Pb(CH3COO)2
The Effect of the PB2 Mutation 627K on Highly Pathogenic H5N1 Avian Influenza Virus Is Dependent on the Virus Lineage
... revert PB2 to K627E. When the A1869G mutation was engi- neered into the PB2 gene, the 628R mutation was also selected to various degrees during independent experiments ...avian PB2 in cells or mice, ...
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A Replication-Incompetent PB2-Knockout Influenza A Virus Vaccine Vector
... cubated with 2-fold serial dilutions of receptor-destroying enzyme- treated sera for 30 min at 33°C, and the mixtures were added to confluent MDCK cells on 96-well microplates to determine the neutralizing activity. IFA ...
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The PB2 Subunit of the Influenza A Virus RNA Polymerase Is Imported into the Mitochondrial Matrix
... chondrial PB2 for virus growth in cell culture, we infected wild- type MEFs with recombinant A/WSN/33 (H1N1) influenza A viruses which encode either a wild-type mitochondrial N9-PB2 (N9-WSN) or a mutant ...
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Giant Catalytic DNA Particles for Simple and Intuitive Detection of Pb2+
... This is probably due to the fact that the bulk of cleaved substrate strands were separated from the 17E DzMPs because of the catalytic activity of DNAzyme in the presence of Pb2+.. For a[r] ...
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Mammalian Adaptation in the PB2 Gene of Avian H5N1 Influenza Virus
... of PB2 amplicons from the respiratory tract tissues of mice infected with the VN1203 virus retained K at PB2-627 over the 7 days ...to PB2-627K was apparent by day 6 ...at PB2-627) of an avian ...
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The influenza A virus PB2 polymerase subunit is required for the replication of viral RNA.
... Deficient systems in which any single viral element was lack- ing were reconstituted in vivo. Poly(A) 1 and poly(A) 2 RNAs were isolated from these cultures and were analyzed by RNase protection assay as indicated above. ...
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Virulence Determinants in the PB2 Gene of a Mouse-Adapted H9N2 Virus
... FIG 2 Viral polymerase activity and growth kinetics. (A) Polymerase activities of the viral RNP complex in a minigenome assay. The luciferase reporter plasmid p-Luci and internal control plasmid Renilla were transfected ...
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Role of the PB2 627 Domain in Influenza A Virus Polymerase Function
... the PB2 627 domain is also required for activity in avian cells, RNPs were reconstituted in avian DF-1 ...of PB2 has been implicated in RNP assembly, putatively by recruiting or interacting with NP ...
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Sequential addition of temperature-sensitive missense mutations into the PB2 gene of influenza A transfectant viruses can effect an increase in temperature sensitivity and attenuation and permits the rational design of a genetically engineered live influenza A virus vaccine.
... double-mutant PB2 transfectant viruses for stability of the ts phenotype, and two of the three double-mutant PB2 viruses exhibited complete sta- bility of this phenotype even after prolonged replication in ...
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Cd2+ and Pb2+ Induced Structural, Functional and Compositional Changes in the Liver and Muscle Tissue of Crucian Carp (Carassius auratus gibelio): an FT-IR Study
... Cd2+ and Pb2+ Induced Structural, Functional and Compositional Changes in The Liver and Muscle Tissue of Crucian Carp (Carassius auratus.. gibelio): an FT-IR Study.[r] ...
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Introduction of Virulence Markers in PB2 of Pandemic Swine-Origin Influenza Virus Does Not Result in Enhanced Virulence or Transmission
... the PB2 gene, E627K, E677G, and D701N, on the activity of the influenza virus polymerase ...virus PB2, PB1, PA, and NP proteins, negative-sense vRNA is copied to positive-sense cRNA and mRNA, and the ...
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ITS and pB2 5 gene expression of Naegleria fowleri in drug resistance
... The purpose of this study was to examine the effects of drugs amphotericin B, artesunate, azithromycin, voriconazole, chlorpromazine, fluconazole and gentamicin sulphate on ITS and pB2.3[r] ...
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Simultaneously Detection of Pb2+ and Hg2+ Using Electrochemically Reduced Graphene Oxide
... The calibration curve of stripping peak current versus the concentration of Pb2+ a and Hg2+ b under the optimal conditions concentration of GO: 0.1 mg/mL; deposition potential: −1.0 V; a[r] ...
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Reinvestigation of the Pb2[B5O9]Br structure based on single crystal data
... The PbÐO distances in each polyhedron divide into two groups. Rather strong Pb±O bonds, corresponding to the criterion of the sum of ionic radii, lie between 2.497 (13) and 2.686 (9) AÊ at Pb1, and between 2.475 (10) and ...
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The Temperature-Sensitive and Attenuation Phenotypes Conferred by Mutations in the Influenza Virus PB2, PB1, and NP Genes Are Influenced by the Species of Origin of the PB2 Gene in Reassortant Viruses Derived from Influenza A/California/07/2009 and A/WSN/33 Viruses
... internal PB2, PB1, PA, NP, M, and NS gene segments (backbone) derived from a human influenza A/Ann Arbor/6/60 (H2N2) virus (AA) that is cold adapted (ca), temperature sensitive (ts), and attenuated (att) ...
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PB2 Residue 158 Is a Pathogenic Determinant of Pandemic H1N1 and H5 Influenza A Viruses in Mice
... identified PB2-E158G/A, along with eight other amino acid changes in PA (L295P), NP (D101G and H289Y), and HA (K119N, G155E, S183P, R221K, and D222G), as putative pathogenic determinants that were iden- tified in ...
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Ultrasensitive DNAzyme-based electrochemical biosensor for Pb2+ based on FcHT-mediated biocatalytic amplification
... 3.3 Detection of Pb2+ with electrochemical biosensor To obtain high sensitivity for Pb2+ detection, we optimized some experimental parameters such as H2O2 concentration and reaction time[r] ...
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Association of the Influenza Virus RNA Polymerase Subunit PB2 with the Host Chaperonin CCT
... with PB2, we also confirmed the association of the PB2 protein with the previously identified binding partners Hsp90 and Hsp70 (7, 22, 36, ...the PB2 protein, suggesting that Hsp90 acts as a ...
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Evolution of influenza A virus PB2 genes: implications for evolution of the ribonucleoprotein complex and origin of human influenza A virus.
... * 4893 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Phylogenetic analysis of 20 influenza A virus PB2 genes showed that PB2 genes have evolved into the following fou[r] ...
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PB2-588I Enhances 2009 H1N1 Pandemic Influenza Virus Virulence by Increasing Viral Replication and Exacerbating PB2 Inhibition of Beta Interferon Expression
... Previous studies have shown that the NS1 protein of the 1918 virus pandemic strain mediated the delay of type I interferon in- duction, which contributed to the virulence of this strain (36). The decreased ability of the ...
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