random mutagenesis
Random Mutagenesis Defines a Domain of Theiler's Virus Leader Protein That Is Essential for Antagonism of Nucleocytoplasmic Trafficking and Cytokine Gene Expression
... Influence of selected L mutations in the context of viral infections. To test whether the Theilo domain mutations also affected other functions of the L protein such as the inhibition of cytokine and chemokine gene ...
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PTO-QuickStep : a fast and efficient method for cloning random mutagenesis libraries
... condition was used. After the epPCR and iodine cleavage, we proceeded to megaprimer PCR as per usual. Again, for a more objective comparison, 25 cycles of megaprimer PCR were used. Concurrently, we prepared another ...
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Development of simple random mutagenesis protocol for the protein expression system in Pichia pastoris
... for random mutagen- esis, because this organism has the capability to per- form many eukaryotic post-translational ...convenient random mutagenesis method that does not require laborious steps such ...
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Optimization of lipid production by the oleaginous yeast Lipomyces starkeyi by random mutagenesis coupled to cerulenin screening
... In this work we performed assays for the genetic improvement of the oleaginous yeast Lipomyces starkeyi DSM 70296 focusing on its utilization for lipid biosynthesis from renewable sources. The genetic optimization was ...
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Random mutagenesis of NS1 protein of influenza a H1N1 and docking of RNA aptamers to wild type and mutant NS1 proteins
... There were several parts of research activity in this project including mutagenesis, cloning, multiple sequence alignment, protein modeling and molecular docking. The NS1A gene from clone 104 of pET-32c(+) vector ...
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Development of an inducible transposon system for efficient random mutagenesis in Clostridium acetobutylicum
... By way of contrast, scant attention has been paid to for- ward genetic approaches reliant on random mutagens typified by transposon elements. Two conjugative transposons Tn916 and Tn1514 have been studied in C. ...
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Random mutagenesis of the thymidine kinase gene of varicella-zoster virus.
... Nucleotide and predicted amino acid sequences of the Marek's disease virus and turkey herpesvirus thymidine kinase genes; comparison with thymidine kinase genes of other herpesviruses.. [r] ...
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Two classes of human papillomavirus type 16 E1 mutants suggest pleiotropic conformational constraints affecting E1 multimerization, E2 interaction, and interaction with cellular proteins.
... Random mutagenesis of HPV16 E1 was performed as previously described (51, 52; see also Fig. 2). Briefly, mutagenic PCR using oligonucleotide primers GCGTAGTACAGCAGCAGC (nucleotides [nt] 1729 to 1816) and ...
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Genetic manipulation of some bacterial strains for improvement of PUFA production
... MMS mutagenesis are compared with published literature , it is noticeable that similar results displaying superior growth and lipid accumulation in mutants generated by random mutagenesis of ...
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A novel cold-adapted esterase from Enterobacter cloacae: Characterization and improvement of its activity and thermostability via the site of Tyr193Cys
... Background: In industries lipolytic reactions occur in insensitive conditions such as high temperature thus novel stout esterases with unique properties are attracts to the industrial application. Protein engineering is ...
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Enhancing butanol tolerance of Escherichia coli reveals hydrophobic interaction of multi-tasking chaperone SecB
... strain. Random mutagenesis of SecB was implemented and homologously overexpressed in ...Saturation mutagenesis on T10 site revealed that hydrophobic residues were required for high butanol tolerance ...
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zjv9113.pdf
... Shuffling and random mutagenesis of isolated clones for additional screening. The four highest-affinity scFv genes isolated from the off-rate analysis described above, along with the 80R scFv gene, were ...
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Hypersusceptibility to Substrate Analogs Conferred by Mutations in Human Immunodeficiency Virus Type 1 Reverse Transcriptase
... to random mutagenesis altered viral susceptibility to one or more nucleoside analogs, and several mutations con- ferred altered sensitivity to the pyrophosphate analog ...
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Increased Antibody Affinity Confers Broad In Vitro Protection against Escape Mutants of Severe Acute Respiratory Syndrome Coronavirus
... Shuffling and random mutagenesis of isolated clones for additional screening. The four highest-affinity scFv genes isolated from the off-rate analysis described above, along with the 80R scFv gene, were ...
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Random Transposon-Mediated Mutagenesis of the Essential Large Tegument Protein pUL36 of Pseudorabies Virus
... Homologs of the pseudorabies virus (PrV) essential large tegument protein pUL36 are conserved throughout the Herpesviridae. pUL36 functions during transport of the nucleocapsid to and docking at the nuclear pore as well ...
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Rapid sitrapid site-directed domain scanning mutagenesis of enteropathogenic Escherichia coli espD
... scanning mutagenesis by inserting a signature tag in-frame of a gene of interest is a powerful way to study the domain functions of a gene of ...interest. Random mutagenesis methods based on ...
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The phage T4 DNA ligase in vivo improves the survival-coupled bacterial mutagenesis
... microbial mutagenesis was explored by mutating and screening PHB producing ...microbial mutagenesis, large survival pool was ...traditional random mutagenesis became more powerful in combine ...
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Directed evolution and secretory expression of a pyrethroid-hydrolyzing esterase with enhanced catalytic activity and thermostability
... The mutant library was screened by a two-step screen- ing strategy. Initially, the transformants were repli- cated on LB agar plates supplemented with 0.1% (v/v) tributyrin, 100 μg/mL ampicillin and 0.1 mM IPTG ...
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Directed Evolution of Terpene Synthases for Non Natural Substrates
... using random mutagenesis, with error prone polymerase chain reactions, and using site-specific saturation mutagenesis, in which an NNK library is designed with a specific active site amino acid ...
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The Applications of Promoter-Gene Engineered Bio-Sensors
... Error-prone PCR and site-directed mutagenesis which is applied for construction of bacteria’s “mutant library”. It is expected to establish flexible HTS platform (Bio-sensor array) to large-scale screen ...
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