Multiplex PCR
QuantiFast Multiplex PCR Handbook
... (QuantiFast Multiplex PCR Kit and QuantiFast Multiplex PCR +R Kit) is covered by one or more of the following US patents and corresponding patent claims outside the US: 6,127,155, 5,677,152 ...
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Detection and Identification ofActinobacillus pleuropneumoniae Serotype 5 by Multiplex PCR
... Serotyping of Actinobacillus pleuropneumoniae is based on detection of the serotype-specific capsular antigen. However, not all isolates can be serotyped, and some may cross-react with multiple serotyping reagents. To ...
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Use of a Genus and Species Specific Multiplex PCR for Identification of Enterococci
... multiplexing PCR was achieved by first circumventing the need for purifying template ...the PCR performed well with pure DNA, isolated colonies suspended in sterile water worked equally as well and required ...
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Identification of Bacillus anthracis by rpoB Sequence Analysis and Multiplex PCR
... Comparative sequence analysis was performed upon Bacillus anthracis and its closest relatives, B. cereus and B. thuringiensis. Portions of rpoB DNA from 10 strains of B. anthracis, 16 of B. cereus, 10 of B. ...
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Clinically Applicable Multiplex PCR for Four Middle Ear Pathogens
... reactions. Multiplex PCR product detection on ALFexpress sequenc- ing ...The PCR product detection on the sequencer was established by varying the proportion of labeled to unlabeled universal reverse ...
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Species Specific Identification of Human Adenoviruses by a Multiplex PCR Assay
... of PCR products (31, 50) is potentially more informative, this approach is beyond the scope of most diagnostic ...on multiplex PCR amplification of Ad species with species-specific primers to the Ad ...
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Usefulness of Seminested Multiplex PCR in Surveillance of Imported Malaria in Spain
... new PCR-based method for the diagnosis of malaria in the Spanish Malaria Reference Laboratory has promoted an increase in confirmed cases of ...seminested multiplex PCR permitted confirmation of the ...
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Multiplex PCR for Typing and Subtyping Influenza and Respiratory Syncytial Viruses
... enhanced sensitivity combined with rapid detection and sub- typing ability. RSV is more labile than influenza virus, which has made conventional virus culture more difficult. As a con- sequence, rapid diagnostic methods ...
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Multiplex PCR for identification of methicillin resistant staphylococci in the clinical laboratory
... A multiplex PCR assay for detection of the staphylococcal mecA gene the structural gene for penicillinbinding protein 2a was compared with agar dilution and disk diffusion susceptibility[r] ...
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Comparison of Two Multiplex PCR Systems for Meat Species Authentication
... simplex PCR resulting in specific amplicons for species of cattle, horse, pig, ostrich, and sheep with the specific PCR products of 311, 253, 100, 155, and 119 bp, respectively (Figure ...
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Robust SNP genotyping by multiplex PCR and arrayed primer extension
... of PCR, we designed 50× 5' linker PCR primer pairs [Additional file 6] based on a T m of 65°C ± 7°C and per- formed 50-plex PCR in one single reaction per ...new PCR primer had a common linker ...
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Detection of Virulence Genes of Clostridium difficile in Children with Cancer by Multiplex PCR
... Sample handling and DNA extraction. Fecal swabs were placed in 1.0 ml of TE buffer or DDW and vortexed several times. Then, the tube content was transferred to Eppendorf tubes and centrifuged at 1000-1500 rpm for 1-2 ...
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Practical Implementation of a Multiplex PCR for Acute Respiratory Tract Infections in Children
... Molecular testing for acute respiratory infections (ARIs) has documented value but limited implementation due to questions that typically slow the acceptance of new tests. This study sought to address these questions and ...
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Utility of a Multiplex PCR Assay for Detecting Herpesvirus DNA in Clinical Samples
... the multiplex PCR has enabled the testing of a wide range of specimen types, including blood and tissues for the detection of ...the PCR, where previously isolation of HSV from this site was not ...
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Rapid Identification of Yeasts in Positive Blood Cultures by a Multiplex PCR Method
... Finally, Turenne et al. determined that universal fungal primers ITS3 and ITS4 and an automated system of fluores- cent capillary electrophoresis could be used to determine the sizes of amplicons of the ITS2 genetic ...
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Multiplex PCR for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in Genitourinary specimens
... a multiplex PCR (M-PCR) assay for the simultaneous detection of Chlamydia trachomatis and Neisseria ...respectively. PCR products were easily detected by agarose gel electrophoresis and ...
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Detection and Typing of Human Papillomavirus by E6 Nested Multiplex PCR
... nested multiplex PCR (NMPCR) assay that combines degenerate E6/E7 consensus primers and type- specific primers was evaluated for the detection and typing of human papillomavirus (HPV) genotypes 6/11, 16, ...
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Development of a Multiplex PCR Assay for Detection and Genogrouping of Neisseria meningitidis
... a PCR block is the only major equipment ...this multiplex PCR-based assay has the advantage for applications in clinical diagnosis and epide- miological ...
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Discrimination of Major Capsular Types of Campylobacter jejuni by Multiplex PCR
... a multiplex PCR method to determine CPS type overcomes many of the complexities of the Penner scheme, and the method also does not require that the phase-variable CPS be expressed at the time of ...3. ...
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Multiplex PCR Method for Species Identification of Coagulase Positive Staphylococci
... In veterinary medicine, coagulase-positive staphylococci (CoPS) other than Staphylococcus aureus have frequently been misidentified as being S. aureus strains, as they have several phenotypic traits in common. There has ...
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