spa TYPING
spa Typing of Staphylococcus aureus as a Frontline Tool in Epidemiological Typing
... The typing data resulting from those studies can also be used to enlarge our knowledge of spa-MLST mappings, which is extremely useful for the daily routine typing of ...“reference spa types” ...
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Validation of Pulsed Field Gel Electrophoresis and spa Typing for Long Term, Nationwide Epidemiological Surveillance Studies of Staphylococcus aureus Infections
... by spa typing with strains belonging to the same CC but to dif- ferent STs which harbored the same spa ...similar spa profiles which clustered in a unique spa CC (spa ...the ...
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spa Typing Method for Discriminating among Staphylococcus aureus Isolates: Implications for Use of a Single Marker To Detect Genetic Micro and Macrovariation
... Strain typing of microbial pathogens has two major aims: (i) to index genetic microvariation for use in outbreak investigations and (ii) to index genetic macrovariation for use in phylogenetic and population-based ...
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Assignment of Staphylococcus Isolates to Groups by spa Typing, SmaI Macrorestriction Analysis, and Multilocus Sequence Typing
... by spa typing and other typing techniques (15, 22, 29, ...of spa typing revealed a considerable de- gree of spa gene repeat polymorphism within particular clonal groups and ...
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Comparing Pulsed Field Gel Electrophoresis with Multilocus Sequence Typing, spa Typing, Staphylococcal Cassette Chromosome mec (SCCmec) Typing, and PCR for Panton Valentine Leukocidin, arcA, and opp3 in Methicillin Resistant Staphylococcus aureus Isolates at a U S Medical Center
... Each MRSA isolate underwent characterization by PFGE, using SmaI as a restriction endonuclease, at the Minnesota Department of Health (3). Patterns were compared by using Dice coefficients, and those that were within 80% ...
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Evaluation of spa-typing of methicillin-resistant Staphylococcus aureus using high-resolution melting analysis
... additional spa-types (Table ...a spa-type. Taken all together, we conclude that HRM- Spa typing is useful due to its reproducibility, simplicity, rapid and low ...aureus ...
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Comparison of Multiple Locus Variable Number Tandem Repeat Analysis with Pulsed Field Gel Electrophoresis, spa Typing, and Multilocus Sequence Typing for Clonal Characterization of Staphylococcus aureus Isolates
... of typing Staph- ylococcus aureus, was compared to pulsed-field gel electrophoresis (PFGE), spa typing, and multilocus sequence typing (MLST) on a group of 59 ...by spa typing ...
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Genotyping of Methicillin Resistant Staphylococcus aureus Strains from Two Hospitals in Bangalore, South India
... MLST and spa typing. MLST was performed as previously described by En- right et al. (8). Internal fragments of the seven housekeeping genes for carbam- ate kinase (arcC), shikimate dehydrogenase (aroE), ...
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Analysis of Typing Methods for Epidemiological Surveillance of both Methicillin Resistant and Methicillin Susceptible Staphylococcus aureus Strains
... ferent typing methods over time. PFGE has been the standard typing technique during this period due to its high discrimina- tory power and relatively low cost per ...of spa typing and MLST ...
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Clonality and antimicrobial susceptibility of methicillin-resistant Staphylococcus aureus at the University Hospital Zurich, Switzerland between 2012 and 2014
... and spa-typing is more discriminatory as compared to using a single method only and will allow to better monitor and recog- nize changes in MRSA epidemiology over time, iii) clonal complex predictions based ...
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Evaluation of Protein A Gene Polymorphic Region DNA Sequencing for Typing of Staphylococcus aureus Strains
... RFLP typing with the gene probes mecA and Tn554 required separate electrophoretic equipment in addition to a transfer apparatus and labeling products for the ...compare typing information for the original ...
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Typing of Methicillin Resistant Staphylococcus aureus in a University Hospital Setting by Using Novel Software for spa Repeat Determination and Database Management
... Strains. MRSA strains were isolated and identified from various clinical spec- imens sent to the Institute for Hygiene and Microbiology at the University of Wu¨rzburg. Only patients and staff members of the Wu¨rzburg ...
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Global distribution and diversity of ovine associated Staphylococcus aureus
... sequence typing (MLST) and spa ...87 spa types in the 293 ovine isolates; in the 97 new ovine isolates there were 22 STs and 37 spa types, including three novel MLST alleles, four novel STs ...
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Enhanced Discrimination of Highly Clonal ST22 Methicillin Resistant Staphylococcus aureus IV Isolates Achieved by Combining spa, dru, and Pulsed Field Gel Electrophoresis Typing Data
... and spa types. This study investigated whether combining PFGE and spa typing with DNA sequencing of the staphylococcal cassette chromosome mec element (SCCmec)-associated direct repeat unit (dru ...
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Classifying spa Types in Complexes Improves Interpretation of Typing Results for Methicillin Resistant Staphylococcus aureus
... that spa typing is associated with speed, low costs, and comparability of data, spa typing was used in this study for typing of MRSA ...ferent spa types were found, of which the ...
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DNAGear a free software for spa type identification in Staphylococcus aureus
... A spa typing application has been developed to be freely available to the academic purposes. It finds sequence types, new types, and new repeats in the Staphylococcus aureus sequences. Furthermore, it has a ...
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Emergence of Hospital and Community Associated Panton Valentine Leukocidin Positive Methicillin Resistant Staphylococcus aureus Genotype ST772 MRSA V in Ireland and Detailed Investigation of an ST772 MRSA V Cluster in a Neonatal Intensive Care Unit
... on spa typing, all of the isolates apart from one were assigned to spa type ...to spa type t345, which differs in one repeat unit only from spa type ...
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Characterization of SCCmec, spa types and Multi Drug Resistant of methicillin resistant Staphylococcus aureus isolates among inpatients and outpatients in a referral hospital in Shiraz, Iran
... Results: Total of 159 S. aureus isolates were collected from two tertiary hospitals in Shiraz. Isolates were identified by biochemical tests. Antimicrobial susceptibility tests were performed by standard disk diffusion ...
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Predominance and Emergence of Clones of Hospital Acquired Methicillin Resistant Staphylococcus aureus in Malaysia
... MRSA typing methods are not suited to detailed epidemiological surveillance (20, ...ular typing methods can be used for supporting infection con- trol and for tracing the nosocomial sources and transmission ...
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Whole Genome Sequencing for High Resolution Investigation of Methicillin Resistant Staphylococcus aureus Epidemiology and Genome Plasticity
... did spa typing, as evidenced by its identification of 17 groups compared to the 12 groups resolved by spa typing ...either spa typing or MLVA revealed that substantial numbers of ...
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