Reverse line blot assay
Simultaneous Identification of 14 Genital Microorganisms in Urine by Use of a Multiplex PCR Based Reverse Line Blot Assay
... hybridization assay that permits the reliable, simultaneous detection of 14 known or potential urogenital pathogens, many of which are difficult to identify by other ...
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Reverse Line Blot Assay for Direct Identification of Seven Streptococcus agalactiae Major Surface Protein Antigen Genes
... This mPCR/RLB method has a number of advantages over our previous protein gene profiling method (12). It is easy to perform and does not require the use of gels; amplification products of 43 isolates can be tested ...
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Detection of Rickettsia in Rhipicephalus sanguineus Ticks and Ctenocephalides felis Fleas from Southeastern Tunisia by Reverse Line Blot Assay
... Species in the Rickettsia genus are divided into two groups: the spotted fever group (SFG) and the typhus group (TG). These ill- nesses are caused by about 22 species. Mediterranean spotted fever (MSF) is caused by ...
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Rapid detection of dermatophytes from skin and hair
... Very few studies have compared KOH microscopy and culture with direct PCR of clinical specimens In a case study, Nagao et al. detected Trichophyton rubrum by nested PCR targeting internal transcribed spacer gene 1 (ITS1) ...
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Performance of Commercial Reverse Line Blot Assays for Human Papillomavirus Genotyping
... the assay; and (iii) commercial availability in the United ...genotyping assay (LA) (Roche Diag- nostics, Indianapolis, IN), INNO-LiPA HPV Genotyping Extra (Innoge- netics, Ghent, Belgium), and the ...
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Replacing Reverse Line Blot Hybridization Spoligotyping of the Mycobacterium tuberculosis Complex
... spoligotyping assay involves the hybridization of amplified sample DNA to nylon membrane-immobilized oligonucleotides whose sequences are representative of 43 spacer ...spoligotyping assay using automated ...
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Nonisotopic Detection of Human Papillomavirus DNA in Clinical Specimens Using a Consensus PCR and a Generic Probe Mix in an Enzyme Linked Immunosorbent Assay Format
... immunosorbent assay format to screen for the presence of human papillomavirus (HPV) DNA amplified from clinical ...generic assay is performed, HPV DNA-positive samples can be directly genotyped using a ...
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Simultaneous Detection of BovineTheileria and Babesia Species by Reverse Line Blot Hybridization
... A reverse line blot (RLB) assay was developed for the identification of cattle carrying different species of Theileria and Babesia ...the assay, as well as parasites belonging to the ...
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Comparative study of bacteriological culture and real-time fluorescence quantitative PCR (RT-PCR) and multiplex PCR-based reverse line blot (mPCR/RLB) hybridization assay in the diagnosis of bacterial neonatal meningitis
... (mPCR)-based reverse line blot (RLB) hybridization assays are reliable and accurate tests which could increase the diagnostic yield of bacterial men- ingitis ...RT-PCR assay has characteristic ...
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New Method for Simultaneous Species Specific Identification of Equine Strongyles (Nematoda, Strongylida) by Reverse Line Blot Hybridization
... The accurate identification of horse strongyles irrespective of their life cycle stage is of paramount importance in increas- ing the understanding of their biology and epidemiology and the pathogenesis of associated ...
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Multiplex PCR Based Reverse Line Blot Hybridization Assay To Identify 23 Streptococcus pneumoniae Polysaccharide Vaccine Serotypes
... mead, New South Wales, Australia. Conventional serotyping was performed using the Quellung reaction, as previously de- scribed (3). Two isolates were not amplified by mPCR/RLB, and phenotypic retesting showed that they ...
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Is the Perceived Association between Chlamydia pneumoniae and Vascular Diseases Biased by Methodology?
... Inter- and intralaboratory inconsistencies in detection rates of Chlamydia pneumoniae in vascular specimens have been demonstrated. In this study, 66 vascular tissue specimens from 66 patients with vascular disease were ...
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Simultaneous Detection and Genotyping of “Norwalk Like Viruses” by Oligonucleotide Array in a Reverse Line Blot Hybridization Format
... Evaluation of the RLB assay. One hundred thirty-two NLV- positive stool samples from 34 OBs (112 samples) and 20 sporadic cases were used for validation of the RLB assay. Using the NLV RT-PCR followed by ...
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Nonisotopic Detection and Typing of Human Papillomavirus DNA in Genital Samples by the Line Blot Assay
... The line blot assay, a gene amplification method that combines PCR with nonisotopic detection of amplified DNA, was evaluated for its ability to detect human papillomavirus (HPV) DNA in genital ...a ...
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Simultaneous Detection and Identification of Candida, Aspergillus, and Cryptococcus Species by Reverse Line Blot Hybridization
... The major limitation of this assay and other molecular assays is time. A full working day is required for DNA extraction (relatively fixed for all assays, approximately 3 h), nested PCR (approximately 3 h), and ...
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Genotyping of Human Papillomavirus in Liquid Cytology Cervical Specimens by the PGMY Line Blot Assay and the SPF10 Line Probe Assay
... As stated in Materials and Methods, genotyping results were scored as concordant, compatible, and discordant. Since the two reverse hybridization methods use probes for different but overlapping ranges of HPV ...
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Rifampin Isoniazid Oligonucleotide Typing: an Alternative Format for Rapid Detection of Multidrug Resistant Mycobacterium tuberculosis
... the assay RIOT, which stands for rifampin-isoniazid oligonucleotide typing because it is based on reverse line blot DNA hybridization for the simul- taneous detection of mutations in genes ...
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Identification of 20 Common Human Enterovirus Serotypes by Use of a Reverse Transcription PCR Based Reverse Line Blot Hybridization Assay
... The complete VP1 genes of 37 isolates, providing one or two examples of each of our commonest 20 serotypes, were sequenced in two fragments by use of published primers (17). These were primer pairs 486-488 and 487-489 ...
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Use of PCR and Reverse Line Blot Hybridization Assay for Rapid Simultaneous Detection and Serovar Identification of Chlamydia trachomatis
... PCR-reverse line blot (RLB) hybridiza- tion assays for detection and serovar identification of Chlamydia ...RLB assay, labeled omp1 amplicons were hybridized to a membrane containing probes ...
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Bioelectronic DNA detection of human papillomaviruses using eSensor™: a model system for detection of multiple pathogens
... Five microliters of purified DNA from cytology samples was used in each PCR assay. HPV DNA was amplified with the PGMY09/PGMY11 primer system that uses a multi- plex format to amplify both HPV and β-globin in the ...
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