RT-PCR, reverse transcriptase polymerase reaction
Heminested reverse transcriptase polymerase chain reaction (hnRT PCR) as a tool for rabies virus detection in stored and decomposed samples
... rogen) and ultra pure water in a final volume of 50 μL. The amplification was performed on a MJ Research PCT- 200 Thermal Cycler. The following cycling conditions were adopted: initial heating at 95°C/3 min, 35 cycles at ...
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COMPARING THE GENES OF Artemisia annua AND Artemisia ludoviciana WITH RESPECT TO THE ARTEMISININ BIOSYNTHETIC PATHWAY
... the reverse transcriptase polymerase chain reaction (RT-PCR) were subjected to an agarose gel electrophoresis to visualize amplicons obtained against a molecular weight marker ...
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A comparative analysis of routine techniques: Reverse transcriptase polymerase chain reaction (RT-PCR) and five cell lines for detection of enteroviruses in stool specimens
... However, RT-PCR on pre-treated stool specimens could detect 55 Enteroviruses in 230 specimens (24%); much higher than Enterovirus detection rate in cell ...
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Magnetic Nanowire Networks for Dual-Isolation and Detection of Tumor-Associated Circulating Biomarkers
... Next, we attempted to verify tumor-specific genomic mutations in CTCs by ddPCR (Figure 5d). In general, genomic DNA (gDNA) from CTCs is often more accessible and reliable for gene expression profiling than cfDNA, as DNA ...
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Multiplex reverse transcriptase pcr assay for the detection of infective stage wuchereria bancrofti and its vector culex quinquefasciatus
... There is currently a global program attempting to eliminate lymphatic filariasis (LF) by administering drugs to affected communities with the goal of interrupting transmission of the parasite. At present the c ...
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Application of in situ reverse trancriptase-polymerase chain reaction (RT-PCR) to tissue microarrays
... quantitative RT-PCR analysis of gene expression [13,14] was carried out on first-strand cDNA derived from RNA isolated from samples of normal and tumour ...Each PCR reaction contained 10 ng ...
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Gene expression induced by interleukin 17 in fibroblast like synoviocytes of patients with rheumatoid arthritis: upregulation of hyaluronan binding protein TSG 6
... kinase; RT–PCR = reverse transcriptase– polymerase chain reaction; RA = rheumatoid arthritis; SFC = fibroblast-like synoviocytes; TBST = tris-buffered saline containing Tween 20; ...
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A report on Japanese encephalitis in Mizoram: clinical investigation and diagnosis
... However, reverse transcriptase polymerase chain reaction (RT-PCR) based methodologies have resulted in increased sensitivity for the detection of JEV viruses in clinical samples, ...
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Eye Findings in Infants With Suspected or Confirmed Antenatal Zika Virus Exposure
... with reverse transcriptase polymerase chain reaction (RT-PCR)– positive and unconfirmed case patients who were born during and soon after an outbreak period is used to justify ...
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MOLECULAR ANALYSIS OF MLL GENE REARRANGEMENTS IN CHILDHOOD ACUTE LEUKEMIA: A HOSPITAL BASE STUDY
... multiplex reverse-transcriptase polymerase chain reaction (PMRT-PCR) technique was performed for molecular ...Quantitative RT-PCR assay of MLL revealed a down regulation ...
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Skeletal muscle expression of creatine kinase-B in end-stage renal disease
... mRNA RT-PCR amplification product in one of seven skeletal muscle samples ...to RT-PCR analysis in only 7 of 45, because of lack of specimen availabil- ...No RT-PCR amplification ...
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Osteoblastic differentiation and stress response of human mesenchymal stem cells exposed to alternating current electric fields
... Universal PCR Master Mix (Applied Biosystems, Foster City, CA) and the ABI Prism 7000 Sequence Detection System (Applied ...Biosystems). RT-PCR deter- mined mRNA expression of osteogenic ...
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Identification of Spata-19 New Variant with Expression beyond Meiotic Phase of Mouse Testis Development
... Total RNA was extracted from the cell lines and testis tissues using Tri-Pure isolation reagent (Molecular Bioscience) according to the manufacturer’s instructions. RNA was dissolved in DEPC-treated water and its ...
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Epidemiological and Genetic Overview of the Klebsiella pneumoniae Carbapenemases (KPCs) in K. pneumoniae Isolated from the Clinical Samples in Iran
... A reaction mixture, consisting of 5 µg of RNA, was incubated in DEPC-treated water and 1 µL of random hexamers (Pars Tous, ...of RT premixture (2X) was added, it was mixed by pipetting up and down gently in ...
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Microarray profile of differentially expressed genes in a monkey model of allergic asthma
... use RT-PCR to validate microarray results, most of the primers and probes in RT-PCR are designed on the basis of human sequences, which in fact works well on monkey ...
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The influence of micrometastases on prognosis and survival in stage I-II colon cancer patients: the Enroute⊕ Study
... [3-5] Focused examination methods, such as more extensive nodal examination by serial sectioning or step sectioning, or molecular detection of metastatic nodal cells by immunohistochemistry (IHC) or reverse tran- ...
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Altered serum microRNAs as biomarkers for the early diagnosis of pulmonary tuberculosis infection
... qRT- PCR (Applied Biosystems). Assays were performed using the RT stem-loop primer, PCR primers and ...probes. RT reactions were performed using the TaqMan miRNA Re- verse Transcription Kit ...
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A New Sensitive Method for Detection of Viroids
... acid. RT-PCR-DBH assay for viroid detection have two levels of specificity based on RT-PCR primers and an internal hybridization ...probe. RT-PCR-DBH assay with the specificity, ...
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Serum osteopontin: a biomarker of disease activity and predictor of relapsing course in patients with giant cell arteritis Potential clinical usefulness in tocilizumab treated patients
... and reverse-tran- scribed to cDNA using Archive Kit (Applied Biosys- tems, Life Technologies) in a final volume of 100 µL, employing random hexamer ...Real-Time PCR System and results were analysed with the ...
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Identification and characterization of a novel efficient resistance response to the furoviruses SBWMV and SBCMV in barley
... Previous studies suggested that P. graminis ribotype I may preferentially infect barley and transmit bymoviruses such as Barley yellow mosaic virus and Barley mild mosaic virus, whereas P. graminis ribotype II may ...
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