RNA replication and transcription
Role of the Virus Nucleoprotein in the Regulation of Lymphocytic Choriomeningitis Virus Transcription and RNA Replication
... LCMV transcription and replication are equally enhanced by increasing intracellu- lar NP levels is consistent with the reported NP-mediated enhancement of polymerase processivity in SeV ...genome RNA ...
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Mechanism of coronavirus transcription: duration of primary transcription initiation activity and effects of subgenomic RNA transcription on RNA replication.
... This model is consistent with most previously described observations; subgenomic DI RNA synthesis does not occur in subgenomic DI RNA-transfected, MHV-infected cells 20, subgenomic RI RN[r] ...
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RNA Replication without RNA-Dependent RNA Polymerase: Surprises from Hepatitis Delta Virus
... RNA-dependent RNA replication is a special process re- served exclusively for RNA viruses but not cellular ...all RNA viruses (except retroviruses) undergo RNA- dependent ...
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A Nucleolar Protein, Ribosomal RNA Processing 1 Homolog B (RRP1B), Enhances the Recruitment of Cellular mRNA in Influenza Virus Transcription
... either transcription or replication on the same vRNA ...of transcription and replication in influenza A virus infections have been ...its RNA binding, which might modify the pan- handle ...
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Brome Mosaic Virus Polymerase-Like Protein 2a Is Directed to the Endoplasmic Reticulum by Helicase-Like Viral Protein 1a
... positive-strand RNA virus in the alphavirus-like superfamily, encodes RNA replication proteins 1a and ...viral RNA capping, and 2a contains a central polymerase-like ...(ER)-associated ...
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Analysis of the Kinetics of Transcription and Replication of the Rotavirus Genome by RNA Interference
... the replication cycle of the ...vitro replication assays and through the char- acterization of the phenotype of temperature-sensitive mu- ...viral RNA in a cell-free system using ...
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Requirements for the self-directed replication of flock house virus RNA 1.
... T7 RNA polymerase from the plasmid FHV1[2,12], which have 2 extra 5 9 nucleotides and 12 extra 3 9 nucleotides beyond the authentic FHV 1 sequence, underwent both translation to yield functional RNA ...
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Transcription of Hepatitis Delta Antigen mRNA Continues throughout Hepatitis Delta Virus (HDV) Replication: a New Model of HDV RNA Transcription and Replication
... HDV RNA polyadenylation signal, originally reached by Hsieh and Taylor, who examined the effect of HDAg expression on an HDV cDNA polyadenylation signal ...HDV RNA was required for HDAg suppression of ...
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The Nucleolus Is the Site of Borna Disease Virus RNA Transcription and Replication
... viral RNA synthesis occurs in the nucleolus, the presence of certain BDV proteins would be required in the ...viral RNA replication since genomic and antigenomic viral RNAs of NNS viruses are en- ...
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Individual expression of influenza virus PA protein induces degradation of coexpressed proteins.
... single-stranded RNA segments of negative polarity, en- coding a total of 10 genes (for a review, see reference ...where transcription and replication take place (13). Transcription initiation ...
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Ssrp1a controls organogenesis by promoting cell cycle progression and RNA synthesis
... damage response. Indeed, increased Tp53-target gene expression (Vogelstein et al., 2000) (supplementary material Fig. S4H-K) and a partial rescue of cell death in ssrp1a;tp53 mutants (supplementary material Fig. S4F,G) ...
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La Crosse Virus Nonstructural Protein NSs Counteracts the Effects of Short Interfering RNA
... as RNA interference (RNAi), double-stranded short interfering RNAs (siRNAs) silence gene expression in a sequence-specific ...of transcription, we designed 13 synthetic siRNAs targeting specific RNA ...
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Measles virus synthesizes both leaderless and leader-containing polyadenylated RNAs in vivo.
... Transcription and replication of measles virus RNA might have different protein requirements, since late in infection cycloheximide reduced leader-containing and antigenomic RNAs but not[r] ...
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Characterization of a temperature-sensitive defect of enterovirus 70: effect of elevated temperature on in vitro transcription.
... Thus block of single-stranded RNA synthesis and accumulation of RF in viral transcription, as seen in EV70 replication at the nonpermissive temperature, might be a phenomenon rather freq[r] ...
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Trimethylation of Histone H3 Lysine 4 by Set1 in the Lytic Infection of Human Herpes Simplex Virus 1
... Thus, it appears that HSV-1 is at least partially in a nucleo- somal state during acute infection and that alternative histone modifications decorate the HSV-1 genome during lytic and latent infection (10, 11, 14, 15, ...
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Initiation of Genomic Plus-Strand RNA Synthesis from DNA and RNA Templates by a Viral RNA-Dependent RNA Polymerase
... disrupted, RNA replication was greatly reduced (18), whereas disruption of the putative minus-strand stem-loop structure was not correlated with effects on viral replication ...plus-strand RNA ...
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Functional Analysis of the Complex trans-Activating Response Element RNA Structure in Simian Immunodeficiency Virus
... Tat-TAR transcription activation mechanism was functionally replaced by the DOX-inducible Tet-On gene regulation system ...activate transcription from the downstream transcription initiation ...site. ...
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Conservative replication and transcription of Saccharomyces cerevisiae viral double-stranded RNA in vitro.
... Our present experiments demonstrate for the first time that replication synthesis of minus strand occurs in virus particles in vitro and that ScV transcription and replication are conser[r] ...
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Genetic Interaction of Flavivirus Nonstructural Proteins NS1 and NS4A as a Determinant of Replicase Function
... The RNA replication of YFDSKden isolates was compared to that of YF 17D and YFDSK on YF NS1-, DEN NS1-, and GFP-expressing cells by examination of the accumulation of viral plus strands at 16 h ...
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gkp904.pdf
... for RNA synthesis, as previously reported for the nsp9-RdRp of the arterivirus equine arteritis virus (EAV), a distant relative in the order Nidovirales ...an RNA template in the presence of Mn 2+ ...
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