Quantitative Real Time Polymerase Chain Reaction
Quantitative Real-time Polymerase Chain Reaction for Tracking Microbial Gene Expression in Complex Environmental Matrices
... Viability status of the target microorganism is extremely critical to accurately determine the biosafety status of a food sample (Keer and Birch, 2003). The conventional cultivation protocol even though highly ...
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Development of a fluorescent quantitative real time polymerase chain reaction assay for the detection of Goose parvovirus in vivo
... The FQ-PCR amplification curves and the corresponding FQ-PCR standard curve (Figure 1) were generated by employing the successively diluted known copy numbers of pVP3 for real-time PCR reaction under ...
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Selection of suitable reference genes for quantitative real time polymerase chain reaction in human meningiomas and arachnoidea
... results from the four algorithms an integration tool for analyzing reference genes expression was used http:// www.leonxie.com/referencegene.php. First according to the reference genes ranking by every algorithm from the ...
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New Developments in Quantitative Real-time Polymerase Chain Reaction Technology
... DNA polymerase, any chemical moiety, which is able to alter the fidelity (negatively) of these enzymes, has a direct bearing on the end results (Suslov and Steindler, ...RT-qPCR reaction which are known to ...
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Seven-microRNA panel for lung adenocarcinoma early diagnosis in patients presenting with ground-glass nodules
... The subset of miRNAs found to be differentially expressed in tumor versus non-tumor analysis by NGS was assayed by quantitative real-time polymerase chain reaction (qRT- PCR) in ...
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Clinicopathological and prognostic significance of long noncoding RNA MALAT1 in human cancers: a review and meta-analysis
... qRT-PCR: quantitative real-time polymerase chain reaction; ISH: in situ hybridization; HWE: hardy–weinberg equilibrium; HR: hazard ratio; OS: overall survival; DFS: disease-free ...
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PubMedCentral-PMC4931989.pdf
... performed quantitative real-time polymerase chain reaction (PCR) with these upregulated miRNAs from the miRNA microarray, and 8 miRNAs showed significantly increased expression ...
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Long noncoding RNA CASC2 predicts the prognosis of glioma patients and functions as a suppressor for gliomas by suppressing Wnt/β-catenin signaling pathway
... Notes: (A–C) β-catenin, cyclin-D1, and c-Myc were upregulated in glioma tissues compared with adjacent normal brain tissues, as found by qrT-Pcr analysis. (D) Overexpression of casc2 suppressed the protein expression of ...
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Regeneration and reprogramming compared
... by quantitative real time polymerase chain reaction (qPCR) of regenerating and non-regenerating tissue compared to an embryonic, pluripotent reference cell type, analysis of the ...
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Asymmetric effects of native and exotic invasive shrubs on ecology of the West Nile virus vector Culex pipiens (Diptera: Culicidae)
... development time, and adult body ...using quantitative real-time polymerase chain reaction to test the hypothesis that variation in emergence, development, and oviposition ...
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The prognostic values of the expression of Vimentin, TP53, and Podoplanin in patients with cervical cancer
... Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot analysis were performed to determine the messenger RNA and protein expression levels of ...
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Analyzing the gene expression profile of pediatric acute myeloid leukemia with real-time PCR arrays
... by quantitative real-time polymerase chain reaction in 94 consecutive childhood acute lymphoblastic leukaemia (ALL) samples and its association with clinical/biological features ...
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Original Article NF-κB and STAT6 signaling transduction pathways in immunoglobulin E production of U266 cell
... Using quantitative real-time polymerase chain reaction, Western blotting, and enzyme-linked immunosorbent assays, we found that the U266 cell stimulated with the cytokine ...
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Altered Expression of Circulating miR-377 and miR-98 in Relapsing-remitting Multiple Sclerosis
... a quantitative real-time polymerase chain reaction method to explore the expression of circulating miR-377 and miR-98 in peripheral blood mononuclear cells from 60 RRMS patients, ...
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<p>Effects of dynein light chain Tctex-type 3 on the biological behavior of ovarian cancer</p>
... by quantitative real-time polymerase chain reaction and Western blotting respectively, and the proliferation, apoptosis, migration and invasion of SKOV3 cells after DYNLT3 ...
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Quantitative detection of Streptococcus mutans from saliva using FTATM elute cards and real time polymerase chain reaction
... Each reaction contained 5 µL DNA template from disc extracts, 10 µL of LightCycler 480 SYBR Green I Mas- ter (Roche diagnostics Gmbh, Germany), 1 µL of 10 µM forward and reverse primer ...the reaction mix ...
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Original Article Novel real-time polymerase chain reaction assay for quantitative detection of Chlamydia trachomatis in vaginal and cervical secretions
... developed real-time PCR method is more sensitive and specific, espe- cially in terms of quantitative detection of ...samples, real-time PCR analysis revealed a positive rate of ...novel ...
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Lack of a skeletal muscle phenotype in adult human bone marrow stromal cells following xenogeneic-free expansion
... digital polymerase chain reaction (ddPCR) has been shown to be advantageous compared to real-time quantitative PCR due to its in- creased accuracy, reproducibility, and ...
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Sulfur mustard induces expression of metallothionein-1A in human airway epithelial cells
... Quantitative real-time reverse transcriptase polymerase chain reaction was then performed in a Rotor-Gene RG 3000 (Corbett Research, Mortlake, ...instructions. ...
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Development of multiplexed real-time quantitative polymerase chain reaction assay for detecting human adenoviruses.
... Our initial studies in developing the assay illustrated the complexities in multiplexing real time PCR assays. Inhibition was common when too many primer probe sets were put together. Only through a series ...
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