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Prokaryotic Expression

Construction and use of a prokaryotic expression system for Helicobacter pylori AhpC

Construction and use of a prokaryotic expression system for Helicobacter pylori AhpC

... blot, indicating that the fusion protein had high immu- noreactivity. However, we could not explain presence of lower intensity bands other than that of the right sized band, both in SDS-PAGE (Figure 3B) and their subse- ...

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CLONING, PROKARYOTIC EXPRESSION AND PROTEIN PURIFICATION OF SUGARCANE ENDOGENOUS AZOTOBACTER KLEBSIELLA VARIICOLA DX120E NIFK GENE

CLONING, PROKARYOTIC EXPRESSION AND PROTEIN PURIFICATION OF SUGARCANE ENDOGENOUS AZOTOBACTER KLEBSIELLA VARIICOLA DX120E NIFK GENE

... methods. Prokaryotic expression vector pET30a (+) was used to construct the recombinant expression vector ...gene expression at 28 o C. The fusion protein expression was ...

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Prokaryotic expression, purification of a novel candidate tumor suppressor gene FUS1 and characterization of its polyclonal antibodies

Prokaryotic expression, purification of a novel candidate tumor suppressor gene FUS1 and characterization of its polyclonal antibodies

... FUS1 is a novel candidate tumor suppressor gene identified in human chromosome 3p21.3. Its expres- sion showed significantly reduction or even loss in lung cancer and other types of cancers. In order to further ...

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Prokaryotic Expression of Influenza A virus Nucleoprotein Fused to Mycobacterial Heat Shock Protein70

Prokaryotic Expression of Influenza A virus Nucleoprotein Fused to Mycobacterial Heat Shock Protein70

... Nucleoprotein of influenza virus, which has conservatory sequence and immunodominant epitopes being good CTL targets for releasing T-cell cross protections, is appropriate candidate for making universal vaccine (14). NP ...

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cDNA Cloning, Prokaryotic Expression of Two Splicing Products of mLRG, a Mouse Gene of Lipopolysaccharide Response

cDNA Cloning, Prokaryotic Expression of Two Splicing Products of mLRG, a Mouse Gene of Lipopolysaccharide Response

... the prokaryotic expression vector pTAT so as to construct the recombinant expression vector pTAT-MLRGW and ...and prokaryotic protein expressions are of help in the further study of this ...

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Prokaryotic expression and characterization of the heterodimeric construction of ZnT8 and its application for autoantibodies detection in diabetes mellitus

Prokaryotic expression and characterization of the heterodimeric construction of ZnT8 and its application for autoantibodies detection in diabetes mellitus

... Unless otherwise indicated, standard molecular biol- ogy protocols were used according to Sambrook et al. [30]. Restriction enzymes, Pfu polymerase and T4 ligase were from Promega (Madison, WI, USA). The coding sequence ...

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Transcription phase, protein characteristics of DEV UL45 and prokaryotic expression, antibody preparation of the UL45 des transmembrane domain

Transcription phase, protein characteristics of DEV UL45 and prokaryotic expression, antibody preparation of the UL45 des transmembrane domain

... its expression level is relative constancy in cells) were UL45 F (5’- CATGGAGTTGGGTGTGCT -3’) and UL45 R (5’-ACGCTGTAGTCGGTATCG -3’), b-actin F (5’-CCGGGCATCGCTGACA-3’) and b-actin R (5’- ...

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Prokaryotic Expression of the Oligopeptide Transporter hPepT2(560-663)

Prokaryotic Expression of the Oligopeptide Transporter hPepT2(560-663)

... bacterial expression of hPepT2(560-663) was detected by SDS-PAGE electrophoresis, the optimum expression conditions (at 37°) were identified as: 3 h and a working concentration of ...

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Prokaryotic expression of a VP1 polypeptide antigen for diagnosis by a human parvovirus B19 antibody enzyme immunoassay

Prokaryotic expression of a VP1 polypeptide antigen for diagnosis by a human parvovirus B19 antibody enzyme immunoassay

... To produce parvovirus B19 antigen for diagnostic purposes, partially overlapping segments covering the genes encoding the viral structural proteins VP1 and VP2 were cloned into expressio[r] ...

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Original Article Prokaryotic expression of partial E gene of DENV strain and its effect on HUVEC permeability

Original Article Prokaryotic expression of partial E gene of DENV strain and its effect on HUVEC permeability

... We isolated the virus (M strain, the same below) from Aedes albopictus in the nature of Ma Wei town, Dushan County, Guizhou Province of Aedes albopictus in 2002. The virus was identi- fied as DENV-2 by indirect ...

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Original Article Purification and characterization of Toxoplasma gondii immune mapped protein-1

Original Article Purification and characterization of Toxoplasma gondii immune mapped protein-1

... the prokaryotic expression system, and to identify the purified ...the expression vector pET28b and the recombinant pET28b-TgIMP1 was identified by double digestion and ...

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Molecular and catalytic properties of fungal extracellular cellobiose dehydrogenase produced in prokaryotic and eukaryotic expression systems

Molecular and catalytic properties of fungal extracellular cellobiose dehydrogenase produced in prokaryotic and eukaryotic expression systems

... This study compared the suitability of four different expression systems for the production of CDH. We eval- uated the titer of secreted CDH, the purification proce- dure and yield together with the molecular and ...

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Establishment of antigen capture ELISA detection method for H9 subtype avian influenza virus HA protein

Establishment of antigen capture ELISA detection method for H9 subtype avian influenza virus HA protein

... the expression of HA protein, it is important to choose the expression system, the Escherichia coli expression system has the advantages of low cost, convenient, simple operation, cultivating a large ...

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Characterization of Schistosoma japonicum CP1412 protein as a novel member of the ribonuclease T2 molecule family with immune regulatory function

Characterization of Schistosoma japonicum CP1412 protein as a novel member of the ribonuclease T2 molecule family with immune regulatory function

... a prokaryotic expression system and demonstrated to have ribonuclease activity, which could inhibit DC cell activa- tion, promote M2 macrophage differentiation, increase the number of Treg cells and drive ...

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Challenging for Expression Bovine Rotavirus (RF Strain) Full-Length VP7 Protein in Prokaryotic System

Challenging for Expression Bovine Rotavirus (RF Strain) Full-Length VP7 Protein in Prokaryotic System

... the prokaryotic expression vector ...the expression of pET32a-VP7 synthetic was weak at 30° C in luria bertani ...in prokaryotic system ...

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Over Expression of Influenza Virus M2 Protein in Prokaryotic System

Over Expression of Influenza Virus M2 Protein in Prokaryotic System

... and expression of influenza A virus M2 protein as a fusion with 6-His tag in Escherichia coli BL21 ...the prokaryotic expression vector pET28a. The expression of M2 protein was induced by IPTG ...

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Development of a dual-expression vector facilitated with selection-free PCR recombination cloning strategy

Development of a dual-expression vector facilitated with selection-free PCR recombination cloning strategy

... prokaryotic expression of the candidate gene is usually necessary to prepare antiserum for verification of the antigenicity of the candidate gene or to prepare antigen for monitoring the efficiency of the ...

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Defective cystathionine beta synthase regulation by S adenosylmethionine in a partially pyridoxine responsive homocystinuria patient

Defective cystathionine beta synthase regulation by S adenosylmethionine in a partially pyridoxine responsive homocystinuria patient

... four different methods. First, we found no other mutations in the coding region of the CBS gene, which could be responsible for the severely elevated homocysteine and methionine levels. Second, the mutation was absent in ...

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The Expanded Universe of Prokaryotic Argonaute Proteins

The Expanded Universe of Prokaryotic Argonaute Proteins

... ABSTRACT Members of the ancient family of Argonaute (Ago) proteins are present in all domains of life. The common feature of Ago proteins is the ability to bind small nucleic acid guides and use them for sequence-specific ...

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Precise Circadian Clocks in Prokaryotic Cyanobacteria

Precise Circadian Clocks in Prokaryotic Cyanobacteria

... As mentioned above, kaiC appears to be an internally duplicated version of a recA/dnaB-like gene; it has two parts that are very similar to each other (Iwasaki et al., 1999). In each half of the kaiC gene, there is a ...

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