Super Resolution Microscopy
The synthesis and photophysical analysis of a series of 4-nitrobenzochalcogenadiazoles for super-resolution microscopy
... After solution phase analysis was complete, the blinking activity and hence suitability for super resolution microscopy was assessed by adopting a method recently described by Gibbs, et al. by ...
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Revealing t-tubules in striated muscle with new optical super-resolution microscopy techniques
... More recent advancements in t-tubule imaging have been achieved both with the newer optical super- resolution microscopy techniques and more recent EM volume imaging approaches. An example is ...
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Polysulfide-triggered fluorescent indicator suitable for super-resolution microscopy and application in imaging
... Illumination Microscopy (SIM). 10a,b Fluorescence microscopy has proved to be an invaluable tool for imaging studies as well as for studying the functions of specific analytes in living ...spatial ...
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Visualisation and analysis of hepatitis C virus non-structural proteins using super-resolution microscopy
... a super-resolution microscopy approach to visualise NS3 and NS5A in HCV infected ...localisation microscopy, both NS proteins were resolved as clusters of localisations smaller than the ...
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Between Life and Death: Reducing Phototoxicity in Super-Resolution Microscopy
... spatial resolution of an imaging system is defined as the capacity to distinguish closely separated features; in light mi- croscopy, this is limited by diffraction to ∼ 200 − 300 ...Consequently, microscopy ...
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Tracking HOCl concentrations across cellular organelles in real time using a super resolution microscopy probe
... optical microscopy has proven utility and ...fluorescence microscopy is spatial resolution. Even for high-resolution methods, such as confocal laser scanning microscopy (CLSM), two ...
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Repurposing a photosynthetic antenna protein as a super-resolution microscopy label
... Reconstruction Microscopy (STORM) and Structured Illumination Microscopy (SIM) have increased the achievable resolution of optical imaging, but few fluorescent proteins are suitable for ...
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Visualizing the replicating HSV 1 virus using STED super resolution microscopy
... Results: Using two colored probes labeling the same region of HSV-1 genome, the two probes highly correlated in both pre-replication and replicating genomes. In comparison, when probes from different regions were used, ...
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Injury-induced actin cytoskeleton reorganization in podocytes revealed by super-resolution microscopy
... glomerular super-resolution imaging using the Airyscan mode is the ability to directly view whole glomeruli stained with combinations of different ...
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Super-resolution microscopy reveals γ-secretase at both sides of the neuronal synapse
... The transmembrane protein assembly γ -secretase is a key protease in regulated intramembrane processing (RIP) of around 100 type-1 transmembrane proteins. Importantly, it has a pathological role in Alzheimer disease (AD) ...
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Polysulfide-triggered fluorescent indicator suitable for super-resolution microscopy and application in imaging
... Illumination Microscopy (SIM) images was carried out by using FIJI (ImageJ) ...Illumination Microscopy images obtained by using the Delta Vision OMX-SIM ...
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Tracking HOCl concentrations across cellular organelles in real time using a super resolution microscopy probe
... cells were first treated with 10 µM of SF-1 probe for 30 minutes and then treated with LPS (2500 ng) for 30 minutes and the LPS is removed from the cellular environment and replaced by growth media. The cells were then ...
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4D Super-Resolution Microscopy with Conventional Fluorophores and Single Wavelength Excitation in Optically Thick Cells and Tissues
... With this system, events from a sample containing both Alexa 647 and Alexa 750 fluorophores were generally detected in both channels. When the relationship between both channels was plotted, two clearly separated ...
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CellProfiler: Novel Automated Image Segmentation Procedure for Super-Resolution Microscopy
... Super resolution (SR) microscopy unlocked new oppor- tunities for cell biologists to investigate cells and cellular functions at unprecedented resolution up to few nano- meters, which require ...
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The use of nanoscale fluorescence microscopic to decipher cell wall modifications during fungal penetration
... as super-resolution microscopy (Hell, 2007; Agrawal et ...(STED) microscopy with a possible resolution of 35 nm in the far field (Hell and Wichmann, 1994), but without reports of ...
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Monitoring HIV-1 Assembly in Living Cells: Insight from Dynamic and Single Molecule Microscopy
... optical microscopy, combining high speed and high spatial resolution now permit to study assemblies and their consequences at the single molecule level within (living) ...advanced super ...
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Quantitative super-resolution imaging of Bruchpilot distinguishes active zone states
... of super-resolution microscopy is the development of analytical tools to quantify data sets and to help provide biological interpretations 30 ...(STED microscopy displays B9 ‘dots’ per AZ 41 ...
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Drosophila Oocytes as a Model for Understanding Meiosis: An Educational Primer to Accompany “Corolla Is a Novel Protein That Contributes to the Architecture of the Synaptonemal Complex of Drosophila”
... used super-resolution microscopy techniques to localize the protein within the SC and a classic molecular assay (yeast two- hybrid) to identify protein interactions between the new protein and other ...
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Super-Resolution and Iterative Cascaded De- blocking of Compressed Images
... Here we consider a reference patch within a low resolution compressed image After that patch clustering is done to find out its similar patches within a given window. Euclidean distance is considered as a ...
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A SURVEY ON QUALITY FACE EXTRACTION FROM VIDEO
... need human operators to identify suspected people. Therefore, having an automated system working with LR and low-quality face images is desirable. However, low-quality images do not have enough high-resolution ...
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