Replicon cells
In Vitro RNA Replication Directed by Replicase Complexes Isolated from the Subgenomic Replicon Cells of Hepatitis C Virus
... NS5A) and possibly host factors. These additional components may play important roles in modulating polymerase activity, conferring template specificity and ensuring RNA synthesis with necessary fidelity during the ...
6
Dysfunction of Autophagy Participates in Vacuole Formation and Cell Death in Cells Replicating Hepatitis C Virus
... 1b replicon cells has been reported ...the replicon cells of genotype 1b, but not of geno- type 2a, were reduced in comparison with those in the cured cells (data not ...
10
Hepatitis C Virus Co-Opts Ras-GTPase-Activating Protein-Binding Protein 1 for Its Genome Replication
... in replicon cells in which HCV RCs have already been estab- ...lished. Replicon cells were transfected with duplex siRNAs targeting G3BP1 or a scrambled control duplex siRNA, and HCV RNA and ...
9
Rapid Intracellular Competition between Hepatitis C Viral Genomes as a Result of Mitosis
... that cells replicating two or more congenic HCV replicons decay over time into a single-replicon state and that this process is propelled by cellular ...dual-replicon cells to a ...
16
Hepatitis C Virus Induces the Localization of Lipid Rafts to Autophagosomes for Its RNA Replication
... Huh7-GFP-LC3 cells were solubilized and subjected to proteomics analysis for the characterization of their associated ...proteins. Replicon cells that did not express GFP-LC3 were also analyzed to ...
12
Hepatitis C Virus Replication Is Modulated by the Interaction of Nonstructural Protein NS5B and Fatty Acid Synthase
... HCV replicon cells and HCVcc-infected ...subgenomic replicon cell lysates was performed with an anti-FASN ...Huh7 cells were cotransfected with plasmids expressing FASN and NS5B (left) or FASN ...
11
Quantitative Proteomics Analysis Reveals BAG3 as a Potential Target To Suppress Severe Acute Respiratory Syndrome Coronavirus Replication
... genomic replicon and used it to screen inhibitors of SARS-CoV ...SARS-CoV replicon cells and parental BHK21 cells were compared using a quantitative proteomic strategy termed “stable-isotope ...
10
Stearoyl Coenzyme A Desaturase 1 Is Associated with Hepatitis C Virus Replication Complex and Regulates Viral Replication
... HEK293T cells were cotransfected with Flag-tagged SCD1 and each of Myc-tagged core, NS3, NS4B, NS5A, and ...Huh7.5 cells were infected with Jc1 for 4 h and then transfected with Flag-tagged SCD1 ...Huh6 ...
15
Dissecting the Interferon-Induced Inhibition of Hepatitis C Virus Replication by Using a Novel Host Cell Line
... HuH6 cells carries a mutation in the promoter region of that effector gene which would specifically affect its responsiveness to IFN- ␥ ...Huh-7 cells may express a similar or even identical set of ...
16
Replication of a hepatitis C virus replicon clone in mouse cells
... sequenced replicon clones obtained from our mouse cell lines to determine whether the population of sgJFH-1 replicons present in the MMH cells had acquired any adaptive ...MMH replicon cell lines ...
9
Inhibition of RIG-I-Dependent Signaling to the Interferon Pathway during Hepatitis C Virus Expression and Restoration of Signaling by IKKε
... Interferon (IFN) is one important effector of the innate immune response, induced by different viral or bacterial components through Toll-like receptor (TLR)-dependent and -independent mechanisms. As part of its ...
10
Control of Hepatitis C Virus Replication in Mouse Liver-Derived Cells by MAVS-Dependent Production of Type I and Type III Interferons
... miR122 cells engineered to express a replicating HCV luciferase replicon ...miR122 replicon cells (data not ...miR122 cells in the presence or absence of GFP or the HCV NS3-4A ...
13
Dissociation of a MAVS/IPS-1/VISA/Cardif-IKKε Molecular Complex from the Mitochondrial Outer Membrane by Hepatitis C Virus NS3-4A Proteolytic Cleavage
... HEK293 cells were cotransfected with 1 g of pcDNA3, Myc- ⌬ RIG-I, Myc-KIAA1271, Myc-KIAA1271(C508A), or Myc-TRIF and 2 g of IRF-3 ⌬ N, Flag-A20, or Flag–NS3-4A expression construct as ...
12
In Vitro and In Vivo Characterization of MicroRNA-Targeted Alphavirus Replicon and Helper RNAs
... in cells outside those used to generate ...with replicon RNA or copackaging of helper RNA (24, 31, 63, ...the replicon and helpers is low when using a two-helper RNA system (8, 10, 23, 24, 55, 62, ...
13
Persistent Replication of a Chikungunya Virus Replicon in Human Cells Is Associated with Presence of Stable Cytoplasmic Granules Containing Nonstructural Protein 3
... A home-built instant structured illumination microscope (iSIM) was used to acquire additional subdiffraction time-lapse series at high frame rates (one image every 88 ms) (Fig. 6A and B). This instrument is fitted with an ...
24
Inhibition of Interferon Signaling by the New York 99 Strain and Kunjin Subtype of West Nile Virus Involves Blockage of STAT1 and STAT2 Activation by Nonstructural Proteins
... Vero cells stably or transiently transfected with KUN or NY99 replicon ...Vero cells stably transfected with KUN replicon RNA (C20SDrepVero) ...KUN replicon RNA rep- lication and ...
9
Human La Protein Interaction with GCAC near the Initiator AUG Enhances Hepatitis C Virus RNA Replication by Promoting Linkage between 5′ and 3′ Untranslated Regions
... Huh7 cells were transiently cotransfected with wild-type or mutated RNA and the plasmid construct expressing ...Huh7 cells were cotransfected with either in vitro-transcribed wild-type or mutated HCV ...
14
Role of Cholesterol Pathways in Norovirus Replication
... in replicon-bearing ...HG23 cells up to 20 M, as determined by a cell cytotoxicity assay ...When replicon-bearing cells, HG23 cells, or G3 cells were treated with simvastatin ...
9
Expression of the Nucleoprotein of the Puumala Virus from the Recombinant Semliki Forest Virus Replicon: Characterization and Use as a Potential Diagnostic Tool
... Cloning the N gene in pSFV-1. The nucleoprotein gene was synthesized by RT-PCR using the RNA of Vero E6 cells infected with Puumala virus strain Cg 13891. Reverse transcription was performed with primer 5NPU ...
6
Repression of Interferon Regulatory Factor 1 by Hepatitis C Virus Core Protein Results in Inhibition of Antiviral and Immunomodulatory Genes
... in cells transfected with the plasmid expressing the HCV core protein than in cells transfected with the empty ...contrast, cells expressing the envelope proteins (E1 and E2) showed an increase in ...
13