Why collect fluorescence from single molecules?
Quantifying fluorescence enhancement for slowly diffusing single molecules in plasmonic near fields
... calculated from the unbinned time trace to exhibit the characteristic time of a process of interest, such as the diffusion 22,23 or rota- tion 24 characteristic times of molecules in solution as well as ...
11
Structure and Mechanics of Proteins from Single Molecules to Cells
... Proteins from Single Molecules to Cells Abstract Physical factors drive evolution and play important roles in motility and attachment as well as in ...used single molecule force spectroscopy ...
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Laser-influenced fluorescence spectroscopy of rubidium atoms and mercury molecules.
... the fluorescence also reduced errors arising from fluctuations in the laser ...between single pulses and avoid the "overlap" of two or more ...
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Single and multi photon excited fluorescence from serotonin complexed with B cyclodextrin
... molecule from the surrounding environment as well as a means of improving aqueous solubility of hydrophobic ...organic molecules, 14 for example in permitting room temperature phosphorescence , 15 ...
15
Observing Biomolecular Dynamics from Nanoseconds to Hours with Single-Molecule Fluorescence Spectroscopy
... of single molecules (t D = ...adjacent molecules are ...arriving molecules at low and high inter- burst times, ...Recurrent molecules with static FRET should perfectly rebuild the ...
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Fluorescence-force spectroscopy at the single molecule level
... μm from the trap center and taking a confocal image around the tethered ...acceptor fluorescence intensities with 44ms time resolution as the applied force ramped ...cycles from 10-20 ...
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Hochrein, Marion (2005): From Single DNA Molecules to an Entire Virus: an Investigation with Quantitative Fluorescence Microscopy and X-Ray Reflectivity. Dissertation, LMU München: Fakultät für Physik
... 4 About Lipids and Membranes 4.1 Biomembranes Life as we know it is only possible because nature has invented membranes. Membranes enable the separation of living entities from the lifeless and possible hostile ...
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Monitoring and Manipulating Motions of Single Molecules/Nanoparticles.
... epi- fluorescence microscopy (shown in supplementary movie 1, 20 frames per second, or ...recognized from the movie: permanent adsorption, and free 3D Brownian ...
278
Analytical tools for single-molecule fluorescence imaging in cellulo
... labelled molecules in three spatial dimensions as a function of the fourth dimension of ...the fluorescence emission optical pathway prior to imaging on a camera ...away from the objective lens ...
16
Combined single-molecule force and fluorescence measurements for biology
... A single-molecule experiment is capable of detecting subpopulations or intermediates that may be impossible to observe by measuring the properties of an ...information from a single molecule, ...
5
Hards, Andrew (2004): The combined AFM manipulation and fluorescence imaging of single DNA molecules. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... Measuring the TIR field with the AFM cantilever In order to characterise the evanescent field, a method is presented to determine the field intensity by using an AFM cantilever as a local probe. The luminescence of a Si ...
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On-Demand Delivery of Single DNA Molecules Using Nanopipets
... a single nanopore, which can be used as single-molecule label-free sensors 7-9 , just like conventional solid-state ...for single cell interrogation 10,11 and as a macroscopic delivery vehicle for ...
24
Allele-specific detection of single mRNA molecules in situ
... concentration from 0%–25%, and we used probe concentrations in the range of ...inverted fluorescence microscope equipped with a 100× oil-immersion objective and a Photometrics Pixis 1024B CCD ...
11
Probing confined fields with single molecules and vice versa
... pronounced. This is similar to the case of the x-pattern (x, A). The intensity profiles of the vertical section match very well even though the molecule shows blinking during the image acquisition. Other conspicuous ...
9
Optical imaging and tracking of single molecules. in ultrahigh vacuum
... individual molecules and track their dynamics on well-defined surfaces is crucial for the understanding of these ...manipulating single molecules on surfaces with spatial resolutions down to the ...
22
Fluorescence studies of aromatic molecules in micelles
... fluorescence spectrum of pyrene and the absorption band of perylene, as shown in Figure 5.30, excitation energy transfer between these molecules can be expected. E[r] ...
188
Conductivity studies of single protein molecules
... distinction from the properties that depend on the environmental ...well-separated molecules on the gold surface. This testing can enable single-molecule measurements, despite the size of the ...
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Plasmon Enhanced Fluorescence (PEF) of High and Low Quantum Yield Molecules
... We saw throughout chapter 2 of this thesis, that several parameters in the nanoparticle are crucial in tuning the enhancement to maximize and diversify the applications of SEF. The question arises on how much the plasmon ...
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High-lying Triplet Excitons of Thermally. Activated Delayed Fluorescence Molecules
... mean absolute deviation for the eleven TADF molecules, of which S n is categorized as Rydberg and CT excitons in Fig. 3 in main text. MAD is the mean absolute deviation for the eighteen TADF molecules. type ...
46
Characterization of splicing mechanisms by single-molecule fluorescence
... used single-molecule prism-based TIRF microscopy to characterize the dynamics of human spliceosomal ...Our single-molecule fluorescence studies of human spliceosomal RNAs show that both previously ...
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