Single strand conformation polymorphism analysis
Identification of Mycobacterium Species by Multiple Fluorescence PCR–Single Strand Conformation Polymorphism Analysis of the 16S rRNA Gene
... sequent single-strand conformation polymorphism (SSCP) analysis (MF-PCR-SSCP) of four variable regions of the 16S rRNA gene were used to identify species-specific patterns for 30 of the ...
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Novel Algorithm Identifies Species in a Polymycobacterial Sample by Fluorescence Capillary Electrophoresis Based Single Strand Conformation Polymorphism Analysis
... FCE-RF-SSCP analysis of 19 mycobacterial species and ...FCE-RF-SSCP analysis of 16S-23S rDNA ITS fragments amplified from artificially constructed mycobacterial ...
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Molecular identification of bacteria by fluorescence based PCR single strand conformation polymorphism analysis of the 16S rRNA gene
... Automated SSCP detects the ssDNA during electrophoresis and makes optimal use of the separation capacity of the SSCP gel, and it is therefore suitable for multiplex PCR-SSCP. Mul- tiplex PCR-SSCP (15) makes a further ...
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Multiple infections of Ixodes scapularis ticks by Borrelia burgdorferi as revealed by single strand conformation polymorphism analysis
... The amplified segments from ticks which were infected with a single mobility class were chosen for DNA sequencing. DNAs from two isolates of each mobility class were sequenced. The polymorphic sites are listed in ...
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Intensive Linkage Mapping in a Wasp (Bracon hebetor) and a Mosquito (Aedes aegypti) With Single-Strand Conformation Polymorphism Analysis of Random Amplified Polymorphic DNA Markers
... RAPD-SSCP analysis revealed segregation of codominant alleles at markers that appeared to segregate as dominant (band presence/band absence) markers or appeared invar[r] ...
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Rapid Identification of Bacteria from Positive Blood Cultures by Fluorescence Based PCR–Single Strand Conformation Polymorphism Analysis of the 16S rRNA Gene
... Bacteremia continues to result in significant morbidity and mortality, particularly in patients who are immunocompromised. Currently, patients with suspected bacteremia are empirically administered broad- spectrum ...
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Genetic Diversity among Borrelia Strains Determined by Single Strand Conformation Polymorphism Analysis of the ospC Gene and Its Association with Invasiveness
... Wang et al. (24) have shown that the genetic diversity of B. burgdorferi sensu stricto within a local population of ticks is almost equal to the worldwide genetic diversity. Our study revealed that ospC groups identified ...
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Detection of rifampin resistance by single strand conformation polymorphism analysis of cerebrospinal fluid of patients with tuberculosis of the central nervous system
... a single copy of the rpoB gene is present in myco- bacterial DNA, PCR protocols with this target are less sensi- tive than those amplifying repeated sequences, such as ...
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Simple Method for Determining Biovar and Serovar Types of Ureaplasma urealyticum Clinical Isolates Using PCR–Single Strand Conformation Polymorphism Analysis
... PCR-SSCP analysis were not performed on identical ...PCR-SSCP analysis did not detect any mixed groups among these specimens, al- though mixtures of serovars are commonly encountered on ...a single ...
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Characterization of Salmonella Serovars by PCR Single Strand Conformation Polymorphism Analysis
... PCR-RFLP analysis indicate that there are differences in the HaeIII re- striction sites within the groEL genes of strains belonging to different serovars and strains within a given ...
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Capillary Electrophoresis–Single Strand Conformation Polymorphism Analysis for Rapid Identification of Pseudomonas aeruginosa and Other Gram Negative Nonfermenting Bacilli Recovered from Patients with Cystic Fibrosis
... We evaluated 2 to 4% polymer and 5 to 10% glycerol. The best results were obtained with 4% polymer and 10% glycerol. Perkin-Elmer recommends purifying PCR products by remov- ing salts and excess primers with Amicon ...
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Hereditary renal amyloidosis with a novel variant fibrinogen
... a polymorphism in fibrinogen A alpha chain gene by single-strand conformation polymorphism analysis, and affected individuals from two kindreds were found to have a ...
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Ghrelin alleles and use of the same for genetically typing animals
... Single-Strand Conformation Polymorphism Analysis The identi?cation of a DNA sequence linked to Ghrelin can be made Without an ampli?cation step, based on poly morphisms including restric[r] ...
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Genotypic detection of Mycobacterium tuberculosis rifampin resistance: comparison of single strand conformation polymorphism and dideoxy fingerprinting
... Cost analysis of ddF versus automated DNA sequencing. The approximate material cost and time commitment for doing a ddF reaction (5 9 -end fluorescein- labelled primer, with products analyzed by using an automated ...
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Detection of Rifampin Resistant Mycobacterium tuberculosis in Sputa by Nested PCR Linked Single Strand Conformation Polymorphism and DNA Sequencing
... PCR-SSCP analysis. Furthermore, nested PCR- SSCP analysis enabled the direct detection of rifampin resis- tance from primary clinical specimens, such as ...
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Typing of Pneumocystis carinii f sp hominis by single strand conformation polymorphism of four genomic regions
... Among the 12 BAL specimens from the 11 Swiss patients investigated in the present study, only a few polymorphic po- sitions were observed in the four regions analyzed. Moreover, most of these positions and many of the ...
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DETECTION SINGLE NUCLEOTIDE POLYMORPHISMS IN UROMODULIN PROMOTER REGION ASSOCIATED WITH RENAL DISEASES USING SINGLE STRAND CONFORMATION POLYMORPHISM POLYMERASE CHAIN POLYMORPHISMS TECHNIQUE
... The amplified fragments were purified using the protocol suggested by Macrogen sequencing company (Macrogen - Korea), and then, they were sequenced using each primer pair. The sequences of the gene fragments analyzed ...
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Molecular analysis of patient and carrier genes with congenital steroid 21 hydroxylase deficiency by using polymerase chain reaction and single strand conformation polymorphism
... caused by point mutations of the CYP21B (CYP21A2) gene, whereas the remaining 20% are due to deletion of this gene, we used the polymerase chain reaction single strand conformation ...
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A single strand conformation polymorphism study of CD40 ligand Efficient mutation analysis and carrier detection for X linked hyper IgM syndrome
... Linkage analysis suggested that she had inherited the same alleles at CD40 ligand as her affected ...SSCP analysis of exon 2 demonstrated that although the pa- tient’s mother and one of his sisters were ...
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Sequence Variations in the Bovine Growth Hormone Gene Characterized by Single-Strand Conformation Polymorphism (SSCP) Analysis and Their Association with Milk Production Traits in Holsteins
... Our objective was to screened the entire length of the bovine GH gene for sequence variations and study the associa- tions of changes in potential GH sequence variants with ch[r] ...
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