RNA polymerase binding site
Mutagenesis Analysis of the rGTP-Specific Binding Site of Hepatitis C Virus RNA-Dependent RNA Polymerase
... rGTP-specific binding site on cell colony formation induced by HCV RNA ...catalyze RNA polymerization on both HCV-specific RNA and nonviral RNA templates (2, 11, 13, 17, 21, ...
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The Crystal Structure of Coxsackievirus B3 RNA-Dependent RNA Polymerase in Complex with Its Protein Primer VPg Confirms the Existence of a Second VPg Binding Site on Picornaviridae Polymerases
... for binding a po- tential drug may vary significantly between RdRps of the same viral family and even the same ...active site is en- circled and sits in the center, to which the substrates have access ...
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RNA Binding of Ebola Virus VP30 Is Essential for Activating Viral Transcription
... start site (8, 17). We propose that VP30 clamps the RNA template and VP35/L together to increase the polymerase’s affinity for the ( ⫺ ) RNA template, either to prevent the enzyme from dissociation ...
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Replication of Poliovirus Requires Binding of the Poly(rC) Binding Protein to the Cloverleaf as Well as to the Adjacent C-Rich Spacer Sequence between the Cloverleaf and the Internal Ribosomal Entry Site
... and RNA transfection. Prior to transcrip- tion by T7 RNA polymerase, pT7PV1(M) and its derivatives were linearized with EcoRI, whereas PV-Luc and its derivatives were linearized with ...The ...
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Role of Second-Largest RNA Polymerase I Subunit Zn-Binding Domain in Enzyme Assembly
... The following multistep procedure was used to create the rpa135⌬Zn mutant, lacking the Zn-binding domain. In the first step, two PCRs were performed to amplify RPA135 regions at the left and the right sides of the ...
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Mutation of lysine residues in the nucleotide binding segments of the poliovirus RNA-dependent RNA polymerase.
... nucleotide binding sites in the poliovirus RNA poly- ...all RNA-dependent RNA ...among RNA-templated polymerases, but its location within the three- dimensional structure of the enzyme ...
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Differential activation of the influenza virus polymerase via template RNA binding.
... negative-strand RNA virus, with each segment packaged into a viral ...viral polymerase, along with the ribonucleoprotein, is required for initiating ...The polymerase complex comprises three protein ...
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Cross-Linking of the Fingers Subdomain of Human Immunodeficiency Virus Type 1 Reverse Transcriptase to Template-Primer
... (PAGE). Polymerase and RNase H ...template RNA was synthesized according to the instructions contained in the Ambion Megashortscript kit (Am- bion, Austin, ...primer binding site (PBS) ...
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Binding of ICP4, TATA-Binding Protein, and RNA Polymerase II to Herpes Simplex Virus Type 1 Immediate-Early, Early, and Late Promoters in Virus-Infected Cells
... reduced binding of ICP4, TBP, and polII to the promoter region. The binding of TBP to the DS1 region was also de- tected, but it was approximately 20% of that detected at the ...TBP binding to the tk ...
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Binding mechanism of remdesivir to SARS-CoV-2 RNA dependent RNA polymerase
... the RNA/NTP binding site were mutated from SARS-CoV to ...ATP/remdesivir binding with SARS- CoV-2 NSP12 based on a previous co-crystal structure of poliovirus ...relative binding free ...
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The Crystal Structure of a Cardiovirus RNA-Dependent RNA Polymerase Reveals an Unusual Conformation of the Polymerase Active Site
... bose binding pocket toward the entrance of the rNTP ...the polymerase N ...cardiovirus RNA replication process and may have important implications for the development of new antivirals targeting the ...
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Mutation of large T-antigen-binding site A, but not site B or C, eliminates stalling by RNA polymerase II in the intergenic region of polyomavirus DNA.
... To determine whether binding of large T antigen to viral DNA is required to block elongation by RNA polymerase II, we examined the level of RNA polymerase stalling on the late strand of [r] ...
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Association of the Influenza A Virus RNA-Dependent RNA Polymerase with Cellular RNA Polymerase II
... that RNA polymer- ase II (Pol II) transcription and RNA processing are coupled in vivo (11, 12, ...and RNA processing are influenced not only by cellular factors such as CTD kinases and phosphatases ...
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Mycobacterial RNA Polymerase Requires a U Tract at Intrinsic Terminators and Is Aided by NusG at Suboptimal Terminators
... mycobacterial RNA polymerase requires U-tracts for intrinsic termination, which can be aided by NusG, will guide future study of mycobacte- rial transcription and aid improvement of predictive algorithms to ...
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Endurance training lowers ribosome density despite increasing ribosome biogenesis markers in rodent skeletal muscle
... protein, RNA pol I protein and pre-45S rRNA expression patterns, the expression of select genes involved with rRNA pro- cessing and ribosome assembly (Nop56 and Ncl) were greatest in exercised ...
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Ribonucleic Acid Transcriptases in Sendai Virions and Infected Cells
... In this paper, we report that the mammalian paramyxovirus, Sendai virus, also contains an RNA polymerase, and we compare the virion enzyme with the RNA polymerase previously described in[r] ...
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Evidence for activation of the hepatitis B virus polymerase by binding of its RNA template.
... substrate RNA, and (ii) the ε sequences are located 3 9 to the P open reading frame in the TYDP con- ...the RNA that contains the differences between them is destroyed prior to the start of the trans ...the ...
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Activation of 2′ 5′ oligoadenylate synthetase by stem loops at the 5′ end of the West Nile virus genome
... genomic RNA secondary ...stranded RNA-activated protein kinase (PKR), 29 59 -oligoadeny- late synthetases (OAS) and adenosine deaminase acting on RNAs (ADARs) ...dsRNA binding results in a ...
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Sequence in the Influenza A Virus Nucleoprotein Required for Viral Polymerase Binding and RNA Synthesis
... RT-PCR. RNA was TRIzol extracted from a dual-luciferase reporter assay in which the positive-sense firefly luciferase RNA (cRNA) served as the ...The RNA was then treated with 1 U of RNase-free DNase ...
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Isolation and Mapping of Small Cauliflower Mosaic Virus DNA Fragments Active as Promoters in Escherichia coli
... This is in part due to the small differences between the binding of eucaryotic RNA polymerase to specific, correct pro673 Downloaded from http://jvi.asm.org/ on November 10, 2019 by gues[r] ...
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