real-time quantitative polymerase chain reaction
Rapid Prenatal Diagnosis of Trisomy 21 by Real time Quantitative Polymerase Chain Reaction with Amplification of Small Tandem Repeats and S100B in Chromosome 21
... using real-time quantitative polymerase chain reaction (PCR) of fetal DNA from amniotic ...fluid. Real-time quantitative PCR was performed with DNA templates ...
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Development of multiplexed real-time quantitative polymerase chain reaction assay for detecting human adenoviruses.
... Multiplexing primers and probes into one reaction or 2 reactions have been published also (Chmielewicz B et al 2005; Gu Z et al 2003; Ebner K et al 2006; Ebner K et al 2005). All these assays have been proved to ...
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Real-time quantitative PCR (polymerase chain reaction)- page 47
... B.) Fig.16B: Values of hemoglobin level (g/l) are shown as function of time during lenalidomide (LEN) treatment of female MDS patient del(5q) and trisomy 8 in different clones. The patient reached complete ...
New Developments in Quantitative Real-time Polymerase Chain Reaction Technology
... The net effect of such interferences, at least in RT-qPCR, is the invariable quenching of the fluorescent signal, leading to sub-optimal or irreproducible gene expression data. Consequently, solutions are constantly ...
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Establishment of Micro Droplet Digital Polymerase Chain Reaction and Real-Time Fluorescence Quantitative Polymerase Chain Reaction Technologies for Detecting Zika Virus
... established a method for ZIKA nucleic acid detection using ddPCR and qPCR. Our results 206[r] ...
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Selection of suitable reference genes for quantitative real time polymerase chain reaction in human meningiomas and arachnoidea
... www.leonxie.com/referencegene.php. First according to the reference genes ranking by every algorithm from the most stable gene to the least stable gene, a series of con- tinuous integers starting from 1 as weight to each ...
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Development of a fluorescent quantitative real time polymerase chain reaction assay for the detection of Goose parvovirus in vivo
... GPV detection by real-time PCR has only been reported by Bi [20]; in that study, the method was not optimized and a FQ-PCR standard curve was not generated. In this study, we reported the optimization of a ...
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Quantitative Real-time Polymerase Chain Reaction for Tracking Microbial Gene Expression in Complex Environmental Matrices
... To overcome some of the well known technical limitations of end-point PCR, detection platforms like RT-qPCR have proven to be an ideal solution. Using this highly versatile technology, it is now possible to ...
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Ex vivo screening for immunodominant viral epitopes by quantitative real time polymerase chain reaction (qRT PCR)
... carcinoma [3]. Simian virus 40 (SV40) has been associ- ated with mesothelioma [4,5] and Human Papiloma Virus (HPV) with cervical cancer [6] and it appears that T cell reactivity may control their growth. Thus, we have ...
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Measurement of bioprocess containment by quantitative polymerase chain reaction
... It has been pointed out (Ferris, 1995) that the cyclone is not an easy device to use and that a good deal of experience is required before it can be operated effectively and reproducibly. Some important modifications ...
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Diagnosis of tubercular uveitis by quantitative polymerase chain reaction
... the real-time amplification was followed by melting curve analysis from 65°C to ...the reaction mixtures of 25 μl were 600 nM for each forward and reverse ...
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Quantitative determination, by real time reverse transcription polymerase chain reaction, of aromatase mRNA in invasive ductal carcinoma of the breast
... Real-time reverse transcription polymerase chain reaction PCR was performed using a LightCycler. The PCR reac- tion was carried out in a 20 µ l final volume containing the following: H ...
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Absolute quantitative real-time polymerase chain reaction for the measurement of human papillomavirus E7 mRNA in cervical cytobrush specimens
... DNA isolation and detection Viral DNA was extracted from cervical cytobrush speci- mens using a commercially available kit (QIAamp DNA Mini Kit, Qiagen, Valencia, CA, USA) according to manu- facturer's buccal swab spin ...
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Quantitative detection of Streptococcus mutans from saliva using FTATM elute cards and real time polymerase chain reaction
... and Real-Time Polymerase Chain Reaction to a conventional culture-based assay using oral ...able quantitative results, covering a large range of bacterial ...
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Performance evaluation of optimal real-time polymerase chain reaction achieved with reduced voltage
... the time to reach the target temperature, so the pattern was reduced to a smaller size (hereafter, small-sized pattern) that would only heat the reservoir ...short time was needed to reach the target ...
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Original Article Novel real-time polymerase chain reaction assay for quantitative detection of Chlamydia trachomatis in vaginal and cervical secretions
... Quantitative detection in cell culture superna- tant and the mouse model Cell culture supernatants and mouse model specimens containing Chlamydia EBs were used to verify the feasibility of Chlamydia EB detection ...
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Establishment of real time quantitative reverse transcription polymerase chain reaction assay for transcriptional analysis of duck enteritis virus UL55 gene
... [30]. Real time quantitative PCR became an attractive method to study gene expression because of its low inter-assay and intra- assay variablilty and its equivalent or greater analytical sensitivity ...
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Research Article. Keywords: 3D culture, hepatocellular carcinoma cell line, housekeeping gene, quantitative real time polymerase chain reaction
... different time intervals in 3D scaffold of alginate-carboxymethylcellulose polymer- based ...different time intervals of 3D culture, as evident in ...reliable quantitative real time ...
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Evaluation of reference genes for gene expression studies in senna (Cassia angustifolia Vahl.) using quantitative real-time polymerase chain reaction.
... advantage over traditional PCR detection. RT-qPCR is the most accurate and sensitive method to study gene expression with low concentrations of mRNA in any tissues (Bustin and Nolan, 2004; Kumar et al., 2011). Although ...
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Development of real time polymerase chain reaction for detection of Salmonella typhimurium and Salmonella enteritidis in fish
... analysis time and reduces the power of PCR in terms of its rapid analysis when the result is needed immediately, especially during an ...the real time PCR performances, as well as c om par ing t he ...
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