Quantitative Polymerase Chain Reaction Analysis (miRNA)
Quantitative polymerase chain reaction analysis of herpes simplex virus DNA in ganglia of mice infected with replication-incompetent mutants.
... Ganglia from mice infected with thymidine kinase-negative mutants, which replicate at the site of inoculation and establish latency but do not replicate acutely in ganglia or reactivate [r] ...
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Measurement of bioprocess containment by quantitative polymerase chain reaction
... Chapter 4. Adaptation o f the QPCR method for air sampling 4.3 Stability in solution of microbially derived plasmid pQRTOl Generally, DNA solutions are thought to be stable if stored free from microbial contamination at ...
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Diagnosis of tubercular uveitis by quantitative polymerase chain reaction
... The PCR has emerged as a powerful tool for rapid detection of the mycobacterial genome, with a high specificity and a variable sensitivity [3, 9, 11–13]. Aqueous humor, vitreous, or subretinal fluid and even tissue ...
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Analysis of normal and osteoarthritic canine cartilage mRNA expression by quantitative polymerase chain reaction
... If variables such as the methods of mRNA extraction, RNA quality assessment, reverse transcription, assay design, meas- urement of genomic contamination, standard curve data gen- eration, reference gene selection, and ...
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Analysis of normal and osteoarthritic canine cartilage mRNA expression by quantitative polymerase chain reaction
... If variables such as the methods of mRNA extraction, RNA quality assessment, reverse transcription, assay design, meas- urement of genomic contamination, standard curve data gen- eration, reference gene selection, and ...
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Analysis of Specific Bacteria from Environmental Samples using a Quantitative Polymerase Chain Reaction
... 2 Laragen Inc., 10755 Venice Ave., Los Angeles, CA 90034, USA Abstract This article describes the use of quantitative PCR for measuring bacterial abundance in environmental samples. The two approaches discussed ...
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New Developments in Quantitative Real-time Polymerase Chain Reaction Technology
... The first step in RT-qPCR based gene expression analysis is the faithful conversion of RNA to cDNA by reverse transcription. Therefore, the first step would be the development of an RT enzyme which is able to ...
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Gene expression of the renin angiotensin system in human tissues Quantitative analysis by the polymerase chain reaction
... the polymerase chain reaction (PCR) after reverse transcription to detect renin- angiotensinogen-, and angiotensin-converting enzyme-mRNA in small quantities of human ...
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Detection of human herpesvirus 8 by quantitative polymerase chain reaction: development and standardisation of methods
... V: Quantitative analysis of human herpesvirus 8 viral load using a real-time PCR ...herpesvirus-8 polymerase chain reaction in the evaluation of Kaposi sarcoma and other vascular tumors ...
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The efficacy of quantitative fluorescent-polymerase chain reaction (QF-PCR) in the diagnosis of prenatal aneuploidy
... Although the specificity and sensitivity values were not very high in the present study, in the future, following technological development of these tests, their efficacy may be expected to increase, shortening the ...
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Development of multiplexed real-time quantitative polymerase chain reaction assay for detecting human adenoviruses.
... one reaction or 2 reactions have been published also (Chmielewicz B et al 2005; Gu Z et al 2003; Ebner K et al 2006; Ebner K et al ...length analysis have been published (Miura-Ochiai R et al 2007; Ebner K ...
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Selection of suitable reference genes for quantitative real time polymerase chain reaction in human meningiomas and arachnoidea
... Expression stability of eight reference genes plus GAPDH and ACTB in meningiomas, arachnoidea, dura and normal brain To validate the expression stability of CASC3, EIF2B1, IPO8, MRPL19, PGK1, POP4, PPIA, RPL37A plus ...
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Reference genes for quantitative reverse transcription polymerase chain reaction expression studies in wild and cultivated peanut
... expression analysis across various organs and type of stresses in different Arachis species, contributing particularly for the under- standing of stress tolerance/resistance mechanisms in ...
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Development of a fluorescent quantitative real time polymerase chain reaction assay for the detection of Goose parvovirus in vivo
... this analysis revealed that the blood, heart, liver, spleen, kidney, Bursa of Fabricius (BF), thy- mus, and Harder's glands were positive at 4-h postinocu- lation (PI), with about 10 ...
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Quantitative Real-time Polymerase Chain Reaction for Tracking Microbial Gene Expression in Complex Environmental Matrices
... Conclusions It is beyond doubt that there has been resurgence in the application of nucleic acid targeting tools in every aspect of biology, owing to reduced technology costs, constant development of effective nucleic ...
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Antiadenoviral effects of <em>N</em>-chlorotaurine in vitro confirmed by quantitative polymerase chain reaction methods
... N-Chlorotaurine (Cl – HN – CH 2 – CH 2 – SO 3 H, NCT) is the N-chloro derivative of the amino acid taurine. NCT is the main representative of long-lived oxidants produced by stimulated granulocytes and monocytes during ...
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Real-time quantitative PCR (polymerase chain reaction)- page 47
... Fig.28C: Relative fold change of CRBN mRNA level in peripheral blood (PB) mononuclear cells of female patient with with del(5q) and trisomy 8 in different clones during len[r] ...
Establishment of Micro Droplet Digital Polymerase Chain Reaction and Real-Time Fluorescence Quantitative Polymerase Chain Reaction Technologies for Detecting Zika Virus
... established a method for ZIKA nucleic acid detection using ddPCR and qPCR. Our results 206[r] ...
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THE advent of the polymerase chain reaction in the
... Ruane and Austin first identified 21 snakes from museum collections (primarily the California Academy of Sciences and the Museum of Comparative Zoology at Harvard University), representi[r] ...
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