mutation detection
PIK3CA mutation detection in metastatic biliary cancer using cell-free DNA
... PIK3CA mutation is considered a good candidate for targeted therapies in cancers, especially biliary tract cancer ...patients, mutation detection using ddPCR with Bio-Rad’s PrimePCR mutation ...
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Genotypic Determination of Mycobacterium tuberculosisAntibiotic Resistance Using a Novel Mutation Detection Method, the Branch Migration Inhibition M tuberculosis Antibiotic Resistance Test
... of mutation would be very useful in antimicrobial susceptibility testing, when any of the multiple mutations within a given sequence can often give rise to drug ...new mutation detection method is ...
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Enzymatic assays and pcr-based dna fingerprinting in mutation detection
... RAPD fingerprinting has found wide-spread use in the fields of genetic mapping and genomic fingerprinting of a wide range of species. It has been used in epidemiology and infection control, microbial population genetics ...
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Targeted mutation detection in breast cancer using MammaSeq™
... in mutation detection by the MammaSeq™ panel, sequencing was carried out on a co- hort of 46 solid tumor samples, with a mean read depth of 2311× (Additional file 6: Figure ...
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Cancer gene mutation detection in circulating cell free DNA in blood
... EGFR mutation analysis on clinical ...existing mutation detection methods, to developing novel methods for EGFR mutant detection to address the short comings of existing ...for mutation ...
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IDH1 mutation detection by droplet digital PCR in glioma
... the detection of a mutant allele fraction as low as ...“ultra-sensitive detection method”, ddPCR was applied in the pretreatment of EGFR T790M mutations in non-small cell lung cancer patients ...[13]. ...
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Development of a Multiplex PCR and SHV Melting Curve Mutation Detection System for Detection of Some SHV and CTX M β Lactamases of Escherichia coli, Klebsiella pneumoniae, and Enterobacter cloacae in Taiwan
... Infection by extended-spectrum  -lactamase (ESBL)-pro- ducing Enterobacteriaceae has become a serious problem in Taiwan. According to previous reports from various local hos- pitals (7, 10, 15, 25, 27, 28, 30, 32), ...
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Mutation Detection in Machado-Joseph Disease Using Repeat Expansion Detection
... PCR are indicated below in italics. Two PCR alleles in the normal range are designated N. Individual A is clinically unaffected but show both RED and PCR expansions. Individuals marked B[r] ...
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Evaluation of denaturing gradient gel electrophoresis (DGGE) as a method of mutation detection
... The DGGE technique which gives good reproducibility and allows clear resolution of different mutations is applicable to such analyses. A distinctive banding pattern is observed for each mutation described in the ...
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Molecular genetics of hunter disease and the psuedodeficiency of arylsulphatase a
... (mutation detection enhancer) gel with or without glycerol in the cold room followed by detection of DNA bands by a silver staining protocol (see material and methods for detailed description of the ...
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Low BRAF V600 mutation prevalence in primary skin nodular melanoma in Indonesia: a real time PCR detection among Javanese patients
... BRAF mutation testing nor its inhibitor has been widely used in clinical management of melanoma cases in ...BRAF mutation detection for prognosis and therapy purposes in ...patients’ mutation ...
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Ciprofloxacin Resistance in Campylobacter jejuniIsolates: Detection of gyrA Resistance Mutations by Mismatch Amplification Mutation Assay PCR and DNA Sequence Analysis
... the mutation primer in this assay was developed to detect, we have not yet developed additional primers to detect other reported mutations in the ...PCR mutation detection primers as isolates with ...
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An investigation of genetic alterations in gastric and colorectal cancer
... making mutation detection a labour-intensive process (Groden et al, 1993; Miyoshi et a l, 1992a; Fodde et a l, ...a mutation dense region where somatic mutations in particular are found to cluster ...
312
MS analysis of single nucleotide differences in circulating nucleic acids: application to noninvasive prenatal diagnosis
... the detection or exclusion of the paternally inherited mutant allele in maternal ...fetal mutation detection in maternal plasma, such as achondroplasia, Huntington’s disease, cystic fibrosis, and ...
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Statistical tools for assessment of spatial properties of mutations observed under the microarray platform
... cluster detection under a genotyp- ing array probe ...for mutation detection compared to sequencing every base pair of the entire ...any mutation clusters occurring in such regions are ...
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Diagnostic utility of BRAFV600E mutation testing in thyroid nodules in elderly patients
... of detection methods and geographical ...BRAF mutation inducing factors have been identified so ...V600E mutation detection as a diagnostic tool to refine inconclusive FNC results in elderly ...
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Implementation of two high through-put techniques in a novel application: detecting point mutations in large EMS mutated plant populations
... the mutation detection rate is the most relevant information as it determines how many families/genotypes have to be screened before a mutant will be ...designing mutation breed- ing also the real ...
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Sensitive detection of BRAF V600E mutation by Amplification Refractory Mutation System (ARMS)-PCR
... V600E mutation testing, including dideoxy sequencing, colorimetric Mutector assay, allele-specific real-time PCR, pyrose- quencing, high resolution melting (HRM) analysis and COLD-PCR ...low-cost mutation ...
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Formulation and Evaluation of Polymeric Nanoparticles as Carriers of Rosuvastatin Calcium for Oral Administration
... Table IV Applications of various nanosystems in cancer therapy Nanosystem Applications in cancer therapeutics Carbon nanotubes DNA mutation detection, disease protein biomarker detection[r] ...
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Hemophilia B: Molecular pathogenesis and mutation analysis
... Somatic and gonadal mosaicism for hemophilia B (the existence of two or more cell lines in an individual with different genotypes) has been reported occasionally. Sommer et al. reported on 414 hemophilia B families among ...
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