single-particle cryo-electron microscopy
Structure, assembly and dynamics of macromolecular complexes by single particle cryo-electron microscopy
... Macromolecular complexes are therefore at the center of most biological processes. They integrate spatially several catalytic or structural activities with built-in reg- ulatory functions. In most of the cases, ...
8
3D structure of individual nanocrystals in solution by electron microscopy
... a single object at many different tilt angles over 2 to 5 hours, assuming the object is static during the entire ...acquisition. Single particle cryo-electron microscopy ...
22
Cryo electron Microscopy Structure of the Acinetobacter baumannii 70S Ribosome and Implications for New Antibiotic Development
... use single-particle cryo-electron microscopy (cryo-EM) to elucidate five different structural states of the ...inter- particle motions of the 70S ribosome in different tRNA ...
12
A national facility for biological cryo electron microscopy
... for single-particle analysis and tomography can also be used for recording electron ...was electron crystallography of two-dimensional crystals, particularly of membrane proteins (Henderson ...
10
Collaborative computational project for electron cryo microscopy
... The first CCP-EM training event will cover the maximum- likelihood single-particle reconstruction program RELION being developed at the MRC-LMB (Scheres, 2012). The program uses an empirical Bayesian ...
5
Electron Cryo-Microscopy and Single-Particle Averaging of Rift Valley Fever Virus: Evidence for GN-GC Glycoprotein Heterodimers
... using electron cryo-tomog- raphy revealed five- and six-coordinated glycoprotein capsom- ers organized on a T ⫽ 12 lattice ...a single structural unit composed of both G N and G C forms both ...
8
Cryo-electron microscopy structure of yeast Ty retrotransposon virus-like particles.
... STEM mass-radius analysis indicates an icosahedral T- number series. Figure 1 shows a STEM image of p1-381 Ty- VLPs used in the mass-radius analysis. These particles have had their packaged RNA removed by RNase ...
6
Recent advances in retroviruses via cryo electron microscopy
... through cryo-electron microscopy that can be of aid in the field of ...that Cryo-EM and related techniques can be seen throughout all fields related to cellular ...While electron ...
10
Approaches to altering particle distributions in cryo-electron microscopy sample preparation
... of cryo-EM users plunge freezing has remained the only method of preparing macromolecular complexes for single-particle ...alter particle distribution and thin-film formation across a grid ...
12
Cryo electron microscopy to determine the structure of macromolecular complexes
... by electron tomography in which a series of views of the same area are collected over the whole range of tilts ...accumulated electron dose and the missing data due to limited tilt, the resolution is ...
9
The structures of a naturally empty cowpea mosaic virus particle and its genome-containing counterpart by cryo-electron microscopy
... positive-sense, single-stranded RNA ...a single RNA-2-encoded precursor polyprotein (VP60) by the action of the 24 K viral proteinase which is encoded by ...
10
Computational Challenges in High Resolution Cryo Electron Microscopy
... In a second, related example, we recorded multiple, iterative images of fields of frozen hemocyanin particles using 10 electrons/Å 2 for each image. As more and more images were recorded, the structure of the particles ...
120
Multivariate Statistical Analysis of Large Datasets: Single Particle Electron Microscopy
... to single particle cryo-EM known as the “maximum likelihood” [61], an “explanatory factor analysis” ...in single-particle cryo-EM and in depth comparisons will be performed in ...
39
Cryo-Electron Microscopy Structure of Seneca Valley Virus Procapsid
... Negative-stain single-particle reconstruction of pentamers. The procapsid sample at pH 6.0 from the previous section was used for further imaging and image reconstruction. Briefly, 36 micrographs were ...
15
A structural investigation of bacterial twin arginine translocation (tat) complexes by single particle electron microscopy
... A TatBC complex with multiple substrates bound to its periphery has been previously observed by EM (Tarry et al., 2009). A central cavity was shown in this complex; however, it was deemed too small to accommodate a Tat ...
291
Neyer, Simon (2017): RNA polymerase I complex structures elucidate mechanisms of transcription initiation and elongation. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... Samples were prepared and cryo-EM data was collected as described (Engel et al., 2016). Apart from particles with disordered stalk and clamp domains, we observed particles with dissociated A49-A34.5 subcomplex, ...
121
Assessment of in vitro particle dosimetry models at the single cell and particle level by scanning electron microscopy
... fluorescence microscopy can be performed within a few seconds per image covering hundreds of cells [41], sample preparation and imaging by SEM coupled with semi-automatic NP quantification takes up to a week, and ...
15
The U4 Antibody Epitope on Human Papillomavirus 16 Identified by Cryo-electron Microscopy
... H uman papillomavirus (HPV) infections continue to be a sig- nificant health burden in patient populations (1, 2). Al- though commercial vaccines targeting the viral capsid proteins have been applied successfully to ...
10
Cryo-Electron Microscopy Structure of Lactococcal Siphophage 1358 Virion
... the cryo-electron microscopy reconstruction of a unique lactococcal phage that possesses genomic similarity to particular Listeria phages and has a host range restricted to only a minority of ...
11
DEVELOPMENT AND CHARACTERIZATION OF SOLID LIPID NANOPARTICLES BY SOLVENT DIFFUSION EVAPORATION METHOD FOR TOPICAL DELIVERY
... For particle size and polydispersity index measurement, the diluted nanoparticles dispersion was poured into the cuvette which was placed in the cuvette holder of the instrument and analyzed using the zetasizer ...
7