[PDF] Top 20 Allosteric control of simian virus 40 T-antigen binding to viral origin DNA.

... Nucleotide inhibition of origin DNA binding is observed in three different DNA-binding assays with immunopurified COS1 T antigen, biochemically purified lytic T antigen, and crude COSi c[r] ... See full document

... with T antigen and pol-prim to co- ordinate the assembly of the replication forks (reviewed in references 26 and ...initiates DNA synthesis on the leading- and lagging-strand templates by ... See full document

... SV40 origin of ...the origin-binding region including residues 121 to 135 is involved in A/T untwisting but not in IP ...two DNA sequences, as well as the reactions that take place at ... See full document

... Mutations in the phosphorylation sites of simian virus 40 SV40 T antigen alter its origin DNA-binding specificity for sites I and II and affect SV40 DNA replication activity.. Initiation[r] ... See full document

... does T antigen specifically unwind the site II sequence? Modeling of the presumptive double hexamer structure over 31-bp DNA using published structure information (3, 20, 25, 28, 29, 35, 36) shows ... See full document

... the T-Ag OBD and its interactions with ...the T-Ag OBD by nuclear magnetic resonance (NMR) meth- ods (30) and established that it is a monomer in ...the T-Ag OBD bound in a site-specific manner to a ... See full document

... The variants used to map the DNA-binding domain include: i fragments of large T produced by in vitro translation of unspliced SV40 cRNA 42; ii various truncated forms of large T present [r] ... See full document

... single-stranded DNA binding domain of large T antigen to amino acid residues 259 to ...this binding activity, a series of single-point substitutions were introduced in this domain, and ... See full document

... duplex DNA enters the central channel, and then at least one strand transits to the helicase surface via the side channels (23, 29, 44, ...the T-ag OBD ...in T-ag hexamers, and in other hex- americ ... See full document

... the DNA binding domain (residues 131 to 260) determined by nuclear magnetic resonance spectroscopy (29) reveals that three of the class 4 mutations and the class 5 mutation target a cluster of residues on ... See full document

... To investigate the specificity of pseudorevertant T antigens for different ori sequences, we constructed in vitro recombinants that contained mutant T antigen genes and various sequences[r] ... See full document

... of viral DNA replication, we examined the relationship between T-antigen oligomeric state and DNA helicase ...of T antigen were incubated with a model DNA ... See full document

... 66, 1992 ROLE OF T ANTIGEN AS A REPLICATIVE DNA HELICASE the viral origin of DNA replication, a bilobed T-antigen helicase complex was observed operating simultaneously at both forks of [r] ... See full document

... Small DNA tumor viruses such as simian virus 40 (SV40), polyomavirus, or papillomavirus have become important model systems for the study of molecular events involved in DNA ...large ... See full document

... to DNA showing that 70% of the protein-DNA contacts occur through these three nucleotides (4, ...reduced viral DNA replication by 5- to 10-fold, whereas mutations at positions 3, 4, and 5 ... See full document

... Specific binding to the unmethylated 1,229-base-pair fragment was not observed, even at higher T-antigen concentrations, nor was there binding to the smaller 222-base-pair plasmid fragme[r] ... See full document

... Similar treatment of the truncated 259.3 protein produced in HeLa cells resulted in only a slight if any increase in binding to both wt origin- and site Il-containing fragments.. Two int[r] ... See full document

... To obtain direct evidence that this region of the T antigen molecule is not only conformationally important but is, in fact, sufficient to act as the binding site with specificity for th[r] ... See full document

... core origin sequences, in addition to site II, that are required for T-ag assembly ...II-dependent T-ag assembly events, band shift experiments were conducted with a set of oligonucleotides termed ... See full document

... 73-82 0022-538X/82/070073-10$02.00/0 Discrete Regions of Simian Virus 40 Large T Antigen Are Required for Nonspecific and Viral Origin-Specific DNA Binding CAROL PRIVES,1* BETH BARNET,' [r] ... See full document