mouse preimplantation
Requirement of the MAP kinase signaling pathways for mouse preimplantation development
... We then examined whether p38 and JNK are activated during mouse preimplantation development. Activation of p38 α can be assessed by using anti-phospho-specific p38 α antibody. Indirect immunofluorescence ...
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Requirement for ERK MAP kinase in mouse preimplantation development
... during mouse preimplantation development, embryos were stained with anti-phospho-ERK antibody and anti-phospho-ELK1 ...in mouse preimplantation development, and that U0126 is able to inhibit ...
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The cytotoxic effects of dimethyl sulfoxide in mouse preimplantation embryos: a mechanistic study
... on mouse preimplantation embryos. During in vitro growth of mouse zygotes to blastocyst stage embryos, exposure to different concentrations of DMSO greatly increased the osmolarity of KSOM, ...
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Survival Assessment of Mouse Preimplantation Embryos After Exposure to Cell Phone Radiation
... Luo et al. (2006) evaluated the effects of extreme- ly low frequency electromagnetic fields (ELF- EMFs) exposure on mouse preimplantation em- bryos. Their results showed a significant delay in the cleavage ...
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Histone variant H3 3 maintains a decondensed chromatin state essential for mouse preimplantation development
... study mouse preimplantation development (Chen et ...in mouse allows us to design MOs expected to target ...early mouse embryos, similar to observations in Drosophila Kc cells (McKittrick et ...
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Characterization of a diverse secretome generated by the mouse preimplantation embryo in vitro
... the mouse preimplantation embryo in ...for preimplantation embryo development (NLR family, pyrin domain containing 5 and peptidyl arginine deiminase, type ...the preimplantation embryo in ...
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Single cell RNA seq transcriptome analysis of linear and circular RNAs in mouse preimplantation embryos
... our mouse preimplantation embryo data ...the mouse preimplantation ...in mouse pre- implantation embryos in vivo, similar to that in cultured cells in ...
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Lineage specification in the mouse preimplantation embryo
... It is generally considered that until the 8-cell stage each blastomere retains totipotency – the ability to generate all three lineages (TE, EPI and PE). By aggregating one or two donor blastomeres with a genetically ...
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Role Of Maternal Sin3a In Reprogramming Gene Expression During Mouse Preimplantation Development
... maturing mouse oocytes, macroH2A is associated with chromosomes and is lost from the embryo following fertilization, specifically by the late 2 pronuclei stage (Chang et ...
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Distribution of p53 binding protein 1 (53BP1) and phosphorylated H2A X during mouse preimplantation development in the absence of DNA damage
... most abundant H2A variant, and the ability to remodel the sperm nucleus to form a paternal pronucleus after fertilisation is directly associated with phosphorylation of H2A.X (Dimitrov et al., 1994). This suggests that ...
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Perturbations in imprinted methylation from assisted reproductive technologies but not advanced maternal age in mouse preimplantation embryos
... While the focus of this study was centered on imprinted methylation, the possibility exists that advanced maternal age may impair DNA methylation or histone modifications at other regions of the genome. In one study, ...
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Contribution of JAM 1 to epithelial differentiation and tight junction biogenesis in the mouse preimplantation embryo
... these models, cell fate diversification can occur by atypical PKC phosphorylation of cytoskeletal components, resulting in different inheritance of determinant proteins (Betschinger et al., 2003). Similar to the current ...
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Rabbit antiserum to mouse embryonic stem cells delays compaction of mouse preimplantation embryos
... medium with or without antiserum for 12-36 h. The ratios of in vitro embryo development of the blastocysts, cell division, attachment potential, alkaline phosphatase activity, post-implantation development, and chimera ...
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The Role of p66Shc in Mouse Blastocyst Development
... in mouse preimplantation embryos by siRNA-mediated knockdown affected blastocyst development by altering cell fate ...mammalian preimplantation development and reveal a novel role of p66Shc in cell ...
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Transcription factor AP 2γ is a core regulator of tight junction biogenesis and cavity formation during mouse early embryogenesis
... To rule out any potential siRNA off-targeting effects, several experiments were carried out. First, we showed by qRT-PCR that the control siRNA did not target Tcfap2c transcripts in injected embryos (supplementary ...
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Life giving caspases: revealing new roles during mouse embryo preimplantation development
... in mouse embryos before the blastocyst stage, their role is unclear, since apoptosis does not occur significantly before ...used mouse preimplantation development as a model to evaluate the existence ...
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Mouse Obox and Crxos modulate preimplantation transcriptional profiles revealing similarity between paralogous mouse and human homeobox genes
... ulated, 125 commonly down-regulated). Much smaller overlap was detected for other pairs of mouse and human ETCHbox genes. There are differences between the bio- logical activities of the two genes, however. For ...
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Ginseng Extract Treatment Influences on Preimplantation Development in Vitro, and Pregnancy and Post-Partum Development in the Mouse
... the preimplantation embryos were directly exposed to the ginseng extract in culture, whereas concentrations of ginseng directly affecting embryos in vivo are unknown due to uncertainties regarding ginseng compound ...
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Molecular cytogenetic investigations into infertility, recurrent in vitro fertilisation failure and multiple miscarriage
... Preimplantation diagnosis offers couples with a risk of genetic disease an alternative to pre-natal diagnosis followed by a possible termination of pregnancy. This is particularly attractive to couples with ...
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Preimplantation genetic diagnosis and embryo research human developmental biology in clinical practice
... human preimplantation embryo. The advent of preimplantation genetic diagnosis and the frequent examination of embryos by molecular and cytogenetic techniques are providing more information daily (Munne et ...
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