quantitative real-time-PCR
Quantitative real-time PCR (qPCR) for Eimeria tenella replication – implications for experimental refinement and animal welfare
... non-subjective, quantitative, tools to measure parasite replication in the host. The time- consuming, and often time-sensitive, nature of existing approaches precludes their use in large-scale ...
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A triplex quantitative real time PCR assay for differential detection of human adenovirus serotypes 2, 3 and 7
... traditional PCR method to detect serotypes of adenovirus [25, 26] needs gel electrophor- esis and sequencing, which significantly increases the risk of cross-contamination and is also time consuming and ...
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Rapid Screening of Recombinant Plasmids by Direct Colony Quantitative Real Time PCR
... CRISPR/Cas9 system is a novel powerful tool for mammalian genome editing by simply specifying a 20-nt targeting sequence within its guide RNA [1]. Currently we use this system extensively in our laboratory to establish ...
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Development of a Quantitative Real Time PCR Assay for Detection of Mycoplasma genitalium
... the PCR is ex- pected to miss exp( ⫺ 2) ⫻ 100% ⫽ ...Other real-time PCR methods have been devel- oped for the detection of ...LightCycler PCR assay by Dupin et ...LightCycler ...
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Multiplex, Quantitative, Real Time PCR Assay for Cytomegalovirus and Human DNA
... MQR PCR assay is sensitive for the detection of CMV, with the ability to amplify 1 to 10 CMV copies per reaction ...the quantitative assay by ensuring that the quantitative plasmid standards ...
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Validation and implementation of hepatitis b viral load detection with quantitative real time PCR
... and time PCR for Hepatitis B viral load ...digital PCR for the preparation of in-house standards also makes the time HBV assay was validated for its linearity, sensitivity, precision, ...
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Identification of reliable reference genes for quantitative real-time PCR normalization in pitaya
... To the best of our knowledge, this study is the first report on systematically evaluating the expression sta- bility of different potential reference genes for qRT-PCR in the Cactaceae family. Thirty-nine typical ...
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A Quick and Efficient Method to Quantify Baculo Virus by Quantitative Real Time PCR
... and time consuming. The real- time PCR assay has the advantage of speed and ...novel quantitative PCR (qPCR) method, using Baculovirus gene gp64 (Coat protein) specific primers ...
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Comparison between Quantitative Nucleic Acid Sequence Based Amplification, Real Time Reverse Transcriptase PCR, and Real Time PCR for Quantification of Leishmania Parasites
... potential quantitative characteristics in comparison with conventional diagnostic methods but are often still not routinely ...by quantitative nucleic acid sequence-based amplification (QT-NASBA), ...
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Original Article Spatiotemporal expression of Wnt5a during the development of the striated muscle complex in rats with anorectal malformations
... and quantitative real-time PCR (qRT-PCR) between normal rat embryos and embryos with ...same time-dependent changes of Wnt5a protein were detected in ARMs ...the time of ...
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circGFRA1 and GFRA1 act as ceRNAs in triple negative breast cancer by regulating miR-34a
... In this study, we analyzed the expression profiles of cir- cRNAs in TNBC cell lines through microarrays. Expression levels of a significantly upregulated circRNA, circGFRA1, was detected by quantitative ...
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Comparison of Droplet Digital PCR to Real Time PCR for Quantitative Detection of Cytomegalovirus
... Quantitative real-time PCR (QRT-PCR) has been widely implemented for clinical viral load testing, but a lack of standardization and relatively poor precision have hindered its ...
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Angiogenesis and vasculogenic mimicry are inhibited by 8-Br-cAMP through activation of the cAMP/PKA pathway in colorectal cancer
... 8-Br-cAMP; quantitative real-time-PCR (qRT-PCR) demonstrated that 8-Br-cAMP regulated the expression of vascular endothelial (VE)-cadherin, matrix metalloproteinase 2 (MMP2), ephrin ...
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Dysregulation of miR-15a and miR-214 in human pancreatic cancer
... by quantitative real time-PCR (qRT-PCR) and found that miR-15a and miR-214 were dysregulated in the tumor ...first time that miR-214 has been identified as aberrantly expressed ...
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Comparison of a New Quantitative ompA Based Real Time PCR TaqMan Assay for Detection of Chlamydia pneumoniae DNA in Respiratory Specimens with Four Conventional PCR Assays
... involving real-time PCR protocols (16, 23) have shown the overall superiority of this technique over con- ventional ...RUO PCR from Abbott Laboratories should ensure consistent ...with ...
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Real Time Quantitative PCR for Detection of Helicobacter pylori
... The classical protocol for isolating nucleic acid from rela- tively large amounts of tissue (200 mg to 1 g) is the proteinase K-phenol method. For isolating nucleic acids from small amounts of tissue, there are no ...
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Quantitative Detection of Borrelia burgdorferi by Real Time PCR
... The data obtained by our new method provide evidence of a positive correlation between clinical symptoms, measured as arthritis and spirochete burden in the mouse model. First, the numbers of spirochetes were ...
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Real Time PCR for Quantitative Detection ofToxoplasma gondii
... Several PCR-based techniques have been developed as alternative diagnostic measurements for ...combined PCR amplification with Southern blotting to detect a specific B1 gene ...heminested PCR method ...
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Establishment and Application of Digital RT PCR Assay for Detection of Avian Influenza Virus H9 Subtype
... A digital RT-PCR method for rapid detection of H9 subtype influenza was es- tablished by comparing the two methods of digital RT-PCR and real-time quantitative RT-PCR. The ...
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Simultaneous Detection of Influenza Viruses A and B Using Real Time Quantitative PCR
... 25-l PCR was performed using 5 l of cDNA, 12.5 l of TaqMan universal PCR master mix containing ROX as a passive reference (PE Applied Biosystems), 900 nM each influenza virus A primer, 300 nM each ...
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