Target genes from microarray validated by qPCR
Improving RNAi in the Brown Marmorated Stink Bug: Identification of Target Genes and Reference Genes for RT-qPCR
... In addition, 23 original papers have reported on the latest research on biology and management of BMSB 6 . After the discovery of RNA interference (RNAi) in the nematode, Caenorhabditis elegans and demonstration of its ...
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Cowpea and abiotic stresses: identification of reference genes for transcriptional profiling by qPCR
... the target genes, both qPCR and HT-SuperSAGE approaches presented a 56% agreement on ...The target genes rep- resent promising candidates for biotechnological use after these ...
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Reference Genes for RT qPCR Analysis of Environmentally and Developmentally Regulated Gene Expression in Alfalfa
... Tub genes led to strikingly different conclusions including a lack of a low temperature effect on SuSy expression, a much higher increase in SPS gene expression and unexpected cold inducibility of ...reference ...
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Permutation validated principal components analysis of microarray data
... selected genes reflecting the contrast between the groups w1, w7 on one side and s7 and k0 on the other side because this contrast was captured by the first two ...of genes should be treated with caution ...
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Postmenopausal Osteoporosis reference genes for qPCR expression assays
... reference genes (RG) should be overcome to ensure the quality and reproducibility of gene expression ...stable genes in OP studies while ACTB, 18 S and HPRT1 were inadequate for normalization in our data ...
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Analysis of Differential Expression of microRNAs and Their Target Genes in Prostate Cancer: A Bioinformatics Study on Microarray Gene Expression Data
... their target genes at post- transcriptional or translational levels ...multiple genes (34, ...high-throughput microarray datasets to investigate changes in the expression of microRNAs in 99 ...
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Selection of reference genes for qPCR in hairy root cultures of peanut
... evaluated genes had more variation after NaOAc than MeJA ...the genes with low expression ...reference genes for normalization was calcu- lated to be two genes (TBP2 and RPL8C) using geNorm ...
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Triple-target microarray experiments: a novel experimental strategy
... for genes should be identical across array dye-label ...outcomes from standard dual-target ...obtained from the three individual samples on an array ...away from zero ...
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Deconvolution of Chromatin Immunoprecipitation-Microarray (ChIP-chip) Analysis of MBF Occupancies Reveals the Temporal Recruitment of Rep2 at the MBF Target Genes
... pensable component (7). To identify the genomic binding sites for the MBF complex, we first determined the binding sites for Cdc10 in cells bearing a sole chromosomal copy of the cdc10-HA allele by the use of the ...
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Microarray based transcriptomics and the search for biomarker genes in zebrafish
... treated from 24- 48 hpf with 10 different ...pressed genes. I validated my findings using literature ...biomarker genes, I grouped the compounds based on the identified modes of action and ...
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Discovering monotonic stemness marker genes from time-series stem cell microarray data
... marker genes such as OCT4, NANOG, BLBP, discovered from the ESCN dataset exhibit consistent behavior with that reported in other ...monotonic genes found by MFSelector in either stemness or ...
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Production of DNA microarray and expression analysis of genes from Xylella fastidiosa in different culture media
... plant genes (707050B11-Rubisco), as well as synthetic genes from several species (human, mouse, rat, yeast, plant and bacteria) were also included in this array as negative ...in microarray ...
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RNA microarray analysis in prenatal mouse cochlea reveals novel IGF-I target genes: implication of MEF2 and FOXM1 transcription factors
... cochleae from Igf1 2 / 2 mice there is increased neuronal apoptosis and delayed neuronal maturation, but there is no evidence for altered cellular proliferation or cell damage ...expressed genes in the ...
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RNA microarray analysis in prenatal mouse cochlea reveals novel IGF-I target genes: implication of MEF2 and FOXM1 transcription factors
... cochleae from Igf1 2/2 mice there is increased neuronal apoptosis and delayed neuronal maturation, but there is no evidence for altered cellular proliferation or cell damage ...expressed genes in the ...
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Identification of new reference genes for the normalisation of canine osteoarthritic joint tissue transcripts from microarray data
... [22]. Microarray data analysis indicated the mitochondrial ribosomal protein S25 was also stably expressed, although it was only stably expressed in two of the four tissues analysed by RT-qPCR (cartilage ...
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Density Based Pruning for Identification of Differentially Expressed Genes from Microarray Data
... algorithms from the 17 datasets with or with- out DB ...DEGs from the 17 datasets when K=150 predictions are allowed for each ...550 genes are allowed to predict for each ...benefit from DB ...
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Selection of reference genes from Shiraia bambusicola for RT-qPCR analysis under different culturing conditions
... new genes, SbLAlv9, SbJsn1 and SbSAS1, indicated appropri- ate properties for all the nutrient and light ...reference genes could indeed provide the more reliable genes and improve the assurance of ...
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A weighted average difference method for detecting differentially expressed genes from microarray data
... tal datasets is more important than a profound one on a few experimental datasets when estimating the methods' practical ability to detect DEGs, as the superficial compar- ison on a large number of datasets can also ...
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The effect of target secondary structure on microarray data quality
... diagnostic microarray for the three pathogenic species of genus ...the target molecules, we decided to conduct a secondary structure modeling simulation using a genome wide set of Brucella suis 1330 open ...
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Listen to genes : dealing with microarray data in the frequency domain
... temporal microarray data ...; from processing of raw data to gene network ...the genes according to their functionality but also allows a purely data driven natural ranking of genes based on ...
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