Subgenomic replicon
Effect of Hepatitis C Virus (HCV) NS5B-Nucleolin Interaction on HCV Replication with HCV Subgenomic Replicon
... wild-type replicon was represented by MA, in which S232 of NS5A was altered to I, because this mutant replicon can efficiently replicate in Huh7 cells (36, ...the replicon MA/cm211, each of the amino ...
9
Inhibition of the foot-and-mouth disease virus subgenomic replicon by RNA aptamers.
... a subgenomic replicon incorporating GFP (pGFP-PAC replicon), allowing replication to be monitored and quantified in ...of replicon-bearing cells with a 3D pol aptamer RNA resulted in ...
9
Hepatitis C Virus Subgenomic Replicon Requires an Active NS3 RNA Helicase
... Con1 subgenomic replicon to ascertain the role of the helicase in viral ...new replicon lacked two-thirds of the NS3 helicase ( ⌬ hel), and six others contained one of the following six amino acid ...
8
In Vitro RNA Replication Directed by Replicase Complexes Isolated from the Subgenomic Replicon Cells of Hepatitis C Virus
... NS5A) and possibly host factors. These additional components may play important roles in modulating polymerase activity, conferring template specificity and ensuring RNA synthesis with necessary fidelity during the ...
6
Cell-Free Replication of the Hepatitis C Virus Subgenomic Replicon
... genomic replicon. The most frequent among these mutations were found to occur in NS3- and NS5A-coding sequences (6, 18, 24). A number of such tissue culture-adapted replicons acquire the ability to replicate at a ...
7
Characterization of Hepatitis C Virus Subgenomic Replicon Resistance to Cyclosporine In Vitro
... resultant replicon RNA for its ability to ...assay, replicon RNA carry- ing the P540A mutation had a significant reduction in the number of G418-resistant colonies formed in both ...of replicon RNA ...
12
Nonhepatic Cell Lines HeLa and 293 Support Efficient Replication of the Hepatitis C Virus Genotype 2a Subgenomic Replicon
... HCV replicon can replicate efficiently in two non-hepatocyte-derived cell ...replicating replicon RNA in HeLa and 293 cells were comparable to those in HepG2 ...JFH-1 replicon can replicate equally ...
5
Importance of Hydrogen Bond Contacts between the N Protein and RNA Genome of Vesicular Stomatitis Virus in Encapsidation and RNA Synthesis
... Dominant negative effect of R143A and R408A mutant N proteins. To further characterize the R143A and R408A mu- tant N proteins, we investigated whether these mutant proteins interfered with the ability of the wild-type N ...
11
gky061.pdf
... (HCV) subgenomic replicon, we found that the viral non- structural protein 5A (NS5A), a protein known to bind to viral RNA, also bound specifically to human cellu- lar transcripts that encode regulators of ...
11
Modulation of GB Virus B RNA Abundance by MicroRNA-122: Dependence on and Escape from MicroRNA-122 Restriction
... dicistronic subgenomic replicon RNA used in the present study. The replicon contains the GBV-B 5= UTR (nucleotides 1 to 459), which includes the GBV-B IRES, followed by the firefly luciferase ...the ...
10
Stearoyl Coenzyme A Desaturase 1 Is Associated with Hepatitis C Virus Replication Complex and Regulates Viral Replication
... (B) Subgenomic replicon cells derived from genotype 2a were treated with either DMSO or various concentrations of SCD1 ...and subgenomic replicon cells derived from either genotype 1b or 2a ...
15
Virion-Independent Transfer of Replication-Competent Hepatitis C Virus RNA between Permissive Cells
... because subgenomic replicon cells lacking viral structural genes were used in all experiments, and they appear to be dependent on an exosome-mediated, cell-cell transfer ...
6
Hepatitis C Virus Replication Is Modulated by the Interaction of Nonstructural Protein NS5B and Fatty Acid Synthase
... HCV subgenomic replicon cell line Sg-PC2 (19) was a gift from Jing-Hsiung Ou (University of Southern California, Los Angeles, ...HCV subgenomic replicon cell line Huh7/Rep-Feo (genotype 1b) ...
11
Participation of Rab5, an Early Endosome Protein, in Hepatitis C Virus RNA Replication Machinery
... Like most positive-strand RNA viruses, hepatitis C virus (HCV) is believed to replicate its genome on the surface of rearranged membranes. We have shown previously that HCV NS4AB, but not the product NS4B, inhibits ...
13
Novel Function of CD81 in Controlling Hepatitis C Virus Replication
... Plasmids. The HCV JFH1 full cDNA sequence cloned into plasmid pUC19 (pJFH-1) and the JFH1 subgenomic replicon plasmid (pSGR-JFH1/Luc) were described previously (46). Plasmid pJ6/JFH1-p7Rluc2A (20) was a ...
12
Selectable Subgenomic and Genome-Length Dicistronic RNAs Derived from an Infectious Molecular Clone of the HCV-N Strain of Hepatitis C Virus Replicate Efficiently in Cultured Huh7 Cells
... selectable subgenomic replicons derived from the Con1 strain of hepatitis C virus (HCV) are capable of autonomous replication in cultured Huh7 cells (Lohmann et ...a subgenomic replicon (NNeo/3-5B) ...
10
Involvement of Creatine Kinase B in Hepatitis C Virus Genome Replication through Interaction with the Viral NS4A Protein
... HCV subgenomic replicon and the DRM fractions in parental cells and then identified proteins that were more abundant in the fraction of HCV replicating ...HCV replicon, as determined using stable ...
11
Structure-function analysis of the equine hepacivirus 5′ untranslated region highlights the conservation of translational mechanisms across the hepaciviruses
... Equine hepacivirus (EHcV) (now also classified as hepacivirus A) is the closest genetic relative to hepatitis C virus (HCV) and is proposed to have diverged from HCV within the last 1000 years. The 5 ′ untranslated ...
14
Insights into the complexity and functionality of hepatitis C virus NS5A phosphorylation
... based subgenomic replicon (SGR-luc-JFH-1) or a full-length JFH-1 virus construct, allowing us to assess the role of phosphor- ylation at different stages of the virus life ...mutated subgenomic ...
13
Construction and Identification of Replicon of Contemporary Zika Virus Strain
... a subgenomic replicon of ZIKV GZ01, which was a vector containing Renilla luciferase (RLUC) reporter gene, and was Japanese encephalitis pAJE70 as ...GZ01 subgenomic replicon containing the ...
5