Two-photon laser scanning microscopy
Reduction of the pulse duration of the ultrafast laser pulses of the Two Photon Laser Scanning Microscopy (2PLSM)
... Glan Laser polarizer GL10 from THORLABS (see ...Glan Laser Calcite Polarizer is manufactured from select portions of the calcite crystal, making it an appropriate choice for high energy laser ...
7
Super resolution Imaging by Two photon Structured Illumination Microscopy
... of two-photon excitation to confocal laser scanning microscope (CLSM) leads two-photon laser scanning microscopy (TPLSM) to become an important tool in ...of ...
7
Two-photon laser scanning fluorescence microscopy using photonic crystal fibre
... Multi-photon laser scanning microscopy (MPLSM) is a well-established tool for deep and live imaging of biological tissue 1 .... Two types of laser source suited to ...
18
Widefield two-photon excitation without scanning : live cell microscopy with high time resolution and low photo-bleaching
... the laser intensity was increased ...faster scanning mirrors have been used [4], and parallelism has been achieved in two-photon imaging by the use of slit scanning [5] or the ...
19
Methanol immersion reduces spherical aberration of water dipping lenses at long wavelengths used in multi-photon laser scanning microscopy
... a two-fold increase in fluorescence signal intensity using adaptive optics when imaging a mouse gut similar to the specimen used in this paper [27] although, enhancements of up to five-fold have also been observed ...
12
An update : improvements in imaging perfluorocarbon mounted plant leaves with implications for studies of plant pathology, physiology, development and cell biology
... of microscopy: laser scanning confocal microscopy (LSCM), two-photon fluorescence microscopy, second harmonic generation microscopy, and stimulated Raman scattering ...
9
Towards Heralded Two-Photon Absorption and Two-Photon Excited Fluorescence for Quantum Microscopy and Spectroscopy
... fluorescence photon is emitted subsequently to the absorption process (see ...derived two-photon absorption theoretically in her thesis in 1931 using quantum theory and the Dirac notation ...and ...
16
Dual-modality optical diagnosis for precise in vivo identification of tumors in neurosurgery
... fiber scanning probe to obtain the swept source OCT (SS-OCT) images and the fluorescence spectrum ...OCT scanning line. However, the two kinds of information were not combined to provide diagnostic ...
16
Bioluminescence imaging and two-photon microscopy guided laser ablation of GBM decreases tumor burden
... intensity. Laser resection included the two ...Nd:YAG laser source with a wavelength of 1,064 nm (HGL-MY100C, Wuhan Huagong Laser Engineering, China) in the laser ablation ...The ...
14
Fluorescence excitation analysis by two-photon confocal laser scanning microscopy: a new method to identify fluorescent nanoparticles on histological tissue sections
... of fluorescence intensity of each pixel in the spectral emission image, or excitation (Figure 1). The analysis assumes that each pixel is a mixture of fluorescence patterns, and the aim is to unmix those patterns. ...
10
Heparin binding protein targeted to mitochondrial compartments protects endothelial cells from apoptosis
... Neutrophil-borne heparin-binding protein (HBP) is a multifunctional protein involved in the pro- gression of inflammation. HBP is stored in neutrophil granules and released upon stimulation of the cells in proximity to ...
11
Lipoprotein (a) Internalization by RAW 264.7 Cells is Associated with Morphological Changes and Accumulation of Lipids Detectable by Two-photon Scanning Microscopy
... and Nile Red are capable of a greater solubility in lipids than in water and are therefore well suited to identify lipids. While the former can be observed in direct light, the latter is almost non- fluorescent in water ...
12
Thiolate self assembled monolayers studied with a tuneable infrared low temperature laser driven scanning tunnelling microscope
... into two groups; those that are recorded over the cluster and those that are ...these two scenarios so that they could be compared to see what difference (if any) could be ...
232
Electron microscopic measurement of the size of the optical focus in laser scanning microscopy
... The laser source for nanoablation was a free-space-coupled Ti:Sapphire laser ~Chameleon, Coherent ...The laser beam was deflected by galvanometer scanning mirrors and then via a scan lens into ...
8
Drastic effect of shearing on graphite microtexture: attention and application to Earth science
... XR laser Raman spectroscope (Thermo Fisher Scientific) at the Petrology and Mineralogy La- boratory at Nagoya University, Nagoya, ...Nd-YAG laser passed through a confocal microscope (BX51; Olympus, Tokyo, ...
12
<p>Nano-Scaled Zeolitic Imidazole Framework-8 as an Efficient Carrier for the Intracellular Delivery of RNase A in Cancer Treatment</p>
... confocal laser scanning microscopy (CLSM), and the inhibition of cell proliferation after the delivery of RNase A was evaluated by MTT assay, Live/Dead staining, and TUNEL cell apoptosis analysis, ...
11
Confusion over live/dead stainings for the detection of vital microorganisms in oral biofilms - which stain is suitable?
... At first glance the same holds true for the incubation procedure (line (d) in Table 3), however, this might be a more severe problem. Twenty-two of the users did not state the procedure or relied on the ...
12
<p>Comparative Two-Photon Fluorescence Microscopy Analysis of Riboflavin Penetration in Two Different Solutions: Dextran and Hydroxypropyl Methylcellulose</p>
... Figure 4 shows ribo fl avin concentration in the cornea obtained from multiphoton microscopy. The maximum scale of the concentration graph was standardized as 0.1% because the ribo fl avin solutions had the same ...
8
The study of n-3PUFAs protecting the intestinal barrier in rat HS/R model
... associated with the change of fatty acids in plasma and epithelial cells (Tables 3 and 4). It is the administration of ω-3 PUFAs that promotes the formation of eicosanoids, which are anti-inflammatory rather than ...
9
Multifunctional scanning ion conductance microscopy
... for Scanning Electrochemical Probe Microscopy: While the above techniques have successfully been used to study cellular uptake on a supercellular scale, very few are capable of the investigation of single ...
165