Primordial germ cell
Drosophila primordial germ cell migration requires epithelial remodeling of the endoderm
... moe::nos 3⬘UTR-expressing mothers and carry zygotic copies of this transgene. (A) Endodermal epithelium is outlined in yellow. Arrow highlights a migrating primordial germ cell (PGC). Still images ...
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DNA methylation is a primary mechanism for silencing postmigratory primordial germ cell genes in both germ cell and somatic cell lineages
... postmigratory primordial germ cells coincides with erasure of genomic imprints and reactivation of the inactive X chromosome, as well as ongoing germ cell differentiation ...in germ ...
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A novel role for sox7 in Xenopus early primordial germ cell development: mining the PGC transcriptome
... flow cell of the Illumina cBot cluster ...Flow Cell v4 with 100-bp paired-end reads plus index read using the Illumina HiSeq SBS Kit ...laevis primordial germ cell RNA and three from ...
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Primordial germ cell development: is the urodele pattern closer to mammals than to anurans?
... Int I I) ,' Riol ~O 653 659 (19961 653 Primordial germ cell development is the urodele pattern closer to mammals than to anurans? MASAMI WAKAHARA* Biological Sciences, Graduate School of Science Hokka[.] ...
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Cellular and molecular aspects of mouse primordial germ cell migration and proliferation in culture
... Int I Un BioI 36 205 213 (1992) Review Cellular and molecular aspects of mouse primordial germ cell migration and proliferation in culture MASSIMO DE FELlCI*, SUSANNA DOLCI and MAURIZIO PESCE Departme[.] ...
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Primordial germ cell proliferation in the salamander Pleurodeles waltl: genetic control before gonadal differentiation
... Inl / De\' Bio1 33 477 4R5 (19R9) 477 Original Article Primordial germ cell proliferation in the salamander Pleurodeles walt/ genetic control before gonadal differentiation CHRISTIAN DOURNON* CHRISTIA[.] ...
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Silencing markers are retained on pericentric heterochromatin during murine primordial germ cell development
... We first reanalysed the reported dynamics of H3K9me3 [27] in PGCs of E10.5–E13.5 mouse embryos. For this, we used a fluorescent immunohistochemical approach. Since fixation conditions may influence epitope avail- ...
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SETDB1 is essential for mouse primordial germ cell fate determination by ensuring BMP signaling
... In this study, we focused on another candidate, SETDB1, which catalyzes the tri-methylation of lysine 9 (K9) of histone H3 and was identified by RNAi screening. Methylated K9 of histone H3 is a repressive epigenetic ...
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An Initial Investigation of an Alternative Model to Study rat Primordial Germ Cell Epigenetic Reprogramming
... which germ cells go through is one of the most interesting events of their pre-natal ...for germ cell differentiation [21] and maybe to confer the de- velopmental potential to the future ...
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A possible role of Reproductive homeobox 6 in primordial germ cell differentiation
... placenta-specific homeobox, Gpbox, or Placenta-specific homeo- box 2, Psx2; Han et al., 2000; Takasaki et al., 2000). Comparable levels of Rhox9 transcripts were initially detected in the urogenital ridge at the onset of ...
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Primordial germ cell migration
... 2003), cell-cell adhesion (E-cadherin and foi) (Bendel-Stenzel et ...mouse, germ cell adhesion may be controlled via a PGC intrinsic clock as PGCs in culture cluster and become non-motile at ...
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SMAD2 and p38 signaling pathways act in concert to determine XY primordial germ cell fate in mice
... because the suppression of RA signaling by a retinoic acid receptor (RAR) inhibitor completely recovered Nanos2 expression. Therefore, p38 signaling is required for the suppression of RA signaling. Given that Cyp26b1 is ...
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Disruption of genital ridge development causes aberrant primordial germ cell proliferation but does not affect their directional migration
... induced cell morphological changes characteristic of epithelial- mesenchymal transition, accompanied by a complete loss of the epithelial markers E-cadherin and a significant ele- vation of mesenchymal markers ...
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Leapfrogging: primordial germ cell transplantation permits recovery of CRISPR/Cas9 induced mutations in essential genes
... Efficient CRISPR/Cas9 mutagenesis in PGC transplants Since we wished to combine CRISPR/Cas9 mutagenesis with PGC transplantation, we sought an indirect assay for determining the efficacy of mutagenesis in the ...
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Primordial germ cell biology at the beginning of the XXI Century
... This is certainly the topic in which the most impressive ad- vances have recently occurred (for a review, see McLaren and Lawson, 2005; Hayashi et al., 2007). Briefly, we now know that at 5-6 dpc, the pluripotent ...
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Primordial germ cell-mediated transgenesis and genome editing in birds
... PGC cell-surface markers, PGCs were isolated using a density gradient-dependent centrifu- gation method [39, ...quail germ cell-specific marker (QCR1) in quail, it is pos- sible to collect highly ...
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Nodal induces sequential restriction of germ cell factors during primordial germ cell specification
... its germ line by inductive events and that this mechanism requires Nodal signaling for the sequential restriction of cells that retain germ cell ...inductive germ cell specification in ...
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How to make a primordial germ cell
... promotes cell identity (Graham et ...inner cell mass (ICM) and embryonic stem cells (ESCs) with respect to the expression of pluripotency genes, PGCs are unique because they exhibit the imprinting cycle and ...
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Chicken primordial germ cell motility in response to stem cell factor sensing
... The cell mixture was subsequently loaded on the observation area in the middle channel, which was a gap connected to the two parallel reservoirs on either ...
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Regulation of primordial germ cell development in the mouse
... STO cell feeder layers (De Felici, unpublished ...of cell feeder layers producing mem- brane bound SCF to support survival/proliferation of PGCs in vitro (Dolci et ...
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