RNA isolation
Phenol Based RNA Isolation is the Optimum Method for Study of Gene Expression in Human Urinary Sediment
... of RNA from this valuable source of biomarkers, mostly because of the factors that have influence on quality of isolated RNA such as low cellular ...of RNA from urine sediment samples that was ...
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A practical examination of RNA isolation methods for European pear (Pyrus communis)
... applicable RNA isolation methods in European pear fruit for downstream transcriptome analysis, we evaluated several commercially available kit-based RNA isolation methods, plus our modified ...
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RNA isolation method for single embryo transcriptome analysis in zebrafish
... Per RNA isolation, one ...The RNA was purified by column pre- cipitation according to the RNeasy MinElute Cleanup Handbook (version 2007) - Appendix D: RNA Cleanup after Lysis and ...
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An RNA isolation system for plant tissues rich in secondary metabolites
... isolate RNA or yielded negligible quantity of RNA from these plants (Figure ...the RNA iso- lation system ...the RNA isola- tion ...to RNA isolation, pos- sibly due to the ...
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Development of a simplified RT PCR without RNA isolation for rapid detection of RNA viruses in a single small brown planthopper (Laodelphax striatellus Fallén)
... without RNA isolation for RNA virus detection in a single ...virus RNA can be prepared in approximately two ...with RNA isolation, the simplified RT-PCR method greatly reduces ...
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A novel technique for isolating DNA from Tempus™ blood RNA tubes after RNA isolation
... the RNA isolation step, so hereafter we share our developed pro- ...(lacking RNA) which included gDNA, denatured protein, the stabilizing solution, and ...DNA isolation protocol ...
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RNA isolation for transcriptomics of human and mouse small skin biopsies
... skin RNA-isolation ...of RNA extraction [1,2], are available in our setting, we decided to adapt our previously described robust and highly-efficient protocol for RNA extraction from single ...
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Comparison of different methods for DNA free RNA isolation from SK N MC neuroblastoma
... extracted RNA with a low level of polysaccharides ...highest RNA concentration and yield was obtained by the AxyPrep Multisource Total RNA Miniprep kit while RNeasy ® Mini kit, EasySpin kit and ...
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Simultaneous DNA and RNA isolation from brain punches for epigenetics
... and RNA yields and absorbance rates for all meth- ods studied are listed in Table ...Puregene isolation Kits (both 360 ...tested RNA methods, the Chomczynski protocol yielded the highest amount of ...
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Development of a Protocol to Measure Gene Expression in the Mouse Tibia
... of RNA isolation, reverse transcription, and PCR were completed ...the RNA isolation, homogenizing the tissue and extracting RNA was the most difficult ...of RNA, but ...
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Highly efficient method for isolation of total RNA from adipose tissue
... total RNA isolation from adipose tissue, the miR- Neasy method results in cleaner samples but more prone to degradation and the TRI Reagent W method results in samples contaminated with salts and solvents ...
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Comparison of different methods for preparation and characterization of total RNA from cartilage samples to uncover osteoarthritis in vivo
... quent isolation method. For example, milling before RNA isolation with TRIzol® reagent and RNeasy ™ resulted in a significant RNA degradation [see Figure 1 and Additional files 1 and ...the ...
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Isolation and detection of small RNA molecules
... Total RNA isolation. Homogenize fresh plant material (4 g of leaves, seedlings, etc.) in liquid nitrogen with a mortar and a pestle. Transfer ho- mogenate into RLT buffer (2 ml/g, Qiagen) in 50-ml falcon ...
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Isolation of high-quality RNA from a wide range of woody plants
... ABSTRACT: Isolation of high-quality RNA is one of the most crucial methods in molecular ...biology. RNA extraction from woody plants has been problematic due to the presence of rigid and woody ...
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Comparison of RNA extraction kits and histological stains for laser capture microdissected prostate tissue
... of RNA isolated with the RNeasy (median RIN ...quality RNA and led to suc- cessful RNA isolation of every ...isolate RNA from microdissected ...tested RNA extraction ...extracted ...
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Comparison of commercial RNA extraction kits for preparation of DNA free total RNA from Salmonella cells
... commercial RNA extraction kits was evaluated: RiboPure-Bacteria Kit (Ambion, Inc), PureLink RNA Mini Kit (Invitrogen), UltraClean Micro- bial RNA Isolation Kit (MoBio), RNeasy Mini Kit (QIA- ...
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Lagos Bat Virus in Kenya
... Bats were collected by hand nets or manually in the caves and human dwellings and mistnetted around roosts or in locations of nocturnal foraging. Both adult and subadult animals (based on body size) were randomly ...
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Limited Protection from a Pathogenic Chimeric Simian-Human Immunodeficiency Virus Challenge following Immunization with Attenuated Simian Immunodeficiency Virus
... Differential nef RNA PCR assay. A differential PCR assay was developed to determine the presence of a full nef sequence. The assay was designed to include a pair of primers that bind outside of the 59 and 39 ends ...
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Molecular Cloning and Functional Analysis of IRF-1 in the Asian Swamp Eel (Monopterus albus)
... However, data on immune genes of M. albus are scarce compared to other commercially important fishes. Therefore, a better understanding of the immune response of M. albus may contribute to develop strategies for disease ...
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Isolation of poliovirus 2C mutants defective in viral RNA synthesis.
... This result indicates that the 2C protein accumulated during infection at the permissive temperature in 2C-31-infected cells could maintain viral RNA synthesis for only a short period af[r] ...
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