RNA I
Synchronous replication of poliovirus RNA: initiation of negative-strand RNA synthesis requires the guanidine-inhibited activity of protein 2C.
... virion RNA (6, 7, ...liovirus RNA and the assembly of infectious virus in a tempo- rally regulated ...preinitiation RNA replica- tion complexes form in HeLa S10 translation-RNA replication ...
8
Selective and Nonselective Packaging of Cellular RNAs in Retrovirus Particles
... two RNA species, we performed nondenaturing Northern analysis on viral ...SRP RNA signal was detected at the position of the dimeric genomic RNA, although as expected, a much more intense signal was ...
9
Complete replication of poliovirus in vitro: preinitiation RNA replication complexes require soluble cellular factors for the synthesis of VPg-linked RNA.
... of RNA replication com- plexes, the asymmetric replication of viral RNA, and the pro- duction of infectious ...viral RNA replication in the in vitro reactions were authentic relative to poliovirus ...
12
Perturbations at the ribosomal genes loci are at the centre of cellular dysfunction and human disease
... non-coding RNA produced from the intergenic spacer (IGS), which separates the rDNA ...noncoding RNA is produced in response to various stimuli including acid- osis, heat shock and transcriptional stress and ...
11
The matrix domain contributes to the nucleic acid chaperone activity of HIV 2 Gag
... HIV-1 RNA fragments and binds non-Ψ RNA with low specificity via its NC and MA domains, whereas bind- ing to Ψ RNA is highly specific and only the NC domain is engaged ...binds RNA more ...
15
Up-regulation of circ_LARP4 suppresses cell proliferation and migration in ovarian cancer by regulating miR-513b-5p/LARP4 axis
... LARP4 gene existed in some protists and all animals tested, while not in plants and yeasts [18]. LARP4 of mammal, which was also called LARP4A, had close inter- action with poly (A) RNA. It indicated that LARP4 ...
11
Visna virus RNA synthesis.
... We show that i uninfected cells do not contain RNA sequences related to the visna virus genome, ii parental RNA is rapidly transported to the nucleus of the infected cell, iii virus RNA [r] ...
8
Bacterial group I introns: mobile RNA catalysts
... group I intron RNA is by a two-step transesterification reaction with an exogenous GTP (αG) with its 3′–OH acting as an initiating nucleophile (Figure ...intron RNA by a 3′-5′ phosphodiester ...
12
RNA Polymerase I-Driven Minigenome System for Ebola Viruses
... Finally, we have confirmed that the packaging of REBOV minigenomes into progeny virions is possible within the mini- genome rescue system, based on the ability of supernatants from REBOV-infected cells that were ...
9
Hepatitis C Virus Nonstructural Protein 5A: Biochemical Characterization of a Novel Structural Class of RNA-Binding Proteins
... with RNA and to elucidate the minimal requirements ...(G/U) RNA shifts the equilibrium in favor of dimer; binding of other RNAs does not ...specific RNA binding (Fig. 6). This minimal RNA ...
12
Genome-Wide Approaches To Study Rna Secondary Structure
... and RNA has advanced rapidly, making transcriptome-wide expression profiling fast and widely ...dissertation, I discuss recent efforts to leverage this powerful technology to study, not just RNA ...
145
RNA-Dependent DNA Polymerase Activity of RNA Tumor Viruses I. Directing Influence of DNA in the Reaction
... Intfluence of Neurospora nuclease priming activity of DNA templatesa on dTMP incor- Expt Additions poration pmoles/ 30 min 1 Complete system deoxyribonuclease I-treated Complete system +[r] ...
14
RIG I ATPase Activity and Discrimination of Self RNA versus Non Self RNA
... Based on crystal structures of RIG-I with or without dsRNA and a non-hydrolyzable form of ATP, the transition from autore- pressed to activated RIG-I involves closure of its helicase domains upon binding ...
12
Translation and identification of the viral mRNA species isolated from subcellular fractions of vesicular stomatitis virus-infected cells.
... a Proteins of the virion; proteins synthesized in wheat germ extracts b in the absence of RNA and c in response to RNA from mock-infected cells; fraction I RNA d from membrane-bound poly[r] ...
8
Macromolecular Synthesis in Cells Infected by Frog Virus 3. VII. Transcriptional and Post-Transcriptional Regulation of Virus Gene Expression
... Our results clearly demonstrate that: i FV 3 RNA synthesis is controlled quantitativelynot all RNA species were synthesized in the same molar ratios; ii FV 3 RNA synthesis is controlled [r] ...
17
Crystallization of RNA-protein complexes I. Methods for the large-scale preparation of RNA suitable for crystallographic studies
... The third method described here overcomes this restriction and involves transcription of a plasmid template in which the gene encoding the desired RNA sequence is[r] ...
11
Sequential activation of splenic nuclear RNA polymerases by erythropoietin
... requirements and sensitivity to the fungal toxin alpha-amanitin, two major forms (I and II) of nuclear RNA polymerase were identified. Within 0.5 h after administration of erythropoietin, at a time when no ...
8
Activity-Dependent A-to-I RNA Editing in Rat Cortical Neurons
... extracted RNA and reverse-transcribed ...of RNA editing occurring at 25 rat exonic positions that are conserved and known to be edited in humans (Table ...standard RNA sequencing (RNA- seq), ...
20
Accurate Profiling of Gene Expression and Alternative Polyadenylation with Whole Transcriptome Termini Site Sequencing (WTTS-Seq)
... and RNA extraction: Three adult male and three adult female frogs (Xenopus tropicalis) ...total RNA extraction with Trizol reagent according to the manu- facturer’s ...total RNA with DNase (AM1906, ...
42
Identification of a Natural Viral RNA Motif That Optimizes Sensing of Viral RNA by RIG I
... well and grown overnight at 37°C. RNA FISH was performed according to published protocols, with minor modifications (48). At different times after transfection, the cells were washed with phosphate-buffered saline ...
11