RT-PCR
Superiority of Digital Reverse Transcription PCR (RT PCR) over Real Time RT PCR for Quantitation of Highly Divergent Human Rhinoviruses
... conventional PCR using the type-specific T7 polymerase promoter-labeled forward primer and type-specific reverse ...for RT-qPCR and 1e2 to 1e6 copies/reaction for RT-dPCR) were amplified using the ...
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Quantitative real-time RT-PCR of CD24 mRNA in the detection of prostate cancer
... real-time RT-PCR for specific and sensitive detection of CD24 transcripts as a supplementary measure for discriminating between malignant and benign lesions in prostatic ...
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DEVELOPMENT OF MOLECULAR DIAGNOSIS METHOD FOR IDENTIFICATION OF HCV USING LAMP AND RT PCR TECHNIQUES
... Tube discarded. A new Collection Tube was kept. 450 µl Wash Buffer was added to the upper reservoir of the Filter Tube and centrifuged for 1 min at 8,000 × g and the flowthrough was discarded. After the first wash and ...
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Hot start reverse transcriptase: an approach for improved real-time RT-PCR performance
... Real-time RT-PCR is the method of choice in molecular diagnostics for detection and quantification of defined RNA molecules (Mackay 2004). This technique utilizes reverse transcriptase (RT) to ...
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Real time RT PCR high resolution melting curve analysis and multiplex RT PCR to detect and differentiate grapevine leafroll associated virus 3 variant groups I, II, III and VI
... Real-time RT-PCR amplicons of GLRaV-3 variant groups I, II, III and VI were cloned into a pGEM-T-easy Vector (Promega) and sequenced to obtain variant-specific plasmid ...DNA PCR HRM assays contained ...
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Identification of an EWS-pseudogene using translocation detection by RT-PCR in Ewing's sarcoma.
... Hence EWS should not be used as an internal control for the RNA quality in a RT-PCR based test for the presence of the tranSlOCation.. O 1995 Academic Press, I n c.[r] ...
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Detecting Newcastle disease virus in combination of RT PCR with red blood cell absorption
... regular RT-PCR in treating varied samples and different NDV ...the RT- PCR and red blood cell adsorption is useful when the amount of NDV particles is too low to be detected during the onset ...
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Diagnosis of Rabies via RT PCR on Skin Samples of Wild and Domestic Animals
... To reduce the potential risk of saliva contamination of the skin, carcasses were thoroughly cleaned under run- ning water. A 2 - 4 cm 2 section of skin was removed from the muzzle area, using a fresh sterile blade and ...
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Molecular Detection of Hepatitis C Virus (HCV) by Conventional One-step RT-PCR Coupled with Nested PCR
... One-Step RT-PCR assay, initially RT (conversion of RNA into cDNA) step was performed after that heat inactivation of RT enzyme was done followed by simple PCR ...nested PCR was ...
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Protocol: a highly sensitive RT-PCR method for detection and quantification of microRNAs
... stem-loop RT primer designed accord- ing to Chen et ...pulsed RT reaction. Next, the RT product was amplified using a miRNA-specific forward primer and the universal reverse primer (Figure ...
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The detection of Gram negative bacterial mRNA from soil by RT-PCR
... The aim of this study was to develop a quick and reliable technique to extract RNA of a quality suit- able for RT-PCR from a clay-rich soil. To determine the e¡ectiveness of this technique, soil seeded with ...
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Molecular detection of Subfamily Pneumovirinae among Children with Flu-like illness by using RT-PCR
... CAAGTGTGACATTGCTGAYCTRAA -ʹ3), reverse primer (5ʹ-ACTGCCGCACAACAACATTTAGRAA-ʹ3) and Probe (5ʹ-TGGCYGTYAGCTTCAGTCAATTCAACAGA -ʹ3) [23]. The probe for hMPV uses FAM dye on the 5' reporter end and a non- fluorescent dye on ...
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One step real time RT PCR assays for serotyping dengue virus in clinical samples
... serotype-specific RT-PCR assays pre- sented here were able to successfully serotype dengue in clinical ...DENV2 RT-PCR assays were found to be more sensitive than the corresponding ...
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Stable hepatitis C virus RNA detection by RT PCR during four days storage
... All the samples stored at room temperature or stored at 4 ° C for 6, 24, 48, 72, and 96 hours were positive for the quantitative RT-PCR. The mean viral load was 362,000 IU/ml (5.544 log;SD = 0.023) for ...
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Airborne Rhinovirus Detection and Effect of Ultraviolet Irradiation on Detection by a Semi-Nested RT-PCR Assay
... The majority of rhinoviral transmission among adults is thought to occur via airborne infectious droplet nuclei [8– 10]. This study was conducted to assess the ability to de- tect airborne rhinovirus on air sampling ...
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Establishment and Application of Digital RT PCR Assay for Detection of Avian Influenza Virus H9 Subtype
... digital PCR tests, droplets and chip. Unlike traditional quantitative PCR techniques, dPCR is independent of the circula- tory threshold (CT) of the amplification curve and is not affected by amplifica- ...
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Metabolic regulation analysis of an ethanologenic Escherichia coli strain based on RT-PCR and enzymatic activities
... Total RNA extraction was performed using hot-phenol equilibrated with water, precipitated with 3 M sodium acetate and ethanol, and treated with DNase kit (DNA- free™, Ambion; [33]. RNA integrity was tested by densit- ...
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Reference gene identification for reliable normalisation of quantitative RT-PCR data in Setaria viridis
... standard RT-PCR approach and the construction of melt ...via RT-PCR analysis ...via RT-qPCR analysis across 52 tissue sam- ples ...by RT-PCR with all eleven sequences ...
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Identification, by differential display RT-PCR and cDNA selection, of genes expressed in human testis
... The starting material for Differential Display RT-PCR can be either total or poly(A)" RNA. Partial cDNA fragments are reverse transcribed from the mRNA using an anchored oligo-dT primer mixture; this ...
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Application of in situ reverse trancriptase-polymerase chain reaction (RT-PCR) to tissue microarrays
... quantitative RT-PCR analysis of gene expression [13,14] was carried out on first-strand cDNA derived from RNA isolated from samples of normal and tumour ...Each PCR reaction contained 10 ng ...
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