Real-Time Quantitative RT-PCR
Classification and risk stratification of invasive breast carcinomas using a real time quantitative RT PCR assay
... the quantitative data across the methods showed a high correlation. Real-time qRT-PCR is attractive for clinical use because it is fast, reproducible, tissue-sparing, quantitative, ...
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Accurate normalization of real time quantitative RT PCR data by geometric averaging of multiple internal control genes
... Several variables need to be controlled for in gene-expres- sion analysis, such as the amount of starting material, enzy- matic efficiencies, and differences between tissues or cells in overall transcriptional activity. ...
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Broadly Reactive and Highly Sensitive Assay for Norwalk Like Viruses Based on Real Time Quantitative Reverse Transcription PCR
... transcription- PCR (RT-PCR) that is highly sensitive to a broad range of ...a real-time quantitative RT-PCR ...available RT-PCR ...
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FOXD3 is a novel tumor suppressor that affects growth, invasion, metastasis and angiogenesis of neuroblastoma
... 2E). Real- time quantitative RT-PCR analyses showed the down- regulated transcript levels of FOXD3 and NDRG1 and up-regulated transcript levels of VEGF and MMP-9 in NB cells transfected ...
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Relationship between intratumoral expression of genes coding for xenobiotic metabolizing enzymes and benefit from adjuvant tamoxifen in estrogen receptor alpha positive postmenopausal breast carcinoma
... ERα real-time quantitative RT-PCR assay [23]; complete histological and biological information available from the primary tumors; no radiotherapy or chemotherapy before surgery; and ...
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Digital karyotyping reveals probable target genes at 7q21.3 locus in hepatocellular carcinoma
... Background: Hepatocellular carcinoma (HCC) is a worldwide malignant liver tumor with high incidence in China. Subchromosomal amplifications and deletions accounted for major genomic alterations occurred in HCC. Digital ...
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Establishment and Application of Digital RT PCR Assay for Detection of Avian Influenza Virus H9 Subtype
... digital RT-PCR method for rapid detection of H9 subtype influenza was es- tablished by comparing the two methods of digital RT-PCR and real-time quantitative ...
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Original Article An allele specific locked nucleic acid real time quantitative PCR for detection of HBV rtA181V and rtN236T mutations
... LNA PCR to quan- tify rtA181V and rtN236T mutations associat- ed with ADV ...Moreover, RT-AS-LNA-qPCR assay could detect rtA181 and rtN236 mutations as well as some ADV mutations which were not identified ...
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Hepatitis E Virus (HEV) Detection and Quantification by a Real Time Reverse Transcription PCR Assay Calibrated to the World Health Organization Standard for HEV RNA
... A real-time reverse transcription-quantitative PCR (RT-qPCR) assay utilizing Pleiades probe chemistry and an RNA internal control for the simultaneous detection and quantification of HEV ...
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Highly Sensitive Methods Based on Seminested Real Time Reverse Transcription PCR for Quantitation of Human Immunodeficiency Virus Type 1 Unspliced and Multiply Spliced RNA and Proviral DNA
... seminested real-time PCR ...single-step real-time (RT-)PCR methods was suboptimal for the quantitative detection of HIV-1 RNA and DNA in PBMC from patients ...
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Superiority of Digital Reverse Transcription PCR (RT PCR) over Real Time RT PCR for Quantitation of Highly Divergent Human Rhinoviruses
... transcription-quantitative PCR (RT-qPCR) and reverse transcription-digital PCR (RT-dPCR) for quantifying HRV RNA using genotype-specific primers and probes and a consensus primer/probe ...
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Characterisation of an efficient atrazine-degrading bacterium, Arthrobacter sp. ZXY-2: an attempt to lay the foundation for potential bioaugmentation applications
... utilised real-time quantitative PCR (RT-qPCR) analysis to measure the transcriptional expression of three atrazine-degrading genes throughout the entire degradation ...
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Original Article TTLL12 expression in ovarian cancer correlates with a poor outcome
... used quantitative real-time RT-PCR and western blot to determine the expressions of TTLL12 in ovarian cancer cells and tissues and also performed immunohistochemical staining to examine ...
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Use of FTA Cards To Transport Throat Swabs and Oral Fluid Samples for Molecular Detection and Genotyping of Measles and Rubella Viruses
... transcription-quantitative real-time PCR [RT-qPCR]) and genotyping (endpoint RT-PCR) were compared for samples from 238 suspected cases; these samples were either ...
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Determination of the exact copy numbers of particular mRNAs in a single cell by quantitative real time RT PCR
... of PCR bands using conventional ...long time, it could not be used to measure mRNAs that turned over ...of PCR of Lymnaea CREB2 ...of PCR of Lymnaea CREB1 ...
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Research Progress on Reference Genes of Insect for Quantitative Real time Reverse Transcription PCR (RT qPCR)
... Quantitative real-time reverse transcription PCR (RT-qPCR) is invented by Applied Biosystems company in the United States in 1996, has become the most important method for the ...
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Paediatric acute myeloid leukaemia with the t(7;12)(q36;p13) rearrangement: a review of the biological and clinical management aspects
... by real time quantitative PCR (RT-Q-PCR) and did not observe HLXB9 expression in healthy CD34-positive bone mar- row cells, but described increased levels in the bone marrow of ...
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Original Article mRNA expression level and clinical value of hypoxia inducible factor 1 alpha in patients with myelodysplastic syndromes: a fluorescence quantitative real-time RT-PCR study
... Abstract: Objective: The clinical value and prospective function of hypoxia inducible factor 1 alpha (HIF-1α) in my- elodysplastic syndromes (MDS) remain poorly understood. Therefore, the objective of the current ...
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Concurrent Genotyping and Quantitation of Cytomegalovirus gB Genotypes in Solid Organ Transplant Recipients by Use of a Real Time PCR Assay
... a real-time genotyping and quantitative PCR (RT-GQ-PCR) assay to genotype cytomeg- alovirus (CMV) and quantify viral loads simultaneously in solid organ transplant (SOT) ...the ...
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Quantitative real-time RT-PCR of CD24 mRNA in the detection of prostate cancer
... Our aim was to determine whether QRT-PCR-based detec- tion of CD24 will yield data with sensitivity and specifi- city comparable to those in existing reports based entirely on immunohistochemical staining. In this ...
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