temperature-sensitive simian virus 40
Temperature-Sensitive Simian Virus 40-Transformed Cells: Phenomena Accompanying Transition from the Transformed to the “Normal” State
... It may be important to note that: i the shedding of dead cells ends at a point in which the cultures have reached the density characteristic of their saturation at 39 C, and the cells re[r] ...
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Temperature-Sensitive Simian Virus 40 Mutant Defective in a Late Function
... To determine whether mutant SV40tsTNG-1 also induced cellular DNA synthesis, 5-day-old cultures of primary mouse kidney cells 3.3 X 106 cells per culture were infected at input multiplic[r] ...
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Analysis of simian virus 40 wild-type and mutant virions by agarose gel electrophoresis.
... The virions of a temperature-sensitive strain of simian virus 40, tsB204, have significantly greater mobility than those of wild-type simian virus 40, when electrophoresis was conducted [r] ...
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T-antigen expression in proliferating and non-proliferating simian virus 40-transformed mouse cells.
... Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Previous studies with simian virus 40-transformed mouse 3T3 cells which are temperature sensitive for the expression of [r] ...
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Biological and biochemical studies of cells transformed by simian virus 40 temperature-sensitive gene A mutants and A mutant revertants.
... Virus recovered from both of the tsA30-transformed cell lines was temperature sensitive for viral DNA synthesis in BSC-1 cells, as is characteristic of SV40 A mutants Table 2.. That reco[r] ...
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Temperature-sensitive BC mutants of simian virus 40: block in virion assembly and accumulation of capsid-chromatin complexes.
... In addition, we have tested the state of the VP1 protein produced in cells infected with selected mutants of the BC group tsBC216, tsBC223, or tsB248 and found that a stable form of this[r] ...
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Separation of lytic and transforming functions of the simian virus 40 A region: two mutants which are temperature sensitive for lytic functions have opposite effects on transformation.
... 518-528 0022-538X/81/050518-11$02.00/0 Separation of Lytic and Transforming Functions of the Simian Virus 40 A Region: Two Mutants Which Are Temperature Sensitive for Lytic Functions Hav[r] ...
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Oligomerization of simian virus 40 large T antigen is not necessarily repressed by temperature-sensitive A gene lesions.
... tsA1499-infected cells were kept for 96 h at 32 or 41°C, radiolabeled with [32P]phosphate, extracted, and analyzed for both T-p53 complexes and free T antigen by sequential immunoprecipi[r] ...
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Temperature-sensitive mutants identify crucial structural regions of simian virus 40 large T antigen.
... SCHWEDES,' AND PETER TEGTMEYER1* Department of Microbiology, State University of New York, Stony Brook, New York 11794-8621,' and Department of Microbiology, Specialized Cancer Research [r] ...
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Recombination Between Endogenous and Exogenous Simian Virus 40 Genes. I. Rescue of a Simian Virus 40 Temperature-Sensitive Mutant by Passage in Permissive Transformed Monkey Lines
... Rescue of a Simian Virus 40 Temperature-Sensitive Mutant by Passage in Permissive Transformed Monkey Lines Received for publication 11 May 1977 Passage of the simian virus 40 SV40 temper[r] ...
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DNA infectivity and the induction of host DNA synthesis with temperature-sensitive mutants of simian virus 40.
... This, and the fact that mutants of the A group induce host DNA synthesis in CV-1 cells although they do not replicate viral DNA at 40 C 5, 18 suggested either that an early function othe[r] ...
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Isolation and characterization of a heat-inducible simian virus 40 mutant.
... 374 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest We have isolated a new type of temperature-sensitive mutant of simian virus 40 SV40 that is capable of productive in[r] ...
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Viral DNA Synthesis in Cells Infected by Temperature-Sensitive Mutants of Simian Virus 40
... For the initial screening of mutants data in Table 1 the 24 well plates of Linbro Chemical Co., New 116 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Temperature-sens[r] ...
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Simian virus 40 mutants carrying two temperature-sensitive mutations.
... Double mutants of A:B, A:C, and A:BC complementation groups, like their A parent, were defective in viral DNA replication, in the induction of host DNA synthesis, and in the transformati[r] ...
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Characterization of T antigen in cells infected with a temperature-sensitive mutant of simian virus 40.
... The present study demonstrates that a molecular form of T antigen induced by ts 900, a transformation-defective ts mutant of SV40 29, is more heat labile than that induced by WT virus, i[r] ...
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Temperature-Sensitive Mutants of Simian Virus 40: Infection of Permissive Cells
... Plaque characteristics of wild-type and mutant simian virus 40 SV40 stocks propagated at 32 C Virus Efficiency of plating39 C/32 C Time of plaque appearance in days d Infected cultures w[r] ...
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DNA Binding Properties of Simian Virus 40 Temperature-Sensitive A Proteins
... The binding regions were defined by the ability of A protein to protect simian virus 40 DNA from digestion with limited footprint assay or excess fragment assay amounts of DNase I.. At l[r] ...
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Characterization of simian cells tranformed by temperature-sensitive mutants of simian virus 40.
... In addition, the role of the gene A product if any in the maintenance of the transformed 1106 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Seven lines derived from p[r] ...
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Temperature-sensitive mutants of simian virus 40 selected by transforming ability.
... Therefore, to analyze further the mechanism of transformation, we have tried to isolate mutants of SV40 i which are temperature sensitive in their ability to transform nonpermissive rat [r] ...
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Products of complementation between temperature-sensitive mutants of simian virus 40.
... These results imply that i the D product may be synthesized late in infection; ii therefore, any defect exhibited by D virions early in infection probably results from the introduction o[r] ...
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