fluorescence Lifetime Imaging Microscopy

Direct Interaction of Baculovirus Capsid Proteins VP39 and EXON0 with Kinesin-1 in Insect Cells Determined by Fluorescence Resonance Energy Transfer-Fluorescence Lifetime Imaging Microscopy

... scanning microscopy (CLSM) as previously ...Two-photon fluorescence lifetime imaging analysis of transfected insect ...Two-photon-induced fluorescence lifetime images were ob- tained with multiphoton ...

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Frontiers in fluorescence microscopy

Frontiers in fluorescence microscopy

... a fluorescence microscope is no longer limited by the wavelength of the light, as postulated by ...(STED) microscopy is to make the fluorescent spot smaller by quenching the excited molecules at the ...

12

Imaging Flies by Fluorescence Microscopy: Principles, Technologies, and Applications

Imaging Flies by Fluorescence Microscopy: Principles, Technologies, and Applications

... fluorescence microscopy-based approaches on cell membranes, organelles, and other subcellular struc- tures serves as important landmarks to determine the intra- cellular localization of proteins, monitor the ...

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Multidimensional fluorescence microscopy of multiple organelles in Arabidopsis seedlings

Multidimensional fluorescence microscopy of multiple organelles in Arabidopsis seedlings

... confocal microscopy, physical interac- tions between the nucleus and plastids were recently described in tobacco hypocotyl epidermal cells [11], and signal transduction between the nucleus and plastids has also ...

14

Smear microscopy and culture conversion rates among smear positive pulmonary tuberculosis patients by HIV status in Dar es Salaam, Tanzania

Smear microscopy and culture conversion rates among smear positive pulmonary tuberculosis patients by HIV status in Dar es Salaam, Tanzania

... Patient recruitment was done by the District Tuberculo- sis and Leprosy Coordinators (DTLCs) of the respective health facilities. Recruitment was continuous throughout the study period. All new smear positive pulmonary ...

6

Light sheet fluorescence microscopy for neuroscience

Light sheet fluorescence microscopy for neuroscience

... acquisition of volumetric images very long. The wide light exposure combined with the limited acquisition speed results in a much higher exposure of the sam- ple causing potential photobleaching. Moreover, high ...

46

Apertureless near field optical microscopy for fluorescence imaging

Apertureless near field optical microscopy for fluorescence imaging

... effects. Fluorescence quen- ching could be identified by taking fluorescence lifetime measurements using a pulsed laser and measuring the delay between a laser pulse and the fluorescent photons emitted by ...

142

Anisotropic Diffusion in Fluorescence Microscopy

Anisotropic Diffusion in Fluorescence Microscopy

... FRAP (Fluorescence Recovery After Photobleaching) techniques can be used to measure the effective diffusion coefficients of tracer molecules within biological mate- rials like articular cartilage [1]. Because of ...

35

Bioprobes Based on Aptamer and Silica Fluorescent Nanoparticles for Bacteria Salmonella typhimurium Detection

Bioprobes Based on Aptamer and Silica Fluorescent Nanoparticles for Bacteria Salmonella typhimurium Detection

... and fluorescence microscopy pictures of group A bacteria, ...and fluorescence microscopy pictures of group B bacteria, ...and fluorescence microscopy pictures of group C ...

9

Multimodal and multiscale optical imaging of nanomedicine delivery across the blood-brain barrier upon sonopermeation

Multimodal and multiscale optical imaging of nanomedicine delivery across the blood-brain barrier upon sonopermeation

... 4: Fluorescence microscopy analysis of nanocarrier accumulation, extravasation and penetration in the brain upon ...Fluorescent microscopy images of brain sections from control and treated animals ...

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Madhira, Sushi
  

(2017):


	Single-molecule multi-color FRET: from DNA to protein.


Dissertation, LMU München: Fakultät für Chemie und Pharmazie

Madhira, Sushi (2017): Single-molecule multi-color FRET: from DNA to protein. Dissertation, LMU München: Fakultät für Chemie und Pharmazie

... Single-molecule fluorescence techniques are a powerful tool that enable monitoring of the conformational dynamics of biomolecules in real time, whether in solution or tethered to the ...reflection ...

168

A fluorescence lifetime-based fibre-optic glucose sensor using glucose/galactose-binding protein

A fluorescence lifetime-based fibre-optic glucose sensor using glucose/galactose-binding protein

... beads. Fluorescence lifetime increased in response to glucose, observed by fluorescence lifetime imaging microscopy of the ...fibre. Fluorescence lifetime responses were recorded using pulsed ...

5

Comparison of Single Sputum Smear (Home Collection) by Fluorescent Microscopy Vs Two Smears by Ziehl Neelsen Method (Routine RNTCP)

Comparison of Single Sputum Smear (Home Collection) by Fluorescent Microscopy Vs Two Smears by Ziehl Neelsen Method (Routine RNTCP)

... of fluorescence microscopy using Fluoreslen S by a blinded rechecking trial in Nairobi, Kenya ...fluorescent microscopy equipment are the major reasons why they are rarely used for sputum smear ...

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