Pulsed Field
Pulsed Field Gel Electrophoresis in Differentiation of Erysipelothrix Species Strains
... We report here the first analysis of Erysipelothrix spp. using pulsed-field gel electrophoresis (PFGE). Seventy strains of Erysipelothrix spp. were analyzed. SmaI, AscI, and NotI were tested for the ability ...
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Subtyping of Haemophilus influenzaeStrains by Pulsed Field Gel Electrophoresis
... PFGE. The DNA extracted from each agarose block prepared as described above was digested with the enzyme SmaI (Toyobo Co. Ltd., Osaka, Japan) (3, 4). Pulsed-field gel electrophoresis (PFGE) was performed in ...
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Subspecies typing of Vibrio parahaemolyticus by pulsed field gel electrophoresis
... a pulsed-field gel electrophoresis (PFGE) method for the molecular typing of this ...electric field apparatus set at 190 V and a pulse time of 3 to 80 ...
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Pulsed field magnetometry for rock magnetism
... likely to be caused by the small fractions of superpara- magnetic (SP) particles with the relaxation time in the narrow range of 10 −4 to 10 −3 s. Alternatively, they could be due to an irreversible displacement of DWs, ...
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Epidemiologic Genotyping of Methicillin Resistant Staphylococcus aureus by Pulsed Field Gel Electrophoresis at a University Hospital and Comparison with Antibiotyping and Protein A and Coagulase Gene Polymorphisms
... and pulsed-field gel ...a field strength of 6 V/cm for 24 h at 14°C, with two blocks of pulses: a first block of 12 h with 5- to 15-s pulses and a second block of another 12 h from 5- to 40-s ...
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Molecular analysis of Salmonella enteritidis by pulsed field gel electrophoresis and ribotyping
... A total of 61 isolates of Salmonella enteritidis were analyzed by the techniques of pulsed-field gel electro- phoresis (PFGE) and ribotyping. Twenty-three of the isolates were from Zurich, Switzerland, and ...
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Pulsed field gel electrophoresis as a replacement for bacteriophage typing of Staphylococcus aureus
... Outbreaks of infections caused by Staphylococcus aureus continue to be a problem for health care facilities (16). Iden- tifying strains among the outbreak isolates is a major step in determining the source of the ...
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Rapid Pulsed Field Gel Electrophoresis Protocol for Subtyping of Campylobacter jejuni
... Campylobacter jejuni is the most common bacterial cause of diarrheal illness in the United States (1). It is estimated that Campylobacter infections affect over 2.5 million persons, with 80% of infections attributable to ...
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Evaluation of Pulsed Field Gel Electrophoresis Profiles for Identification of Salmonella Serotypes
... Traditionally, phenotypic methods such as serotyping have been used for identification of Salmonella isolates in outbreak investigations. However, phenotypic methods have limited util- ity for epidemiologic analysis of ...
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In silico Pulsed Field Gel Electrophoresis and Molecular characterization of Escherichia coli
... Sixty-five isolates were analyzed for virulence, antibiotic resistance and adherence genes by in silico tools. Thirteen virulence genes were selected to classify Escherichia coli isolates. The most prevalent virulence ...
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Karyotype analysis of interspecific fusants of basidiomycetes by pulsed-field gel electrophoresis
... some authors 6-9 performed intraspecific protoplast fusion and others 8'1~ interspecific protoplast fusion of ed- ible mushrooms.. the method using a chemical fusogen has[r] ...
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emc13-proof.pdf
... initio pulsed field calculation followed by ...current, field, and antenna signal can be associated with a maximum likelihood of device destruction if detected in the ...
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Electric field Analysis of Water Electrodes for Noninvasive Pulsed Electric field Applications
... electric field distribution inside the PEF treatment chamber is supposed to have a greater impact on bacterial ...electric field distribution inside the PEF chamber depends on the configuration of the ...
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Order by quenched disorder in the model triangular antiferromagnet RbFe(MoO4)2
... We observe a disappearance of the 1 = 3 magnetization plateau and a striking change of the magnetic configuration under a moderate doping of the model triangular antiferromagnet RbFe ð MoO 4 Þ 2 . The reason is an ...
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Pulsed Electric Field Processing in Food Technology
... electrical field for a few microseconds induces local structural changes and a rapid breakdown of the cell ...intensity pulsed electric fields (HIPEF) have been studied in the last ...genetics pulsed ...
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Interaction of living cells with electric pulses: Parameters and possible applications
... electric field, following electroporation, in order to increase the diffusion rate of DNA through electropores via electrophoretic mobility (Klenchin et ...electric field intensity ...increasing ...
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The Effects of Extremely Low Frequency Pulsed Electromagnetic Field on Collagen Synthesis of Rat Skin: a Biochemical and Histoligical Approach
... electrical field components generated by the coil [17], the level of magnetic induction, the cell density, and the cell type and the assay system investigated, and finally, the effects of EMF are limited to narrow ...
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Pulsed Electric Field System Development for Algae Biofuel Extraction
... Modifications may need to be added if it is determined lysis will be achieved by applying multiple pulses to the algae in a time span shorter than 1s. One suggestion to achieve multiple pulses is to introduce additional ...
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Circuits and systems for CW and pulsed high field electron spin resonance
... The challenges of measuring the phase noise spectrum of oscillators at 7GHz and sources at 94GHz have been addressed and a low phase noise source, which provides both the main spectromet[r] ...
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Inactivation of Escherichia coli phage by pulsed electric field treatment
... electric field intensity and pulse number on the inactivation curve was showed in figure 1a and ...electric field intensity 6kV and pulse width 5µs, almost 2-log reduction was observed while increasing the ...
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