SsDNA binding
A Phage Single-Stranded DNA (ssDNA) Binding Protein Complements ssDNA Accumulation of a Geminivirus and Interferes with Viral Movement
... of ssDNA in protoplasts and in plants but not for systemic infection and symptom development in ...of ssDNA and increased amounts of double-stranded DNA (dsDNA), compared to accumulation in the presence of ...
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Analysis of the XPA and ssDNA-binding surfaces on the central domain of human ERCC1 reveals evidence for subfunctionalization.
... Truncation studies of ERCC1 have mapped the XPA interaction site to a region between residues 91 and 118 (34). From the cERCC1 structure and its compact fold, we predict that such truncations will have a devastating ...
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Processivity Clamp gp45 and ssDNA-Binding-Protein gp32 Modulate the Fidelity of Bacteriophage RB69 DNA Polymerase in a Sequence-Specific Manner, Sometimes Enhancing and Sometimes Compromising Accuracy
... coated with gp32, thus increasing the ability of gp44/62 in the presence of four different combinations of acces- to compete with gp43 in binding to the 3⬘ end of the sory proteins: none, gp32 (either alone or in ...
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A structural analysis of DNA binding by hSSB1 (NABP2/OBFC2B) in solution
... to ssDNA by X-ray crystallog- raphy. Binding to ssDNA was found to be exclusively medi- ated by the OB domain of ...that ssDNA recognition by hSSB1 within the SOSS1 com- plex is achieved ...
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Structural and functional analysis of the nucleotide and DNA binding activities of the human PIF1 helicase
... conserved ssDNA binding channel in hPIF1 that we show is critical for single-stranded DNA binding during unwinding, but not the binding of G quadruplex ...the ssDNA-binding ...
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Structural and functional analysis of the nucleotide and DNA binding activities of the human PIF1 helicase
... direct ssDNA binding assays (Fig- ure ...in ssDNA binding and helicase activity de- scribed ...the ssDNA binding channel resulted in variant hPIF1 forms with signif- icantly ...
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Spatiotemporally Different DNA Repair Systems Participate in Epstein-Barr Virus Genome Maturation
... Previous cross-sectional studies of EBV replication com- partments by confocal laser microscopy demonstrated EBV replication proteins to be localized at viral replication com- partments, also revealing variation in ...
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Role of the Hydrophilic Channels of Simian Virus 40 T-Antigen Helicase in DNA Replication
... ssDNA binding activity of mutant T ...bind ssDNA. This was tested in an ssDNA binding assay using a 55-mer labeled oligonucleotide in the presence of ...normal ssDNA ...
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Helicase Motif Ia Is Involved in Single-Strand DNA-Binding and Helicase Activities of the Herpes Simplex Virus Type 1 Origin-Binding Protein, UL9
... the ssDNA-binding activity of UL9, a ssDNA- binding assay was ...contact ssDNA, would exhibit little or no defect in ssDNA ...contact ssDNA are expected to exhibit defects ...
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Role of ATP hydrolysis in the DNA translocase activity of the bovine papillomavirus (BPV-1) E1 helicase
... DNA- binding properties of a helicase protein derived from the C-terminal domain of BPV ...with ssDNA or ATP but not ADP or AMP–PNP. The minimal DNA-binding site size for efficient oligomerization is ...
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VirE2: A Unique ssDNA Compacting Molecular Machine
... of ssDNA from its cytoplasm to the host plant cell ...translocated ssDNA and mediate ssDNA ...protein—VirE2—enhances ssDNA transfer efficiency ...transferred ssDNA (T-DNA) and forms a ...
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Rescue of Bacteriophage T7 DNA Polymerase of Low Processivity by Suppressor Mutations Affecting Gene 3 Endonuclease
... Compensatory evolution of T7 phage. The self-sufficient ge- nome organization of bacteriophage T7 and its robust lytic life cycle have made it an attractive model to investigate genetic adaptation under environmental ...
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Rad51 Protein from the Thermotolerant Yeast Pichia angusta as a Typical but Thermodependent Member of the Rad51 Family
... TMD buffer and 2.0 mM ATP with its regenerating system for 5 min at 37°C. Then, E. coli ssDNA-binding protein and yeast RPA were added to RPA con- centrations of 6 and 3 M, respectively. After 7 min, the ...
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Nucleotide sequence of the genome of Pf3, an IncP-1 plasmid-specific filamentous bacteriophage of Pseudomonas aeruginosa.
... In the Ff phages and IKe, the ssDNA-binding protein gene gene V and the major coat protein gene gene VIII are separated by two small genes genes VII and IX encoding minor coat proteins w[r] ...
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Roles of Replication Protein-A Subunits 2 and 3 in DNA Replication Fork Movement in Saccharomyces cerevisiae
... Replication Protein-A, the eukaryotic SSB, consists of a large subunit (RPA1) with strong ssDNA binding activity and two smaller subunits (WAS and 3) that may coope[r] ...
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Structure of the hDmc1 ssDNA filament reveals the principles of its architecture
... The helical ‘‘thread’’ of DNA density inside of the nucleoprotein filaments has diameter of ,18A ˚ and 30A˚ in extended and compressed forms respectively. The extended and compressed states have protomer density ...
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Single-strand DNA Binding by the Helix-Hairpin-Helix Domain of XPF Contributes to Substrate Specificity of ERCC1-XPF
... DNA binding experi- ments demonstrated a clear preference of XPF for ssDNA sub- strates ...Most ssDNA sequences tested were suitable substrates for XPF irrespective whether XPF was present as ...
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Distinct clonotypes of anti DNA antibodies in mice with lupus nephritis
... Clonotypes of IgG anti-DNA antibodies were studied by isoelectric focusing in various autoimmune mice with or without lethal lupus nephritis. MRL/MpJ-lpr/lpr mice exhibited the most heterogeneous spectrotypes of anti-DNA ...
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Plasma virome of cattle from forest region revealed diverse small circular ssDNA viral genomes
... circular ssDNA virus from Rhesus macaque (KU043429 and KU043430) [32] and four porcine stool- associated circular viruses (KJ577812-KJ577815) [33], sharing 52% sequence similarity with ...
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Binding dynamics of a monomeric SSB protein to DNA : a single molecule multi process approach
... the ssDNA of A647- SsoSSB dimers pre-formed in solution or if the second monomer binds to the ssDNA at a faster rate than our time- resolution (33 ms), or a combination of ...
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