Plasma DNA
Quantitation and characterization of plasma DNA in normals and patients with systemic lupus erythematosus
... vitro DNA labeling technique of nick translation on purified plasma DNA, we have estimated the plasma DNA concentration in three normal individuals to be 266 +/- 57 ng/ml (mean +/- ...
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Circulating DNA in systemic lupus erythematosus Isolation and characterization
... (SLE). Plasma from 10 such patients was subjected to a DNA isolation procedure that used a combination of proteolysis, phenol extraction, and hydroxylapatite adsorption and elution in the presence of ...the ...
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Human cytomegalovirus (CMV) DNA in plasma reflects quantity of CMV DNA present in leukocytes
... with plasma or serum DNA in a single sample has been reported by some investigators (1, 14, 21), but others have not found this rela- tionship ...CMV DNA levels in plasma among laboratories ...
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Endogenous circulating DNA in systemic lupus erythematosus Occurrence as multimeric complexes bound to histone
... (SLE) plasma DNA even though it is the presumed precursor of DNA-containing immune complexes, thought to play a central role in lupus ...glomerulonephritis. DNA purified from SLE plasma ...
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Free DNA in serum and plasma from normal adults
... Normal plasma and serum samples were examined by four assays for DNA that were newly modified to enhance their specificity and/or ...sensitivity. Plasma DNA was undetectable by all four ...
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Plasma nuclear and mitochondrial DNA levels as predictors of outcome in severe sepsis patients in the emergency room
... higher plasma mitochondrial DNA level on admission is a powerful biomarker for fatality in severe septic patients, this study has several ...First, plasma nuclear and mitochondrial DNA levels ...
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Noninvasive detection of tumor-associated mutations from circulating cell-free DNA in hepatocellular carcinoma patients by targeted deep sequencing
... in plasma could be used to predict clinical outcome in the patients with HCC who received surgical ...in plasma DNA was lost to ...in plasma DNA was 89 days (range, 34 to 299 days), ...
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Methylation of the imprinted GNAS1 gene in cell free plasma DNA : equal steady state quantities of methylated and unmethylated DNA in plasma
... cfp DNA from the circuation are not well understood processes [2], it is likely that our finding of equal representation in the steady state can be seen as indicating equal rates of clearance of the two allelic ...
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Plasma cell free DNA in patients needing mechanical ventilation
... of plasma cell-free DNA were higher in non-survivors than in sur- ...raises plasma cell-free DNA concentra- tions even when the disease process is not potentially ...study plasma ...
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The dynamic range of circulating tumor DNA in metastatic breast cancer
... 74 plasma DNA samples from 58 patients with metastasized breast cancer were analyzed with a microfluidic device to determine the plasma DNA size distribution and copy number changes in the ...
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Clinical Significance of Low Cytomegalovirus DNA Levels in Human Plasma
... CMV DNA copy numbers below 100/ml, though the clini- cal significance of such values remained unclear (3, ...CMV DNA down to 20 copies/ml of patient plasma ...CMV DNA detectable by their new ...
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Comparison of Quantitative and Qualitative PCR Assays for Cytomegalovirus DNA in Plasma
... CMV DNA in clinical ...CMV DNA PCR assays and have compared the quantitative assay to the in-house-developed assay that was used to determine the clinical significance of CMV viral load in patients with ...
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Plasma mitochondrial DNA and metabolomic alterations in severe critical illness
... biorepository plasma samples collected from 73 adults with systemic inflammatory response syndrome or sepsis at a single academic medical ...to plasma NADH dehydrogenase 1 (ND1) mtDNA levels in critical ...
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Detection of Marek's Disease Virus DNA in Chicken but Not in Human Plasma
... MDV DNA but were unable to confirm a role for MDV in the pathogenesis of ...MDV DNA in 20% of 202 human serum samples, regardless of whether the individuals were exposed to ...MDV DNA in chicken ...
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A New Biomarker for Hepatocellular Damage: Plasma Cell Free DNA
... generally used by clinicians to assess the severity of liver injury. Aminotransferases and bilirubin are sensitive indicators of hepatocellular damage. The highest elevations of the ami- notransferases level occur in ...
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A Modified Extraction Method of Circulating Free DNA for Epidermal Growth Factor Receptor Mutation Analysis
... including 5 uLof template DNA, 16 uL of reaction buffer mix, 0.6 uL of Taq polymerase and 3.4 uL water. Real-time PCR was carried out by using MX3005P real-time PCR machine (Stratagene, La Jolla, CA, USA) under ...
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Optimization of Brucella abortus Protocols for Downstream Molecular Applications
... inoculation volume of 1 ml. Tissues were incubated in a humidified incubator at 39°C for 24 h prior to homogenization. Tissues were incubated for 24 h to allow the bacterial cells to infect the tissues in an intracellular ...
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On the Death Rate of Abortively Infected Cells: Estimation from Simian-Human Immunodeficiency Virus Infection
... SHIV DNA in PBMCs by a DSP ...Genomic DNA was extracted from 200- l aliquots using a QIAamp DNA and Blood minikit (Qiagen) and resus- pended in 100 l of elution ...SHIV DNA was quantified ...
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THE POSSIBLE INVOLVEMENT OF EPSTEIN BARR VIRUS IN THE ETIOLOGY OF LEUKEMIA
... that plasma cell-free EBV DNA is a highly specific marker for a screening and diagnosis of EBV-associated ...of plasma cell-free EBV DNA are warranted features in childhood ...
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Simian Retrovirus 4 Induces Lethal Acute Thrombocytopenia in Japanese Macaques
... genomic DNA was extracted from various tissues of Japanese macaques using a QIAamp DNA minikit (Qia- ...the DNA concentration, standard curves were generated for each gene by serial dilution of the ...
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