RNA FISH
Adventures With Rna Fish For Diagnosing Viral Infections And Exploring Single-Cell Heterogeneity In Cancer
... molecule RNA FISH assay to measure expression levels in many tens of thousands of individual cells to show that the primed state is characterized by rare-cell expression of high levels of resistance marker ...
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Silencing cuticular pigmentation genes enables RNA FISH in intact insect appendages
... Microinjections were performed using pulled borosilicate glass capillaries and a Nanoliter 2000 injector (both World Precision Instruments, Sarasota, FL, USA). Injection was parasagittally between the prothorax and ...
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BayFish: Bayesian inference of transcription dynamics from population snapshots of single molecule RNA FISH in single cells
... manufacturer’s instructions, which are available online. Custom Stellaris ® FISH Probes were designed against mouse Npas4 mRNA by utilizing the Stellaris ® RNA FISH Probe Designer (Biosearch ...
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flam piRNA precursors channel from the nucleus to the cytoplasm in a temporally regulated manner along Drosophila oogenesis
... RNA FISH on ovaries was performed as previously de- scribed [13]. In situ hybridization on OSS cells was car- ried out essentially described for ovaries. OSS cells were fixed in 4% formaldehyde/PBT (1X PBS, ...
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Measuring Transcription Directly From Our Chromosomes
... utilizes RNA fluorescence in situ hybridization[18, 62] (RNA FISH) in a way that allows us to uniquely identify and localize the transcriptional activity of 20 genes from the same chromosome in ...
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Non-coding RNA derived from a conservative subtelomeric tandem repeat in chicken and Japanese quail somatic cells
... performed FISH according to DNA/RNA hybridization protocol with single stranded oligonucleotide probes (PO41pos and PO41neg) to each strand of the ...repeat. RNA FISH was used as a reliable ...
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Kaposi's Sarcoma-Associated Herpesvirus mRNA Accumulation in Nuclear Foci Is Influenced by Viral DNA Replication and Viral Noncoding Polyadenylated Nuclear RNA
... Total RNA and genomic-DNA samples were taken at the point of fixation to ensure consistency between FISH slides (see below for processing of nucleic ...(maximum). FISH probes were labeled with ...
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Single Human Cells Use Transcriptional Mechanisms to Compensate for Differences in Cell Size and DNA Content
... FISH would be deemed “noisy”, or far from Poisson noise levels. We calculated CV 2 for mRNA counts as well as mRNA concentration (counts/volume) for many genes in our fibroblast cell line. Both of these measures ...
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Lack of sex chromosome specific meiotic silencing in platypus reveals origin of MSCI in therian mammals
... Cot-1 RNA FISH, cell enrichment, and RT-PCR to determine the status of sex chromosome specific transcriptional ...Cot-1 RNA FISH stained late prophase I nuclei that show obvious differences at ...
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Visualizing Allele Specific Expression In Single Cells
... Our bulk methylation analysis showed that a similar fraction of the mutant control regions are methylated as the fraction of mutant cells displaying monoallelic H19 expression, suggesting that variability in methylation ...
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Human telomerase RNA component (hTERC) gene amplification detected by FISH in precancerous lesions and carcinoma of the larynx
... ase RNA component (hTERC) gene, localized on chro- mosome 3q26, encodes the RNA component of human telomerase, and acts as a template for the addition of the repeat sequence ...
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MicroRNA-encoded behaviour in Drosophila
... other RNA-based regulatory processes affecting Hox gene expression might also impinge on specific neural outputs; we are currently investigating this possibility with especial regard to the roles of the Hox genes ...
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Using desktop hydrologic data to predict fish presence in streams in northern British Columbia
... is Fish and Fish Habitat, sometimes subsumed under a broader VC such as Freshwater Aquatic Resources (Stantec Con- sulting ...of fish habitat in streams which may be affected by a proposed ...on ...
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Up-regulation of circ_LARP4 suppresses cell proliferation and migration in ovarian cancer by regulating miR-513b-5p/LARP4 axis
... expression in OC cell lines was much lower than that in normal group. For the following observation, we sin- gled out two cell lines with lower expression of circ_ LARP4, SKOV3 and A2780. Besides, by treatment with ...
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ENHANCED EDGE DETECTION TECHNIQUES FOR IDENTIFICATION OF FISH THROUGH ITS MORPHOLOGICAL FEATURES
... The fish image can be classified using edge detection system that is proposed in this ...a fish image is built, which may be benefited by the various fields, the system concerning an isolated pattern of ...
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cDNA Sequence Analysis and Structural Phylogenetic Tree of Novel Myoglobin from Striped Snake-Head Fish (Ophicephalusstriatus)
... this fish in tolerance to low oxygen demand. Moreover, because the fish Mb contains two cysteine residues to form disulfide bond, it can covalently anchor within the tertiary structure to form a functional ...
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Current Status of Fish Waste Management In Karwar City
... Abstract: Fish waste management has a major problem with environmental and disposal of fish waste, due to rapid urbanization and Fish waste is high disposal ...of fish consumed, an equal ...
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Structure of Costs and Add Value to Small and Medium Enterprises in Agriproduct Processing Industry in Tabalong Regency, Indonesia
... Of all the agribusiness industry become the object of research, seen that the ground coffee processing business has the most value added ratio is large i.e. 92.11% while the value of the ratio of most small business is ...
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<p>Latest Advances of Long Non-Coding RNA SNHG5 in Human Cancers</p>
... The human genome-wide studies have demonstrated that approximately 70% of the human genome is transcribed into RNAs, while the ratio of non-coding RNAs (ncRNAs) is up to 98%. 1 The ncRNAs consist of long non-coding RNAs ...
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Lehmann, Elisabeth (2011): Structure-function analysis of RNA polymerase II: use of RNA as a template and site-directed mutagenesis in S. cerevisiae. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... lacking the non-essential subunit Rpb9 was shown to have similar intrinsic cleavage activities at mild alkaline conditions as wild-type Pol II (Weilbaecher et al., 2003). However, Rpb9 was implicated to play a role in ...
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