Molecular typing
External Quality Assessment of Molecular Typing of Staphylococcus aureus Isolates by a Network of Laboratories
... of molecular methods for epidemiological typing of nosocomial bacterial pathogens has become a standard service for hospital infection control programs ...in-house molecular typing methods and ...
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Molecular Typing and Distribution of Staphylococcus aureus Isolates in Eastern Canadian Dairy Herds
... Preparation of genomic DNA. Genomic DNA was prepared as described by Bannerman et al. (7), with modifications as suggested by the Centers for Disease Control pulse net laboratory protocol for molecular ...
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Use of the Agilent 2100 Bioanalyzer for Rapid and Reproducible Molecular Typing of Streptococcus pneumoniae
... for molecular typing but has the drawback of difficulties in accurately sizing DNA fragments and comparing banding patterns on agarose ...for typing of the important human pathogen Streptococcus ...
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Multilocus Sequence Typing Compared to Pulsed Field Gel Electrophoresis for Molecular Typing of Pseudomonas aeruginosa
... For hospital epidemiologists, determining a system of typing that is discriminatory is essential for measuring the effectiveness of infection control measures. In situations in which the incidence of resistant ...
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Molecular typing of Yersinia pseudotuberculosis by using an IS200 like element
... IS typing with two other molecular typing methods, pulsotyping and ...IS typing was similar to that of pulsotyping, which is a time- consuming method; however, the two methods were comple- ...
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Improved Molecular Typing Assay for Rhinovirus Species A, B, and C
... Phylogenetic analysis and type assignments based on 5=- UTR sequences. Our RV molecular typing assay is based on phy- logenetic analysis of an ⬃ 270-bp-long fragment internal to the P1-P2 PCR amplicon (Fig. ...
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Molecular Typing of Clinical and Environmental Cryptococcus neoformans Strains Isolated in Italy
... Several molecular typing methods have been used for studying the molecular epidemiology of ...sequence typing (MLST) [8]. DNA mo- lecular typing techniques divide ...major ...
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Diversity of Potential Short Tandem Repeats in Mycobacterium leprae and Application for Molecular Typing
... Poor understanding of leprosy transmission has hindered us from eradicating the disease even though M. leprae was iden- tified as the pathogen as early as 1873 by Hansen (19). The unusual biological traits of being ...
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Molecular Typing of Mycobacterium abscessus Based on Tandem Repeat Polymorphism
... Choi et al. used two tandem-repeat loci to distinguish Mycobacte- rium abscessus from other mycobacterial species and discrimi- nated Mycobacterium abscessus, Mycobacterium massiliense, and Mycobacterium bolletii by ...
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Evaluation of Ribotyping as a Tool for Molecular Typing of Yersinia pseudotuberculosis Strains of Worldwide Origin
... pseudotuberculosis patterns. Overall, the Y. pseudotuberculosis patterns resembled those of Y. pestis. In particular, the con- served and highly conserved hybridizing bands in Y. pseudotuber- culosis were present and ...
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Multiple Polymorphic Loci for Molecular Typing of Strains of Mycobacterium leprae
... required. Molecular typing will make it feasible to study the global and geographical distribu- tions of distinct clones of ...develop molecular typing meth- ods to complement the efforts of ...
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Molecular Typing of Mycobacterium bovisIsolates from Cameroon
... At the level of this study, spoligotyping seems promising for high-throughput molecular typing of M. bovis isolates in Cam- eroon. By using PFGE and RFLP with IS6110-RHS in combi- nation with spoligotyping, ...
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Review on Molecular Typing Methods of Pathogens
... common molecular typing methods, Pulsed-Field Gel Electrophoresis (PFGE), Restriction Fragment Length Polymorphisma (RFLP), Random Amplified Polymorphic DNA (RAPD), Automatic Ribotyping, rep-PCR, ...
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Molecular Typing of Salmonella Isolates in Poultry by Pulsed-Field Gel Electrophoresis in Iran
... lecular typing of ...phenotypic typing methods have been found to lack discriminatory power due to the expanded diversity of ...reason, molecular typing methods have been developed for the ...
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Molecular typing methods used in studies of Mycobacterium tuberculosis in Iran: a systematic review
... each typing system has its own advan- tages and disadvantages, and it is difficult to decide clearly which of them is superior to ...ideal molecular typing method must comply with the needs of ...
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Molecular Typing and Resistance Analysis of Travel Associated Salmonella enterica Serotype Typhi
... Our molecular typing data shed light on the distribution pat- terns and the dominant S. Typhi types circulating throughout the globe. Twenty strains had the ribotype R6, making it the predom- inant type in ...
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Development of a Multilocus Sequence Typing Scheme for Molecular Typing of Mycoplasma pneumoniae
... by molecular methods is of impor- tance for the understanding of the epidemiology of ...that molecular typing of ...Initial molecular typing targeted the gene en- coding the major ...
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Francisella tularensis Molecular Typing Using Differential Insertion Sequence Amplification
... Tularemia is a potentially fatal disease that is caused by the highly infectious and zoonotic pathogen Francisella tularensis. Despite the monomorphic nature of sequenced F. tularensis genomes, there is a significant ...
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Nosocomial Plasmodium falciparum infections confirmed by molecular typing in Medellín, Colombia
... of molecular typing of ...the molecular findings together with the epidemiological characteristics contrib- ute to confirm nosocomial transmission of the ...
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Molecular Typing of Selected Enterococcus faecalis Isolates: Pilot Study Using Multilocus Sequence Typing and Pulsed Field Gel Electrophoresis
... sequence typing (MLST) approach with a well-established molecular typing technique, pulsed-field gel electrophoresis (PFGE), for subspecies differen- tiation of Enterococcus faecalis ...
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