protein unfolding
Optimizing the calculation of energy landscape parameters from single-molecule protein unfolding experiments
... of protein unfolding events observed in each approach-retract cycle varies between one and five, due to the nature of the interactions between the polyprotein, the AFM tip, and the substrate, and there is ...
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Fully Atomistic Simulations of Protein Unfolding in Low Speed Atomic Force Microscope and Force Clamp Experiments with the Help of Boxed Molecular Dynamics
... of protein domains called ...some protein folds are more mechanically robust than others 2 via investigation of the mechanical unfolding pathways, which in principle should be possible by comparison ...
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To boil an egg: substrate binding affects critical stability in thermal unfolding of proteins
... widely used in screens of compound libraries, does not involve labelling and relies on the observation that the thermal stability of proteins is usually enhanced in the presence of interaction partners. In such ...
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Proteomics of hyposaline stress in blue mussel congeners (genus Mytilus): implications for biogeographic range limits in response to climate change
... indicating protein unfolding, during stress ...of protein abundances suggest that ...in protein chaperoning, vesicle transport, cytoskeletal adjustments by actin-regulatory proteins, energy ...
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Rapid and Robust Polyprotein Production Facilitates Single-Molecule Mechanical Characterization of β-Barrel Assembly Machinery Polypeptide Transport Associated Domains.
... proach, protein molecules are immobilized on a surface, and the AFM probe is repeatedly brought into contact with the ...tethered protein is ex- tended via retraction of the tip at a constant velocity 3 or ...
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Folding and Unfolding Simulations of a Three Stranded Beta Sheet Protein
... study protein folding processes [5]. However, simulation of protein-folding processes with an atomistic model is a very difficult ...including unfolding simulations start- ing from the folded state ...
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Unfolding dynamics of proteins under applied force
... Mechanical protein-unfolding experiments have been most successfully applied to polymeric proteins, ...polymeric protein to be mechanically unfolded was the giant muscle protein titin ...
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Forstater_unc_0153D_14136.pdf
... to protein unfolding[240], protein fibrillation, protein aggregation[246], and the modifications of enzy- matic ...links protein aggregation to the pathology and progression of most ...
252
Efficient unfolding pattern recognition in single molecule force spectroscopy data
... different protein unfolding pathways, and draw statistical conclusions on the unfolding events ’ locations (amino acids), their occurrences, and their co-occurrences with other events, one must be ...
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Le Brun, Virginie (2009): Physical Properties of Protein Formulations. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... induces protein unfolding that promotes in many cases ...the unfolding transition, was measured by microcalorimetry as a function of the NaCl ...lysozyme unfolding started at about 65 °C for ...
152
Thesis
... of protein unfolding, and has been described previously 18 ...the protein cannot be covalently labeled by a fluorogenic ...the protein exposes its hydrophobic core region, to which Sypro binds ...
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Ott, Wolfgang Bernhard (2017): Single molecule force spectroscopy with biological tools. Dissertation, LMU München: Fakultät für Physik
... Recently, experimental measurements were brought into prox- imity (Dong and Sahin, 2011; He et al., 2012; Schoeler et al., 2015) or even overlap (Rico et al., 2013) with all atom steered molecular dynamics (SMD) ...
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Translocation through the Conjugative Type IV Secretion System requires unfolding of its protein substrate
... on protein transport to the recipient ...modified protein substrate that is fully ...an unfolding-resistant one efficiently blocks the substrate transport, resulting in more than two orders of magni- ...
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unfolding Thailand: Tourism Promoted by 3D Motion Graphics
... Unfolding Thailand is a project that aims to bring a new approach in introducing the country as well as promoting the tourism. My specific scope of this project is to create a visual representation of popular ...
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High-Temperature unfolding of a trp-Cage mini-protein: a molecular dynamics simulation study
... this protein in explicit water have been carried out using what is known as the Replica Exchange ...high-temperature unfolding simulation of the Trp-cage mini-construct are ...
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Folding, Stability and Active Site Conformation of Procaspase-3
... the protein undergoes a pH-dependent conformational change. The protein below pH ...the protein dissoci that ...the protein undergoes a two-step unfolding ...in unfolding pattern ...
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THE UNFOLDING TALE OF LEPTIN
... Whilst naturally occurring antibodies or increased leptin binding proteins in obesity have not been demonstrated so far, there is however evidence for the absence of leptin in some obese[r] ...
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Microwave based reversible unfolding and refolding of alcohol oxidase protein probed by fluorescence and circular dichroism spectroscopy
... AOx belongs to the family of glucose-methanol-cho- line (GMC) oxidoreductases. The structure of AOx con- tains three major domains, namely an FAD-binding domain, a flavin attachment loop and a substrate binding domain, ...
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Surprise: Unfolding of facial expressions
... (Noordewier et al., 2016). Initial responses are primarily driven by the unexpectedness of the surprising outcome and re fl ect an interrupted and surprised state. Later responses are more likely to incorporate the ...
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PubMedCentral-PMC5703279.pdf
... These additional layers of regulation likely explain why our model of translation efficiency performs better in tissue culture cells than in tissues. However, our model still provides insights into the regulation of ...
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