Transient receptor potential vanilloid 2
Reduced transient receptor potential vanilloid 2 expression in alveolar macrophages causes COPD in mice through impaired phagocytic activity
... C57BL/6 mice (weight between 16.5 and 20.7 g) by ex- posure to the smoke of four unfiltered cigarettes per day (3R4F, University of Kentucky), 6 days per week for 2 and 6 months, by using an experimental-smoking ...
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Post-transcriptional regulation of 5’-untranslated regions of human Transient Receptor Potential Vanilloid type-1 (TRPV-1) channels: role in the survival of glioma patients
... Total RNA (RNAt), from cell lines, was extracted using the RNeasy Mini kit (Qiagen, Milan, IT). RNAt from GBM biopsies was extracted with RNeasy FFPE kit (Qiagen, Milan, IT), as previously described [44]. Total RNA from ...
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Implication of Transient Receptor Potential Vanilloid Type 1 in 14,15-Epoxyeicosatrienoic Acid-induced Angiogenesis
... contained 2% fetal bovine serum (FBS) (GibcoBRL, Grand Island, NY, USA), 1 µg/ml hydrocortisone, 10 ng/ml human epidermal growth factor, 3 ng/ml human fibroblast growth fac- tor, 10 µg/ml heparin, and 1% ...
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Capsazepine, a Transient Receptor Potential Vanilloid Type 1 (TRPV1) Antagonist, Attenuates Antinociceptive Effect of CB1 Receptor agonist, WIN55,212-2, in the Rat Nucleus Cuneiformis
... Several lines of evidence indicate the existence of a cannabinoid pain modulatory system in different brain regions including the PAG (Hohmann et al., 2005; Mar- tin et al., 1999; Palazzo et al., 2001; Suplita, Richard, ...
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Transient Receptor Potential Vanilloid 1 Immunoreactive Innervation Increases in Fractured Rat Femur
... week, 2 weeks, and 4 weeks ...and 2 weeks and 4 weeks after frac- ture ...TRPV1, transient receptor potential vanilloid 1; DRG, dorsal root ganglion; ir, ...
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Expression of transient receptor potential vanilloid (TRPV) channels in different passages of articular chondrocytes
... previously [17,41,42]. Cartilage shavings were digested with a freshly prepared solution of 0.1% type I collagenase in free serum DMEM for 18 h at 37 °C. At the end of the digestion period any remaining undigested ...
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Transient receptor potential (TRP) gene superfamily encoding cation channels
... Group 2 TRPs is based on the human phenotypes that the founding genes cause when mutated — polycystic kinase disease (PKD) and mucolipidosis type IV (MCOLN, Mucolipin) — while their encoded proteins have also been ...
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Lack of transient receptor potential vanilloid 1 channel modulates the development of neurogenic bladder dysfunction induced by cross-sensitization in afferent pathways
... Mice were anesthetized with 2% isoflurane and held on a warming pad inside the designated hood to minimize an investigator`s exposure to the anesthetic. A midline laparotomy was performed under the sterile ...
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Paeoniflorin protects diabetic mice against myocardial ischemic injury via the transient receptor potential vanilloid 1/calcitonin gene-related peptide pathway
... Fig. 2 TRPV1 gene knockout partially abrogates paeoniflorin (PF)-mediated cardioprotection against MI. a TRPV1 knockout attenuated the decrease in myocardial infarct size by PF treatment, the black arrow indicates ...
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Implication of AMP-Activated Protein Kinase in Transient Receptor Potential Vanilloid Type 1-Mediated Activation of Endothelial Nitric Oxide Synthase
... The investigation conformed to the Guide for the Care and Use of Laboratory Animals published by the US National Institutes of Health (23), and all animal experiments were approved by the Ani- mal Care and Utilization ...
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Forsythoside A exerts antipyretic effect on yeast-induced pyrexia mice via inhibiting transient receptor potential vanilloid 1 function
... Transient receptor potential vanilloid 1 (TRPV1) is a non-selective cation channel gated by noxious heat, playing major roles in ...E 2 (PGE 2 ) and interleukin ...
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Original Article Upregulation of the transient receptor potential vanilloid 1 in colonic epithelium of patients with active inflammatory bowel disease
... From all IBD patients, endoscopic biopsies were taken from both affected and non-affect- ed colonic mucosa using standard biopsy for- ceps. For histological analyses, at least three biopsies were collected, fixed in 10% ...
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THE ROLE OF TRANSIENT RECEPTOR POTENTIAL VANILLOID 1 RECEPTOR IN DESIPRAMINE INDUCED ANALGESIC EFFECT IN DIABETIC MICE
... 2. Radiant heat tail-flick method: Tail-flick latency was assessed by the analgesiometer (Instruments manufacturing corporation -model/MC-102413, India). The strength of the current passing through the naked ...
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Transient receptor potential Vanilloid 1 (TRPV1) in haematological malignancies
... Expression of TRPV1 in leukocytes obtained from patients with a range of haematological malignancies, including multiple myeloma (MM) and B-cell non- Hodgkin’s Lymphoma (B-NHL), was then investigated (Chapter 6). TRPV1 ...
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A Novel Mechanism for Calmodulin Dependent Inactivation of Transient Receptor Potential Vanilloid 6
... the vanilloid subfamily of the Transient Receptor Potential (TRP) superfamily of ion channels and both play an important role in overall Ca 2+ ...Ca 2+ Adependent feedback ...
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Transient receptor potential Vanilloid 1 (TRPV1) in haematological malignancies
... 1999). Vanilloid-like agents may affect cellular function through two mechanisms; 1) the interaction with the TRPV1 receptor, or 2) direct interaction with the cell due to their ability to cross the ...
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Preventive Effect of SA13353, a Novel Transient Receptor Potential Vanilloid 1 Agonist, on Ischemia/Reperfusion Induced Renal Injury in Rats
... Male Sprague-Dawley rats (10 weeks of age; Japan SLC) weighing 280 to 320 g were used. The animals were housed in a light-controlled room with a 12-h light/dark cycle and were allowed ad libitum access to food and water. ...
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Interaction Between the Cannabinoid and Vanilloid Systems on Anxiety in Male Rats
... coupled receptor found in the mammalian brain (Matsuda, Lolait, Brownstein, Young, & Bonner, 1990) and are particularly abundant in the cortex, hippocampus, nucleus accumbens, and amygdala (Millan, ...
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Mechanism and therapeutic effectiveness of nerve growth factor in osteoarthritis pain
... its receptors is an issue of debate. Recently, the development of surface plasmon resonance technology has enabled evalu- ation of the inhibitory potential of ALE-0540, PD90780, Ro 08-2750, and PQC083. These ...
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OConor_unc_0153D_14695.pdf
... Transient Receptor Potential (TRP) cation channels serve as polymodal sensors in mammalian cells and come in six subtypes: TRPC (“canonical”), TRPV (“vanilloid”), TRPM (melastatin”), TRPP ...
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