Single Tube Multiplex PCR
Single-tube multiplex PCR using type-specific E6/E7 primers and capillary electrophoresis genotypes 21 human papillomaviruses in neoplasia
... a single microfuge tube 19 definite or probable HR-HPV genotypes (HPV types 16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 70, 73, 82) [8,9] and in addition low-risk (LR) HPV6 and 11 and as ...
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Development and evaluation of one step single tube multiplex RT PCR for rapid detection and typing of dengue viruses
... both multiplex RT-PCR and two step RT-PCR followed by nested PCR, which revealed 100% concordance (Details not ...the multiplex RT-PCR assay was compared with closely related ...
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Evaluation of a Single Tube Multiplex Real Time PCR for Differentiation of Members of the Mycobacterium tuberculosis Complex in Clinical Specimens
... real-time PCR assay for the differentiation of ...a single-tube five-plex real-time PCR assay to differentiate these ...real-time PCR were tested, resulting in a 94% correlation of the ...
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Cost Effective Real Time Reverse Transcriptase PCR (RT PCR) To Screen for Dengue Virus followed by Rapid Single Tube Multiplex RT PCR for Serotyping of the Virus
... When virus detection fails, clinicians usually resort to serol- ogy tests for the detection of specific antibodies to dengue virus. Various serological detection methods have been de- scribed (1, 17, 19, 22). The ...
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Rapid Identification of Nine Microorganisms Causing Acute Respiratory Tract Infections by Single Tube Multiplex Reverse Transcription PCR: Feasibility Study
... the PCR-ELISA system from Boehringer ...of PCR product, and the product was denatured by adding 25 ml of ...as PCR positive or negative according to a cutoff OD value of ...specific single ...
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Path-ID Multiplex One-Step RT-PCR Kit
... ™ Multiplex One-Step RT-PCR Kit is designed for multiplex, quantitative, reverse transcription PCR ...a single-tube, one-step ...
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Development and Clinical Evaluation of an Internally Controlled, Single Tube Multiplex Real Time PCR Assay for Detection of Legionella pneumophila and Other Legionella Species
... Diagnostic PCR assays have principally targeted specific regions within 16S rRNA genes (rDNA) (3, 9, 12, 21, 23, 26), 5S rDNA (9), or the macrophage inhibitor potentiator (mip) gene (1, 9, 15, 16, 17, 20, 22, ...
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Typing of Dengue Viruses in Clinical Specimens and Mosquitoes by Single Tube Multiplex Reverse Transcriptase PCR
... general, PCR-based techniques can be more rapid, sensitive, and specific than alternative techniques when they are made accessible by a low-cost methodology that involves the in-house preparation of reagents and ...
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One Step, Multiplex, Real Time PCR Assay with Molecular Beacon Probes for Simultaneous Detection, Differentiation, and Quantification of Human T Cell Leukemia Virus Types 1, 2, and 3
... A single-tube, multiplex, real-time PCR assay with molecular beacons was established in which various probes were used for the simultaneous detection, differentiation, and quantification of ...
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Simultaneous Detection of Neisseria meningitidis, Haemophilus influenzae, and Streptococcus pneumoniae in Suspected Cases of Meningitis and Septicemia Using Real Time PCR
... A single-tube 5 ⴕ nuclease multiplex PCR assay was developed on the ABI 7700 Sequence Detection System (TaqMan) for the detection of Neisseria meningitidis, Haemophilus influenzae, and ...
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A multiplex one tube nested real time RT PCR assay for simultaneous detection of respiratory syncytial virus, human rhinovirus and human metapneumovirus
... multiple PCR and mono RT-qPCR assays have been routinely applied in the well-equipped laboratory for the diagnosis of RSV, HRV and ...Multiple PCR assays [8, 13] enable detection of co-infection but are ...
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Identification of Listeria Species by Microarray Based Assay
... one-tube multiplex PCR amplification of six target bacterial virulence factor genes (iap, hly, inlB, plcA, plcB, and clpE), synthesis of fluorescently labeled single-stranded DNA, and ...
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Comparison of three multiplex PCR assays for the detection of respiratory viral infections: evaluation of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder SMART 22 assay
... novel multiplex PCR methods, the RespiFinder-19 assay, RespiFinder-SMART(Single tube Multiplex Amplification in Real-Time)-22 assay (both PathoFinder, Maastricht, Netherlands) and the ...
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Comparison of Multiplex PCR-ELISA and conventional Multiplex PCR for detection of HIV-1/HCV co-infection
... of multiplex assays (18). Although multiplex PCR simplifies the procedures and reduces the need for time, final analysis remains tiresome, expensive and time consuming (1, ...in-house ...
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Molecular identification of Clonorchis sinensis and discrimination with other opisthorchid liver fluke species using multiple Ligation depended Probe Amplification (MLPA)
... as: single adult was ground in a 1.5 ml microcentrifuge tube con- taining 200 μ l of extraction buffer I, after shortly homoge- nizing, proteinase K(New England Biolabs, ...
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Application of Multiplex PCR for Detection and Differentiation of Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii.
... highlighted. Multiplex PCR is a molecular bi- ology technique for amplification of multiple targets in a single PCR ...tiplex PCR was used extensively for pathogen identification, ...
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Genotyping of Toxoplasma gondii Isolates with 15 Microsatellite Markers in a Single Multiplex PCR Assay
... by PCR using fluorescently labeled forward and unlabeled reverse ...a multiplex PCR assay with 5 MS markers that were able to reach the typing level but not the fingerprinting level of discrimination ...
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Multiplex Real Time PCR shortTUB Assay for Detection of the Mycobacterium tuberculosis Complex in Smear Negative Clinical Samples with Low Mycobacterial Loads
... Finally, eight of the positive culture samples tested negative for both targets in the assay. In two of these cases, only the solid culture (Coletsos medium; bioMérieux Clinical Diagnostics, Spain) was positive, after ...
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Simultaneous Detection and Typing of Influenza Viruses A and B by a Nested Reverse Transcription PCR: Comparison to Virus Isolation and Antigen Detection by Immunofluorescence and Optical Immunoassay (FLU OIA)
... A nested reverse transcription (RT)-PCR was developed for simultaneous detection and typing of influenza viruses A and B. The detection limit for influenza virus A subtypes H1 and H3 and that for influenza virus B ...
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Single tube, nested, reverse transcriptase PCR for detection of viable Mycobacterium tuberculosis
... a PCR technique which could distinguish viable from nonviable tubercle ...DNA-targeted PCR was incapable of determining mycobacterial viability over the time period ...positive PCR signal 4 weeks ...
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