Nonpermissive temperature
Replication of herpesvirus DNA. V. Maturation of concatemeric DNA of pseudorabies virus to genome length is related to capsid formation.
... The number of empty and full capsids containing electron-dense cores present in tsN-infected cells incubated at the nonpermissive temperature, as well as after shiftdown to the permissiv[r] ...
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Analysis of HCF, the Cellular Cofactor of VP16, in Herpes Simplex Virus-Infected Cells
... Intracellular localization of HCF and VP16 in tsBN67-in- fected cells. The biochemical analysis by coprecipitation indi- cated that at both MOIs, VP16 binding to HCF was defective in tsBN67 cells at the ...
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Herpes Simplex Virus Glycoproteins: Isolation of Mutants Resistant to Immune Cytolysis
... Tests with a temperature-sensitive ts mutant of HSV-1 tsAl defective in glycoprotein synthesis at the nonpermissive temperature 39°C demonstrated that over 90% of mutant-infected cells m[r] ...
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The bacteriophage P4 alpha gene is the structural gene for bacteriophage P4-induced RNA polymerase.
... As expected, P4 wild-type infected cells produced phage even when shifted early after infection to the nonpermissive temperature; however, P4 wild type is slightly temperature sensitive,[r] ...
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Phenotypic characterization of temperature-sensitive mutants of vaccinia virus with mutations in a 135,000-Mr subunit of the virion-associated DNA-dependent RNA polymerase.
... The fact that all three of the RNA polymerase mutants synthesized DNA at the nonpermissive temperature suggests that once virions are assembled in cells that are infected at the permissi[r] ...
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Mutation in the matrix protein of Newcastle disease virus can result in decreased fusion glycoprotein incorporation into particles and decreased infectivity.
... To determine whether the lower yield of infectious virus, especially at nonpermissive temperature, might be due to the production of fewer virus particles, or possibly to the production [r] ...
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A mutated membrane protein of vesicular stomatitis virus has an abnormal distribution within the infected cell and causes defective budding.
... We find that M proteins of both mutants aggregate in perinuclear regions and are associated with the pellet fraction of infected cells maintained at nonpermissive temperature, whereas at[r] ...
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Protease bypass of temperature-sensitive murine leukemia virus maturation mutants.
... Cells infected with these mutants and maintained at the nonpermissive temperature 39°C release virtually no virions whether measured by assaying the culture media for reverse transcripta[r] ...
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Ultrastructural characterization of an early, nonstructural polypeptide of herpes simplex virus type 1.
... In contrast, in cells infected with tsB2 at the nonpermissive temperature, in which VP175 is overproduced 5 and virus DNA synthesis is not detected 1, staining for the protein in a margi[r] ...
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Characterization of a live, attenuated human parainfluenza type 3 virus candidate vaccine strain.
... the nonpermissive temperature, mRNA synthesis from the P protein gene of cp45 virus was studied by reverse transcription-PCR for comparison with that of the wild-type virus, by a method similar to that ...
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Two small virus-specific polypeptides are produced during infection with Sindbis virus.
... Evidence of this is the fact that cells infected with a certain class of temperature-sensitive mutants produce, at the nonpermissive temperature, no viral structural proteins, but instea[r] ...
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Temperature-sensitive mouse cell factors for strand-specific initiation of poliovirus RNA synthesis.
... These results indicate that human poliovirus receptors on TgSVA and TgSVB cells are functional at the nonpermissive temperature of 40°C and that very early steps of virus infection, incl[r] ...
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Role of a viral membrane polypeptide in strand-specific initiation of poliovirus RNA synthesis.
... We first compared the synthesis of nucleotidyl protein induced by mutant and wild-type CRCs after different times of in vitro incubation at the permissive or nonpermissive temperature an[r] ...
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Adenovirus L1 52- and 55-kilodalton proteins are required for assembly of virions.
... DNA replication and synthesis of late proteins occurred normally in ts369-infected cells at the nonpermissive temperature, but mature virions were not produced.. Rather, capsidlike parti[r] ...
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A temperature-sensitive mutation in a herpes simplex virus type 1 gene required for viral DNA synthesis maps to coordinates 0.609 through 0.614 in UL.
... At the nonpermissive temperature, ts701 i failed to induce the synthesis of viral DNA, ii exhibited a dramatically reduced ability to drive replication of a plasmid containing the herpes[r] ...
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Characterization of a temperature-sensitive defect of enterovirus 70: effect of elevated temperature on in vitro transcription.
... Thus block of single-stranded RNA synthesis and accumulation of RF in viral transcription, as seen in EV70 replication at the nonpermissive temperature, might be a phenomenon rather freq[r] ...
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Morphogenesis of bacteriophage phi 29 of Bacillus subtilis: DNA-gp3 intermediate in in vivo and in vitro assembly.
... To quantify DNA packaging at the nonpermissive temperature of 45°C, cells were infected with susl41241 phage at 30°C and labeled with [3Hlthymidine in the presence of HPUra, an inhibitor[r] ...
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Control of expression of the herpes simplex virus-induced deoxypyrimidine triphosphatase in cells infected with mutants of herpes simplex virus types 1 and 2 and intertypic recombinants.
... Of a series of temperature-sensitive mutants of HSV-2 strain HG52, ts9 and tsl3 failed to induce wild-type levels of the enzyme at nonpermissive temperature; ts9 was the most defective m[r] ...
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Structural analysis of the avian sarcoma virus transforming protein: sites of phosphorylation.
... To determine if the reduced phosphorylation of pp6oSrc in NY68-infected cells at the nonpermissive temperature involved a general inability of the protein to be phosphorylated or some mo[r] ...
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Characterization of herpes simplex virus 2 temperature-sensitive mutants whose lesions map in or near the coding sequences for the major DNA-binding protein.
... In cells infected with certain of these mutants at the nonpermissive temperature, the association of the major DNA-binding protein with the cell nucleus was defective.. In these cells, t[r] ...
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