Protein quantification using label-free system
Tools for label-free peptide quantification
... ions eluting from the LC column. Peptides elute over time from the LC column, get ionized and in- jected into the mass spectrometer. The mass spectrometer takes now measurements in regular, small time intervals thereby ...
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Label-free, simultaneous quantification of starch, protein and triacylglycerol in single microalgal cells
... Results: Here, we established a method based on single-cell Raman spectra (SCRS) that simultaneously quantifies the contents of starch, protein, triacylglycerol (TAG) and lipid unsaturation degree in individual ...
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Automated Selected Reaction Monitoring Software for Accurate Label-Free Protein Quantification
... virulence. 27 Growing S. pyogenes with plasma changes the proteome homeostasis of the bacterium but also represents a vastly di fferent sample as there are a substantial amount of plasma proteins present. Nevertheless, ...
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Visualization and label-free quantification of microfluidic mixing using quantitative phase imaging
... *Corresponding author: [email protected] Received 15 May 2017; revised 6 July 2017; accepted 7 July 2017; posted 10 July 2017 (Doc. ID 296024); published 31 July 2017 Microfluidic mixing plays a key role in various ...
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New label-free methods for protein relative quantification applied to the investigation of an animal model of Huntington Disease
... by using a linear gra- dient of eluent B (95% acetonitrile LC-MS grade (Sigma Aldrich, ...relative quantification of the specific proteins. For each protein, at least two proto- typic peptides were ...
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Robust summarization and inference in proteome-wide label-free quantification
... given protein in each sample, resulting in a bias reduction and a better uncertainty estimation on the fold change ...given protein in the same ...provide protein summaries for each sample, which are ...
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Label-free quantitative proteomics trends for protein–protein interactions
... robust quantification approach, a multisite validation of SRM assays was undertaken by the CPTAC group [56] , introducing guidelines and a framework for SRM-based measurements and highlighting the possibility of ...
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Mapping in vivo target interaction profiles of covalent inhibitors using chemical proteomics with label-free quantification
... Whereas quantification of protein activity in our previ- ous protocol was performed by dimethyl labeling 12 , we now describe a label-free quantification protocol 17 with the use of ...
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Quantification of Protein Expression on an Immunofluorescence Section by Using the MetaMorph Image Analysis System
... The quantification of immunostaining by means of digital image analysis by using Leica MetaMorph AF ...of protein expression revealed by immunohistochemistry on normal or pathological tissue ...by ...
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A label-free electrochemical protein sensor of perchloric acid doped polyaniline
... During the polymerization of polyaniline in the presence of PA was observed a short and thick rod like structure (Tahir et al., 2005) which was similar to one observed by (Abe et al. 1989). In general, the polyaniline ...
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Silicon Photonic Biosensors Using Label-Free Detection
... for label-free detection, are exhibited and ...delivery system, optical input and readout ...advanced system-level CMOS-chip packaging examples are presented, indicating the commercialization ...
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Label-free mass spectrometry-based quantification of hemagglutinin and neuraminidase in influenza virus preparations and vaccines
... of protein type and ...any protein in the mixture can be ...for protein analysis over conventional LC ⁄ MS ⁄ MS approaches, as it utilizes parallel, multiplex fragmentation where all peptide ...
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Detecting of Benzo[a]pyrene Using a Label-free Amperometric Immunosensor
... 3e), the semi-circle diameter in the impedance spectrum increased gradually. This was attributed to the immobilization of LC and NG on the NG/PB/GCE film that hindered the access of the redox probe to the electrode. With ...
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Label-free breast histopathology using quantitative phase imaging
... Having demonstrated that our feature set has the ability to detect malignancy on stained tissue biopsies, we next applied our model to a more diagnostically challenging problem. Stratification of benign lesions in breast ...
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Label-free and washing-free alkaline phosphatase assay using a personal glucose meter
... ALP system could be utilized to reliably determine the ALP activity in human blood and applied to the assay of ALP contained in the whole blood samples obtained by finger prick, ensuring its future clinical ...
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Identification of potential protein biomarkers for early detection of pregnancy in cow urine using 2D DIGE and label free quantitation
... create protein–protein interaction network for iden- tified urinary proteins, offline software tool Cytoscape was used along with plug-in ...and protein kinase ...
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Label Free C-reactive Protein Detection Based on An Electrochemical Sensor for Clinical Application
... system above, we proposed two approaches to CRP screening in blood serum. In terms of the former capacity, the controllable dilution in PBST allows for the in situ detection of CRP spiked blood serum, as indicated ...
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Quantification of malaria parasite release from infected erythrocytes: inhibition by protein free media
... Method and results: To quantify parasite release process, human erythrocytves infected with Plasmodium falciparum were injected into sealed chambers at optimal density, where they progressed through the end of the ...
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Label-free Detection of Single Molecule Using Microtoroid Optical Resonators
... Abstract Being able to detect a single molecule without the use of labels has been a long standing goal of bioengineers and physicists. This would simplify applications ranging from single molecular binding studies to ...
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Take it personal! Genetic differences in G protein-coupled receptors as studied with label-free technology
... sensitive label-free technology, the ...LCLs. Using the CB 2 R as a prototypical GPCR, we were able to show that receptor activation by an agonist, blockade by an antagonist, as well as inhibition of ...
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