Northern blot
Sequence diversity of human rotavirus strains investigated by northern blot hybridization analysis.
... To determine whether common sequences of this type could be detected, cDNA was prepared against segment 2 of SAl1 virus and hybridized at low stringency to SAl1, Nebraska calf diarrhea v[r] ...
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Two types of mRNA encoding myosin regulatory light chain in carp fast skeletal muscle differ in their 3' non coding regions and expression patterns following temperature acclimation
... cDNA clones encoding the myosin regulatory light chain (RLC) were isolated from a cDNA library prepared from fast skeletal muscle of the carp Cyprinus carpio L. Two types of cDNA clone encoding carp RLC were found with ...
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Unusual transcripts in La Crosse virus-infected cells and the site for nucleocapsid assembly.
... RESULTS As described in the Introduction, when intracellular nucleocapsid RNA isolated from CsCl density gradients is examined by Northern blot analysis with S genome negativestrand ribo[r] ...
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Expression of connexin genes in the human retina
... [37]. Northern membrane was probed by using corresponding hybridization fragments of mouse Cx36, mouse Cx45 [38] and human Cx59 ...the Northern blot were standardized by hybridization of to a probe ...
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Downregulation of aquaporins 1 and 5 in nasal gland by osmotic stress in ducklings, Anas platyrhynchos: implications for the production of hypertonic fluid
... in northern blot analyses of mRNA isolated from nasal glands of untreated (naïve) or osmotically stressed ducklings (replacement of drinking water with a 1% NaCl solution), we observed a decrease in ...
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Gene structure phylogeny and mutation analysis of RING3 - A novel MHC-encoded gene
... Transcripts of the human and mouse RINGS and ORFX genes (Northern blot analysis. Figures 7.2, 7.S and 7.4 show the results of hybridisation of a human RINGS-specific[r] ...
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Changes in RNA expression pattern during the malignant progression of cottontail rabbit papillomavirus-induced tumors in rabbits.
... DISCUSSION Gross analysis of transcripts encoding early genes by Northern blot, Si nuclease, and primer extension analyses revealed no major quantitative or qualitative differences betwe[r] ...
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Productive infection of cultured human lymphoid cells by adenovirus.
... The presence of adenoviral RNA in lymphoid cells in the course of infection was assayed by Northern blot analysis with the following probes: c131 24, a cDNA clone for the ElA mRNAs; pHin[r] ...
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A custom microarray platform for analysis of microRNA gene expression.pdf
... published northern blot and cloning frequency data for microRNAs that are expressed predominantly in one tissue (Table ...of northern analysis, the expression levels of many microRNAs are at the ...
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SIP1 is a catabolite repression-specific negative regulator of GAL gene expression.
... zyme activity in cells bearing multiple copies of SIP1 reflected reduced transcription of the GAL1 gene. The Northern blot is illustrated in Figure 2A. Densitometric analysis [r] ...
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Experimental liver cirrhosis induced by alcohol and iron
... Northern blot analyses of liver RNA samples showed a pattern of changes similar to that seen in the chemical and histological studies, demonstrating iron-induced potentiation of alcoholi[r] ...
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HOXA10 is expressed in response to sex steroids at the time of implantation in the human endometrium
... expressed in the adult human uterus. To examine expression of HOXA10 during the menstrual cycle, Northern blot analysis and in situ hybridization were performed. Expression of HOXA10 dramatically increased ...
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Enolase and Glycolytic Flux Play a Role in the Regulation of the Glucose Permease Gene RAG1 of Kluyveromyces lactis
... KlENO transcription was examined by a Northern blot analysis. The level of the KlENO transcript is slightly higher (twofold) when the cells are grown on 2% glu- cose (Figure 3B) than when they are ...
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Basic Helix-Loop-Helix Transcription Factor Heterocomplex of Yas1p and Yas2p Regulates Cytochrome P450 Expression in Response to Alkanes in the Yeast Yarrowia lipolytica
... While Northern blot analyses clearly showed that the YAS1 and YAS2 genes are essential for the alkane-dependent induction of ALK1 gene transcription, we also noticed that the amount of ALK1 mRNA in the yas1 ...
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Subcellular Localization and Rolling Circle Replication of Peach Latent Mosaic Viroid: Hallmarks of Group A Viroids
... Quantity of PLMVd strands. To establish the quantity of PLMVd strands (expressed as nanograms of PLMVd per mil- ligram of tissue), we performed dot blot hybridizations. This technique does not differentiate ...
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The 5′ RNA Terminus of Spleen Necrosis Virus Stimulates Translation of Nonviral mRNA
... INSs. Northern blot and RT-PCR analyses indicated that RU5 does not increase the steady-state levels or nuclear export of the luc transcript but rather that the U5 region facilitates efficient polyribosomal ...
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Conventional Protein Kinase C Inhibition Prevents Alpha Interferon-Mediated Hepatitis C Virus Replicon Clearance by Impairing STAT Activation
... (C) Northern blot of time course analysis for IFN- ␣ -mediated HCV replicon clearance in the presence or absence of Go ¨6976 cPKC inhibitor (Go ...by Northern blot, and HCV RNA levels were ...
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DDB1 Stimulates Viral Transcription of Hepatitis B Virus via HBx-Independent Mechanisms
... Southern blot analysis of HBV replication ...Western blot analysis, as ...(B) Northern blot analysis of HBV ...(C) Northern blot analysis of HBV transcripts in shDDB1-treated ...
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Expression of uncoupling protein in skeletal muscle and white fat of obese mice treated with thermogenic beta 3 adrenergic agonist
... and Northern blot analyses in inguinal, mesenteric and ret- roperitoneal white fat pads, and also in gastrocnemius and quadriceps muscles, whereas no signal in saline-treated ...
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Mice deficient in α actinin 4 have severe glomerular disease
... Multiple-tissue Northern blot demon- strated α -actinin-4 expression in a variety of mouse tis- sues, with very strong expression in kidney and liver and strong expression in lung and heart (Figure ...
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