TCR-T
Homeostasis and function of regulatory T cells (Tregs) in vivo:lessons from TCR-transgenic Tregs
... BDC2.5 TCR. Using TCR-based lineage tracing, the authors showed that conversion from the conventional to the regulatory T-cell compartment occurred only at very low frequencies both within the ...
17
TCR-triggered extracellular superoxide production is not required for T-cell activation
... in T-cell specific super- oxide production occurred 10 min after TCR-triggering and ROS levels decreased gradually ...human T-cell blasts and Jurkat T cells (data not ...human T cells ...
10
Nondeletional T-Cell Receptor Transgenic Mice: Model for the CD4+ T-Cell Repertoire in Chronic Hepatitis B Virus Infection
... 11/4-12 TCR-Tg mice into both HBeAg-Tg and HBcAg-Tg recipients resulted in relatively mild relapsing and remitting chronic liver injury, whereas adoptive transfer of Th1 cells cultured in vitro with HBeAg from ...
13
Antigen-Driven T-Cell Selection in Patients with Cervical Cancer as Evidenced by T-Cell Receptor Analysis and Recognition of Autologous Tumor
... sorted T cells (using immunomagnetic beads) was carried out as described in detail previously ...29 TCR VA- and 24 VB-specific primer pairs, and a runoff reaction using a fluorophore-labeled TCR-CA- ...
12
TCR-clonotyping-based Analysis of a Frenemy —Public T Cells
... mature T-cell receptor (TCR) specially recognizes a peptide presented by self MHC molecule, adaptive immune responses are selectively ...diverse TCR repertoire during a lifelong time [1, ...a ...
6
Improved Assessment of T-Cell Receptor (TCR) VB Repertoire in Clinical Specimens: Combination of TCR-CDR3 Spectratyping with Flow Cytometry-Based TCR VB Frequency Analysis
... variable TCR alpha chain and with 24 individual primers specific for the TCR beta chain as described previously (8, ...fluorophore-labeled TCR C-alpha chain-specific primer (5⬘-AT ...
10
Effective NY ESO 1–specific MHC II–restricted T cell receptors from antigen negative hosts enhance tumor regression
... of T cell receptor–engineered (TCR- engineered) tumor-reactive T cells is a promising approach to treat solid ...therapeutic TCR to its cognate peptide-MHC mol- ecule on the ...during T ...
13
The Role of Slp-76 Phosphotyrosines in TCR Signal Transduction and T Cell Differentiation
... the TCR (Lin and Weiss, ...the TCR signaling cascade, how it is regulated remains largely ...of TCR triggering – how ligation of the extracellular domain of the TCR complex results in the ...
141
Conferring indirect allospecificity on CD4+CD25+ Tregs by TCR gene transfer favors transplantation tolerance in mice
... the TCR-transduced Tregs was confirmed in vitro. In mice, TCR- transduced Tregs, but not dTregs, induced long-term survival of partially MHC-mismatched heart grafts when combined with short-term adjunctive ...
11
T cell receptor-engineered T cells for leukemia immunotherapy
... of TCR genes into primary human T cells to endow their specificity toward leukemia cells is becoming an interesting tool for T cell immunotherapy for hemato- logical ...of TCR-T cells ...
7
TCR activation kinetics and feedback regulation in primary human T cells
... the TCR are split to activate different effector ...the TCR and discriminates between negatively and positively selecting ligands ...mature T cells directing signals emanating from the TCR ...
11
Generation of diffuse large B cell lymphoma-associated antigen-specific Vα6/Vβ13+T cells by TCR gene transfer
... of TCR gene transduction. In human subjects, normal autologous T lymphocytes, transduced ex vivo with anti- tumor associated antigen (TAA) and the TCR genes, which were re-infused into cancer ...
9
Chronic TCR-MHC (self)-interactions limit the functional potential of TCR affinity-increased CD8 T lymphocytes
... defined TCR variant similar between A2 pos and A2 neg T cells, indicating that the sensitivity of TCR triggering for a given TCR affinity variant was fully preserved (Additional file 1: Figure ...
17
Proton magnetic resonance spectroscopy assessment of metabolite status of the anterior cingulate cortex in chronic pain patients and healthy controls
... Glu/ tCr (p=0.039) and Glx/tCr (p<0.001) and lower levels of NAA/tCr than controls, although this did not reach statistical significance (p = ...
7
Elongated TCR alpha chain CDR3 favors an altered CD4 cytokine profile
... CD4 T lymphocyte activation requires T cell receptor (TCR) engagement by peptide/MHC (major histocompatibility complex) ...The TCR complementarity-determining region 3 (CDR3) contains variable ...
24
T-Cell Receptor (TCR) Clonotype-Specific Differences in Inhibitory Activity of HIV-1 Cytotoxic T-Cell Clones Is Not Mediated by TCR Alone
... promoter. TCR ␣ and  genes were linked by a self-cleaving P2A peptide followed by an IRES-driven GFP marker gene to allow visualization of the transduced cells by flow ...KK10-specific TCR were determined by ...
18
A CD22-reactive TCR from the T-cell allorepertoire for the treatment of acute lymphoblastic leukemia by TCR gene transfer
... Isolated T-cell clones were analyzed for binding to specific pMHC- tetramers and CD8 expression by staining with PE-labelled pMHC-tetramers, and an Alexa700-conjugated antibody against CD8 (Invitrogen/Calteg) ...
12
Co receptor CD8 mediated modulation of T cell receptor functional sensitivity and epitope recognition degeneracy
... between T-cell receptors (TCRs) and peptide epitopes is highly degenerate: a TCR is capable of interacting productively with a wide range of different peptide ligands, involving not only cross-reactivity ...
12
TCR-ligand dissociation rate is a robust and stable biomarker of CD8+ T cell potency
... the TCR-pMHC interactions (e.g., K D , k off , k on ) could better predict T cell activation and subsequent response ...of T cell responsiveness (44, ...the TCR-pMHC binding ...same ...
19
T-cell receptor-optimized peptide skewing of the T-cell repertoire can enhance antigen targeting
... of T-cells” ...of T-cells that bear TCRs with poor reactivity against the natural antigen ...A-specific T-cell population is characterized by a highly diverse TCR repertoire and, as a result, ...
21