Paired-end reads
Differentially expressed genes from RNASeq and functional enrichment results are affected by the choice of single end versus paired end reads and stranded versus non stranded protocols
... or paired-end reads in gene expression ana- lysis. Paired-end data was also used to explore the differ- ence that a strand-specific protocol can make compared to a non-strand-specific ...
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Paired-end mappability of transposable elements in the human genome
... simulate paired-end reads, we generated all possible 200-mers, 242-mers, and 300-mers from hg38 using ...two paired-end “reads,” (100 bp, 90 bp, and 100 bp respectively) and ...
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Uncovering hidden variation in polyploid wheat
... 100-bp paired-end reads per sample by using methods described in SI Appendix, Method S2 and Table ...mapped reads, we supplemented the reference wheat genome se- quence (11) with de novo ...
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Comparison of Whole Genome Sequencing Methods for Analysis of Three Methicillin Resistant Staphylococcus aureus Outbreaks
... Based on the three estimated probability distributions, the Bayes factor approach was used to determine the cutoff values for classification of isolates. Given a model selection problem in which we had to choose between ...
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Comparison of assembly algorithms for improving rate of metatranscriptomic functional annotation
... of paired-end reads. The paired-end reads allowed an evaluation of a dedicated metatranscriptomic assembly algorithm, IDBA-MT [20], as it is compatible only with ...
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SHI7 Is a Self-Learning Pipeline for Multipurpose Short-Read DNA Quality Control
... merge paired-end reads to- gether into longer or higher-quality ...Furthermore, end users may not know all of the relevant infor- mation about how their data were generated, such as the ...
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Sequence-Based Detection and Breakpoint Assembly of Polymorphic Inversions
... single- end reads, they are not suitable for direct comparison via our method, which relies on independently mapped paired- end ...using paired-end ...
7
A cost effective and universal strategy for complete prokaryotic genomic sequencing proposed by computer simulation
... single end and one run of paired end reads, 90% of the 100 genome assemblies are less than 10 scaffolds and 95% of 100 genomes assemblies are less than 150 contigs (400 bp read ...
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Konnector v2.0: pseudo-long reads from paired-end sequencing data
... Working with a maximum of 120 GB available memory on any single machine, Konnector and Konnector v2.0 were the only tools that could be run on the largest of the four data sets in Table 2 (H. sapiens). One of the main ...
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Multilocus Sequence Typing of Total Genome Sequenced Bacteria
... sequence reads, along with the sequencing platforms ...generating paired-end reads by TGen (United ...generating paired-end reads by Source BioScience (United Kingdom), ...
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A radiographic analysis of tooth morphology following the use of a novel cyclical force device in orthodontics
... Furthermore, there have been conflicting discussions of what is considered to be clinically significant root resorption. Some authors have stated that root resorp- tions in excess of 1/3 of root length were significant ...
5
IMP: a pipeline for reproducible reference independent integrated metagenomic and metatranscriptomic analyses
... Assessments based on real datasets demonstrate comparable performance between IMP and IMP- megahit while both outperform MetAMOS_MGMT and MOCAT_MGMT in all measures (Fig. 4a–c). The ground truth assessment using the SM ...
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Under what conditions do end users perceive high value of HR Shared Service Centers?: A Service Dominant logic perspective
... the end-user’s value perception to be high, and thus value follows from the interrelationship between the three sub-dimensions (Reed et ...the end-user; in all cases, high value perception or no high value ...
61
Identification and analysis of miRNAs in human breast cancer and teratoma samples using deep sequencing
... The negative sets were made by random sampling of pre- cursors from three different sequence sets: A) the full human genome (hg18, March 06 assembly). B) a ncRNA set made by concatenating the non-miRNA sequences from the ...
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League of Women Voters V. State: The Rejection of Public and Private Hybridity Within Washington State Schools
... On September 4, 2015, in a 6-3 decision in League of Women Voters of Washington v, State, the Washington State Supreme Court became the first in the nation to deem charter schools funded by taxpayers unconstitu- tional. ...
29
Smart Fusion Based cSoC for Wireless Sensor Network for Agricultural Applications
... The Analog Front End (AFE) of programmable analog block reads the analog signal coming from sensors. Deploying on-chip ADC in ACE, it digitizes the signal to 10-bit resolution[r] ...
8
Tracing mother-infant transmission of bacteriophages by means of a novel analytical tool for shotgun metagenomic datasets: METAnnotatorX
... million reads for the meconium sam- ples and 25 million reads for fecal samples of ...148,797,588 reads, ranging from 238,288 to 34,105,775 reads (Additional file 1: Table ...eukaryotic ...
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40124932fd563a4f53e59a813f8eb1aecc3febfc.pdf
... During a typical genome assembly process, overlap- ping reads are first used to build contigs, then contigs are connected into larger scaffolds using order and ori- entation information from mate–pair ...
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A Pipeline for Markers Selection Using Restriction Site Associated DNA Sequencing (RADSeq)
... of reads. The highest number of reads and mapped reads are detected in the same sample of next generation sequences ...of reads is indicated, including mapped and unmapped ...of reads ...
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CYTOGENETICS AND PHYLOGENY OF OENOTHERA ARGILLICOLA MACKENZ
... The absence of balanced lethals, the presence of mainly paired chromosomes, of large, open-pollinated flowers and of segmental end arrange- ments which are close to th[r] ...
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