Serial dilution
A Serial Dilution Study of Gadolinium Based MR Imaging Contrast Agents
... To this end, we designed an in vitro serial dilution study of each of the 5 FDA-approved GBMCAs currently available for clinical practice in the United States. These solutions would be simultaneously imaged ...
6
Isolation and identification of concrete environment bacteria
... The bacteria strains were successfully isolated and enrichment process was conducted. The bacteria was purified by conducting serial dilution streaking plate. The pure strain of bacteria obtained was ...
10
Enhanced fermentative performance under stresses of multiple lignocellulose-derived inhibitors by overexpression of a typical 2-Cys peroxiredoxin from Kluyveromyces marxianus
... stresses. Serial dilution assay indicated that KmTPX1 gene contributed to a better cel- lular growth behavior, when the cells were exposed to multiple lignocellulose-derived inhibitors, such as formic acid, ...
13
Characterization of a Novel MDH1 Bacterium from a Virgin Hot Spring Applicable for Gold Nanoparticle (GNPs) Synthesis
... Background: Use of novel microorganisms for beneficial purposes is still remaining a challenging job. This study was designed to isolate, characterize and use of a novel hot spring bacterial strain from a virgin hot ...
10
PREVALENCE OF CONTAMINATING MICROORGANISMS IN ANTI MALARIAL DRUGS SOLD IN CALABAR, CROSS RIVER STATE, NIGERIA
... Preparation of syrups: A ten-fold serial dilution was carried out to reduce the microbial load and 0.1ml was plated onto different agar medium (Nutrient, MacConkey and Sabouraud Dextrose agar) in sterile ...
6
Isolation of Pure Culture of Bacteria From Soil and Study their Antimicrobial Activity
... Soil contains different types of microorganisms including bacteria actinomycets, fungi, protozoa, algae, and viruses. The aim of present study was undertaken to isolate specific bacteria from soil and convert it into ...
6
Microbial Observation in Bioaccumulation of Heavy Metals from the Ash Dyke of Thermal Power Plants of Chhattisgarh, India
... Different bacteria were isolated from the Ash Dyke area of four different plants situated in Chhattisgarh, using serial dilution technique in nutrient medium. Since nutrient medium is the most familiar ...
9
Quantification of Intestinal Bacterial Populations by Real Time PCR with a Universal Primer Set and Minor Groove Binder Probes: a Global Approach to the Enteric Flora
... a serial dilution of bacterial DNA from different strains showed that the detection limits of MGB probes for both the VIC-labeled universal probe and FAM-labeled specific probes were 2 log ranks higher than ...
7
A Validated Stability Indicating RP-HPLC Method Development for Platelet Aggregation Inhibitor Ticagrelor in Bulk and Tablet Formulation
... For photolysis condition, 100 mg of the pure drug was weighed and transferred to a clean petri-dish and closed. Then the petri-dish was exposed to direct sunlight. At different timings, 10mg of drug sample was taken out. ...
7
Synthesis and pharmacological evaluation of 6 hydroxy 2,5,7,8 tetramethyl N' (2 oxoindolin 3 ylidene)chroman 2 carbohydrazide derivatives as antimicrobial agents
... A series of new 6-hydroxy-2,5,7,8-tetramethyl-N'-(2-oxoindolin-3-ylidene)chroman-2-carbohydrazide derivatives (4-10) were synthesized with moderate to higher yields. The in vitro antimicrobial activity of these compounds ...
6
Enhanced detection of neoantigen-reactive T cells targeting unique and shared oncogenes for personalized cancer immunotherapy
... peptide pools (Figure 2D). Both IFN-γ secretion and upregulation of T cell activation markers assessed by flow cytometry demonstrated that each TIL culture was reactive solely against 1 potential neoan- tigen. Also, by ...
17
The development and use of Actiphage® to detect viable mycobacteria from bovine tuberculosis and Johne’s disease-infected animals
... Phage D29 suspensions were titred on a lawn of M. smegmatis grown in soft Middlebrook 7H10 agar (0.75 % w/v) and incubated at 37°C for 18 h. The phage amplification assay and experimental controls were car- ried out ...
9
Accuracy and Precision of Quantitative Calibrated Loops in Transfer of Bronchoalveolar Lavage Fluid
... the serial dilution method and the calibrated loop method ...the serial dilution method, ...two serial 100-fold dilutions are inoc- ulated onto the agar plate ...the dilution ...
5
EVALUATION OF IPOMOEA SETOSA CRUDE TITER EXTRACT EFFECT ON NAJA PHILIPPINENSIS MICE INDUCED VENOM TOXICITY
... in serial dilution of 1:60, 1:100 and 1:120 titers of Ipomoea setosa crude seed extract that exerted a counter toxic effect against cobra venom induced toxicity test in 30 ICR laboratory ...
9
Transmission of Human T-Cell Leukemia Virus Type 1 to Mice
... Sensitivity of detection of HERV-R, pX, gag, and LTR se- quences by PCR. We checked the sensitivity of detection of pX and HERV-R sequences by PCR-Southern blot analysis using a serial dilution of MT-2 cell ...
6
Comparison of various detection methods for periodontopathic bacteria: can culture be considered the primary reference standard?
... The present research was initiated to compare the ability of several detection methods, i.e., a serial dilution anaerobic culture and/or microscopic procedure 21, a DNA probe procedure 2[r] ...
9
Synthesis, Spectral Characterization and Antimicrobial Studies of New Hybrid Heterocyclic Compounds Bearing 1H-benzimidazol-2-yl Thiomethyl Motif
... First, 200 μl of sterile deionised water was added to all outer perimeter wells of sterile 96 wells plate to minimize the evaporation of medium in the test wells during incubation. The wells received 100 μl of the ...
10
Analytical biodegradation of Azo dye (Remazol red RB) by Bacillus cereus
... The bacterial isolates present in the textile dye effluent were isolated by serial dilution technique and spread plate method. The plates were incubated at 37°C for 24 hours. After incubation, the bacterial ...
7
GRANULES OF UNISTRAIN LACTOBACILLUS AS NUTRACEUTICAL ANTIOXIDANT AGENT
... The intact cells of formulation 1 and 2 exhibited the scavenging DPPH radical by 27.50 and 23.99%, respectively. The results also showed that intact cells and cell-free extract of two formulations exhibited obviously ...
6
Thesis
... the dilution series should be approached to determine antibody level to specific proteins of the influenza ...the serial dilution series completed for this project, the data could be re- analyzed to ...
38