Quantitative real-time polymerase chain reaction
Evaluation of reference genes for gene expression studies in senna (Cassia angustifolia Vahl.) using quantitative real-time polymerase chain reaction.
... Quantitative real-time polymerase chain reaction (RT-qPCR) is one of the most common methods used for quantification of gene expression due to its high sensitivity, specificity, ...
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Research Article. Keywords: 3D culture, hepatocellular carcinoma cell line, housekeeping gene, quantitative real time polymerase chain reaction
... different time phases is signi cantly important for disease ...is quantitative real time polymerase chain reaction (RT-qPCR) due to its simplicity, speci city, and ...
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Reverse transcription quantitative real time polymerase chain reaction reference genes in the spared nerve injury model of neuropathic pain: validation and literature search
... semi-quantitative polymerase chain reaction, RT-qPCR reverse transcription quantitative real-time polymerase chain reaction, SC spinal cord, DRG ...
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Identification of human intestinal parasites affecting an asymptomatic peri urban Argentinian population using multi parallel quantitative real time polymerase chain reaction
... Methods: The study was carried out in Orán, Argentina. A total of 99 stool samples from a local surveillance campaign were analyzed by concentration microscopy and McMaster egg counting technique compared to the analysis ...
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New Developments in Quantitative Real-time Polymerase Chain Reaction Technology
... The net effect of such interferences, at least in RT-qPCR, is the invariable quenching of the fluorescent signal, leading to sub-optimal or irreproducible gene expression data. Consequently, solutions are constantly ...
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Selection of suitable reference genes for quantitative real time polymerase chain reaction in human meningiomas and arachnoidea
... www.leonxie.com/referencegene.php. First according to the reference genes ranking by every algorithm from the most stable gene to the least stable gene, a series of con- tinuous integers starting from 1 as weight to each ...
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Development of a fluorescent quantitative real time polymerase chain reaction assay for the detection of Goose parvovirus in vivo
... GPV detection by real-time PCR has only been reported by Bi [20]; in that study, the method was not optimized and a FQ-PCR standard curve was not generated. In this study, we reported the optimization of a ...
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Quantitative Real-time Polymerase Chain Reaction for Tracking Microbial Gene Expression in Complex Environmental Matrices
... To overcome some of the well known technical limitations of end-point PCR, detection platforms like RT-qPCR have proven to be an ideal solution. Using this highly versatile technology, it is now possible to ...
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Ex vivo screening for immunodominant viral epitopes by quantitative real time polymerase chain reaction (qRT PCR)
... carcinoma [3]. Simian virus 40 (SV40) has been associ- ated with mesothelioma [4,5] and Human Papiloma Virus (HPV) with cervical cancer [6] and it appears that T cell reactivity may control their growth. Thus, we have ...
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Absolute quantitative real-time polymerase chain reaction for the measurement of human papillomavirus E7 mRNA in cervical cytobrush specimens
... DNA isolation and detection Viral DNA was extracted from cervical cytobrush speci- mens using a commercially available kit (QIAamp DNA Mini Kit, Qiagen, Valencia, CA, USA) according to manu- facturer's buccal swab spin ...
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SYBR green based one step quantitative real time polymerase chain reaction assay for the detection of Zika virus in field caught mosquitoes
... specific real-time reverse transcription polymerase chain reaction (rRT- PCR) assay for the detection of ZIKV in field-caught ...
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Real-time quantitative PCR (polymerase chain reaction)- page 47
... B.) Fig.16B: Values of hemoglobin level (g/l) are shown as function of time during lenalidomide (LEN) treatment of female MDS patient del(5q) and trisomy 8 in different clones. The patient reached complete ...
Establishment of Micro Droplet Digital Polymerase Chain Reaction and Real-Time Fluorescence Quantitative Polymerase Chain Reaction Technologies for Detecting Zika Virus
... established a method for ZIKA nucleic acid detection using ddPCR and qPCR. Our results 206[r] ...
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Development of multiplexed real-time quantitative polymerase chain reaction assay for detecting human adenoviruses.
... Multiplexing primers and probes into one reaction or 2 reactions have been published also (Chmielewicz B et al 2005; Gu Z et al 2003; Ebner K et al 2006; Ebner K et al 2005). All these assays have been proved to ...
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Measurement of bioprocess containment by quantitative polymerase chain reaction
... It has been pointed out (Ferris, 1995) that the cyclone is not an easy device to use and that a good deal of experience is required before it can be operated effectively and reproducibly. Some important modifications ...
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Diagnosis of tubercular uveitis by quantitative polymerase chain reaction
... the real-time amplification was followed by melting curve analysis from 65°C to ...the reaction mixtures of 25 μl were 600 nM for each forward and reverse ...
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Quantitative determination, by real time reverse transcription polymerase chain reaction, of aromatase mRNA in invasive ductal carcinoma of the breast
... Real-time reverse transcription polymerase chain reaction PCR was performed using a LightCycler. The PCR reac- tion was carried out in a 20 µ l final volume containing the following: H ...
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Quantitative detection of Streptococcus mutans from saliva using FTATM elute cards and real time polymerase chain reaction
... and Real-Time Polymerase Chain Reaction to a conventional culture-based assay using oral ...able quantitative results, covering a large range of bacterial ...
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Performance evaluation of optimal real-time polymerase chain reaction achieved with reduced voltage
... the time to reach the target temperature, so the pattern was reduced to a smaller size (hereafter, small-sized pattern) that would only heat the reservoir ...short time was needed to reach the target ...
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Original Article Novel real-time polymerase chain reaction assay for quantitative detection of Chlamydia trachomatis in vaginal and cervical secretions
... Quantitative detection in cell culture superna- tant and the mouse model Cell culture supernatants and mouse model specimens containing Chlamydia EBs were used to verify the feasibility of Chlamydia EB detection ...
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