Surface-enhanced laser desorption/ionization
Use of Surface-Enhanced Laser Desorption/Ionization with Time of Flight (SELDI-TOF) of the Urine in the Assessment of Acute Kidney Injury (AKI)
... Surface-enhanced laser desorption/ionization-time of flight (SELDI-TOF) mass spectrometry was used to generate protein profile spectra from the urine ...array surface for ...
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Optimization and evaluation of surface-enhanced laser-desorption/ionization time-of-flight mass spectrometry for protein profiling of cerebrospinal fluid
... ProteinChip surface- enhanced laser-desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) is one such approach which offers a unique platform for high throughput ...
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Optimising the Use of TRIzol-extracted Proteins in Surface Enhanced Laser Desorption/ Ionization (SELDI) Analysis
... Results: To facilitate the use of TRIzol-extracted proteins, we first compared the ability of four different common solubilizing reagents to solubilize the TRIzol-extracted proteins from an osteosarcoma cell line, U2-OS. ...
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Assessment of protein stability in cerebrospinal fluid using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry protein profiling
... At 4°C, the peaks between 13.7 and 14.1 kDa highlighted by “a” at 0 h resolve to a pro- tein doublet (*) by 8–12 h at 4°C (Fig. 2, left panels). Finally, the spectrum observed after 6 h at 23°C is almost identical to the ...
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Interest of major serum protein removal for Surface-Enhanced Laser Desorption/Ionization – Time Of Flight (SELDI-TOF) proteomic blood profiling
... teomics approaches and they mask or interfere with the detection of the other low amount protein components. This situation explains why the discovery of new proteins or peptides biomarkers in blood is challenging. To ...
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Proteomics profiling of urine with surface enhanced laser desorption/ionization time of flight mass spectrometry
... For the seven chip types tested, chip protocols used were based on standard protocols provided by the chip manu- facturer Ciphergen Biosystems. The sample was applied manually to eight spots per condition (seven chip ...
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The discovery and identification of a candidate proteomic biomarker of active tuberculosis
... possible. Surface- enhanced laser desorption ionization time of flight mass spectrometry (SELDI-TOF MS), as a powerful proteo- mics technology integrating the technologies of chips/ ...
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Biomarkers of inflammation and innate immunity in atrophic nonunion fracture
... pathology. Surface- enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-TOF–MS) and two-dimensional difference gel electrophoresis (2D-DIGE) techniques are both ...
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The new platinum-based anticancer agent LA-12 induces retinol binding protein 4 in vivo
... the surface- enhanced laser desorption-ionization time-of-flight mass spectrometry (SELDI-TOF MS) approach [18] we identi- fied Retinol-binding protein 4 (RBP4) as significantly ...
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Proteomic biomarkers predicting lymph node involvement in serum of cervical cancer patients. Limitations of SELDI-TOF MS
... SELDI: Surface-enhanced laser desorption; TOF: Time of flight; MS: Mass spectrometry; MARS: Multiple affinity removal system; HPLC: High pressure liquid chromatography; CM10: Weak cation ...
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Comparative proteomic analysis of normal and tumor stromal cells by tissue on chip based mass spectrometry (toc-MS)
... the laser-based tissue microdissection ...assisted laser desorption and ionization) seems to be a better choice as shown in sev- eral studies using microdissected tissue ...(surface ...
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Optimization of Matrix Enhanced-Surface Assisted Laser Desorption Ionization Mass Spectrometry Imaging (ME_SALDI MSI) for Preliminary Studies Involving Supported Lipid Bilayers.
... label-free surface characterization technique, is a powerful tool that provides the identity and spatial locale of molecules within a ...soft ionization method, matrix assisted laser ...
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Surface-Assisted Laser Desorption/Ionization-Mass Spectrometry (SALDI-MS) of Controlled Nanofeatures and the Associated Thermal Properties
... that ionization is based on aqueous phase basicities instead of gas phase ...the ionization was increased by the presence of ...and desorption of ions from a thin water layer on a field emitter tip ...
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Utility of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry following Introduction for Routine Laboratory Bacterial Identification
... Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) was evaluated prospectively in a diagnostic laboratory. Nine hundred twenty-seven organisms were tested in ...
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Comparison of Two Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry Methods with Conventional Phenotypic Identification for Routine Identification of Bacteria to the Species Level
... outset. Enhanced strategies include the use of multiplexing (14, 26) or of highly parallel techniques, such as diagnostic DNA microarrays (5, 8), to amplify and detect multiple sequences at ...
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Impact of matrix assisted laser desorption/ionization time of flight mass spectrometric evaluation on the clinical outcomes of patients with bacteremia and fungemia in clinical settings lacking an antimicrobial stewardship program: a pre post quasi experimental study
... matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) combined with antimicrobial stewardship in patients with positive blood cultures; clinical outcomes ...
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Matrix-Assisted Laser Desorption Electrospray Ionization: Fundamental Principles and Applications Toward Direct Analysis and Molecular Imaging.
... The laser power was attenuated using an external attenuator to values of 210 μJ/pulse - ...The laser beam was directed using two gold coated Pyrex mirrors (Newport Corporation, Irvine, CA, USA) and was ...
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Discrepancy in MALDI-TOF MS identification of uncommon Gram-negative bacteria from lower respiratory secretions in patients with cystic fibrosis
... MALDI-TOF MS measurements were performed with a 60-Hz nitrogen laser and the spectra were analyzed over a mass range of 2–20 kDa. The strains from both reference and clinical sets were measured and identified by ...
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Anti-biofilm and antiradical activity of different essential oils
... Figures 7-9 presents the dendrogram generated by the first MALDI-TOF MS Biotyper experiment. These results indicated that the MSPs of the groups were distinguishable by the use of MALDI profiling approach, since they ...
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The Genus Corynebacterium and Other Medically Relevant Coryneform Like Bacteria
... assisted laser desorption ionization–time of flight (MALDI-TOF) analysis is rapidly becoming a routinely used tool in many micro- biology laboratories, whereby proteins liberated from bacteria are ...
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