nucleic-acid binding
Dimerization of Coronavirus nsp9 with Diverse Modes Enhances Its Nucleic Acid Binding Affinity
... CoVs encode the open reading frame (ORF) 1a and ORF 1ab polyproteins, which are then processed into 15 to 16 nonstructural proteins (nsp’s) by two virus-encoded proteinases, the papain-like and 3C-like proteinases ...
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Multiple Nucleic Acid Binding Sites and Intrinsic Disorder of Severe Acute Respiratory Syndrome Coronavirus Nucleocapsid Protein: Implications for Ribonucleocapsid Protein Packaging
... of binding to RNA. Constructs containing multiple RNA-binding regions showed Hill coefficients greater than 1, suggesting that the N protein binds to RNA ...multisite nucleic acid ...
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Nucleic Acid Binding by Mason-Pfizer Monkey Virus CA Promotes Virus Assembly and Genome Packaging
... ordered nucleic acid structure bound to the basic RKK region ...the nucleic acid is either absent in the particles or is no longer bound to the RKK ...of nucleic acid ...
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Defining nucleic acid-binding properties of avian retrovirus integrase by deletion analysis.
... USA Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The C28.5K product, which lacks the NH2-terminal 26 amino acids of p32 including the two His residues of the His-Cys[r] ...
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Lysine-functionalized nanodiamonds: synthesis, physiochemical characterization, and nucleic acid binding studies
... (0.05 mm). Dry dimethylformamide (DMF), 4M hydrochloric acid (HCl) (in dioxane) and ethidium bromide solution ( ∼ 1% in water) were obtained from Sigma-Aldrich (Oakville, ON, Canada). Boc-lysine(Boc)-OH, ...
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Structural and antigenic analysis of the nucleic acid-binding proteins of bovine and feline leukemia viruses.
... Reaction of anti-FeLV plO and anti-BLV p12 rabbit sera with the NBPs of various retroviruses after transfer of separated viral proteins from SDSPAGE to DBM paper Reaction with antiserum [r] ...
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Nucleic acid binding and other biomedical properties of artificial oligolysines
... In this work, we demonstrated that AOL is an interesting oli- gocation able to condense RNA and dsDNA, provoking in this latter case a stabilization of the DNA complex. In particular, AOL binds to poly rI and poly I:C, ...
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Molecular insights into DNA interference by CRISPR associated nuclease helicase Cas3
... with nucleic acid binding and trans- location in helicases as revealed by mechanistic studies on the NS3 helicase (38, 39), which is structurally similar to TteCas3, as dis- cussed ...
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Nucleic Acid-Dependent Cross-Linking of the Nucleocapsid Protein of Sindbis Virus
... in nucleic acid binding reaction mixtures and in assembled CLPs further indicated that the observed cross-linking of CP(81–264) was identical to the cross-linking observed in particles, suggesting ...
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Full-Length Hepatitis B Virus Core Protein Packages Viral and Heterologous RNA with Similarly High Levels of Cooperativity
... a nucleic acid-binding domain (amino acids 150 to 183) (11, ...34-residue nucleic acid-binding domain is extremely basic, with 17 arginines, consistent with its ...
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Dynamic expression of miRNAs across immature and adult stages of the malaria mosquito Anopheles stephensi
... protein binding and proteolysis which might be important for egg development that requires massive protein deposition in developing ...processes, nucleic acid binding and signal trans- duction ...
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Morys, Stephan (2018): Optimization of shielding and targeting domains within sequence-defined, cationic carriers for pDNA delivery. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... for nucleic acid binding and ...(HGFR) binding peptide (cmb) [181], that was previously found to mediate tumor-specific gene delivery in vitro as well as in vivo [64, 65], was ...
200
Development of nucleic acid vaccines: use of self-amplifying RNA in lipid nanoparticles
... charged nucleic acids via electrostatic inter- actions, resulting in complexes that offer biocompatibility, low toxicity, and the possibility of the large-scale production required for in vivo clinical ...
11
Week16-01 (Lec) Micro 20 (Intro to Viruses).pptx
... wall; no uncoating of nucleic acid Whole virus is engulfed and uncoated, or virus surface fuses with cell membrane; nucleic acid is released. Synthesis and[r] ...
63
Triplex Forming Oligonucleotides – Tool for Gene Targeting
... efficient binding at inversion site: natural bases, intercalators, analogues that bind in- verted pyrimidine by single hydrogen bond and analogues that bind inverted purine by two hydrogen ...
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A two pronged attack on DNA: Targeting guanine quadruplexes with nonplanar porphyrins and DNA binding small molecules
... Moreover, the large planar surface of a terminal GQ has led to the development of drug candidate families based on heteroaromatic systems which complement the GQ platform [2]. As mentioned previously, a common feature of ...
6
Next-Generation Nucleic Acid Aptamers with Two-Base-Modified Nucleotides Have Improved Binding Affinity and Potency
... different binding sites, as is the case with multivalent aptamer isolation (MAISELEX) and array-based discovery platform for multivalent aptamers (AD-MAP) [11,12] ...
5
On nucleic acid photochemistry
... In order to locate the position of thymine and thymine photodimer on the strip of paper, a strip of paper containing just carrier thymine and thymine dimer was chromatographed adjacent t[r] ...
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Binding properties of avian myeloblastosis virus DNA polymerases to nucleic acid affinity columns.
... Although template-free murine sarcoma-leukemia virus DNA polymerase bound to oligodT-cellulose at 0.06 M KCI with the main peak of activity eluting at 0.2 M KCI, approximately 20% of the[r] ...
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Functional nucleic acid probes and uses thereof
... functional nucleic acid probes, and methods of using functional nucleic acid probes, for binding a target to carry out a desired ...functional nucleic acid, at least one ...
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